Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   HUW39_RS04165 Genome accession   NZ_CP056036
Coordinates   873522..875015 (+) Length   497 a.a.
NCBI ID   WP_000249328.1    Uniprot ID   -
Organism   Escherichia coli strain EC31     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 868522..880015
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUW39_RS04145 (HUW39_04145) pilD 869170..869979 (+) 810 WP_001547020.1 prepilin peptidase PppA Machinery gene
  HUW39_RS04150 (HUW39_04150) gspS2 870045..870455 (+) 411 WP_001300497.1 type II secretion system pilot lipoprotein GspS-beta -
  HUW39_RS04155 (HUW39_04155) gspC 870473..871432 (+) 960 WP_000135079.1 type II secretion system protein GspC -
  HUW39_RS04160 (HUW39_04160) gspD 871462..873522 (+) 2061 WP_001547019.1 type II secretion system secretin GspD -
  HUW39_RS04165 (HUW39_04165) pilB/pilB1 873522..875015 (+) 1494 WP_000249328.1 type II secretion system ATPase GspE Machinery gene
  HUW39_RS04170 (HUW39_04170) gspF 875015..876238 (+) 1224 WP_001173413.1 type II secretion system inner membrane protein GspF -
  HUW39_RS04175 (HUW39_04175) gspG 876255..876710 (+) 456 WP_001087289.1 type II secretion system major pseudopilin GspG -
  HUW39_RS04180 (HUW39_04180) gspH 876714..877277 (+) 564 WP_001115122.1 type II secretion system minor pseudopilin GspH -
  HUW39_RS04185 (HUW39_04185) gspI 877274..877645 (+) 372 WP_000820077.1 type II secretion system minor pseudopilin GspI -
  HUW39_RS04190 (HUW39_04190) gspJ 877642..878247 (+) 606 WP_001353918.1 type II secretion system minor pseudopilin GspJ -
  HUW39_RS04195 (HUW39_04195) gspK 878244..879212 (+) 969 WP_000633246.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55190.29 Da        Isoelectric Point: 6.0084

>NTDB_id=403150 HUW39_RS04165 WP_000249328.1 873522..875015(+) (pilB/pilB1) [Escherichia coli strain EC31]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSHELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIDGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQSAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=403150 HUW39_RS04165 WP_000249328.1 873522..875015(+) (pilB/pilB1) [Escherichia coli strain EC31]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTACAGGAGCTACAGCGGGGGCTGA
ATGCGCCCTTTACACTGCGGCAAATCGACGAGGCTGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAAGCTCGCCAGCTGATGGAAGATCTCGGTTCCGCCGAGGACTTTTTTACCCTCGCTGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCAATCAAAGAAGGCGCTT
CGGATATCCACATTGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGTTGCGC
CCCGGTCGTAAACTGGCCTCGCTGCTGGTCTCGCGTATCAAGGTGATGGCGCGACTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGCCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTCCGTGTATCTACCATGCCTTCTGCCTGGGGGG
AACGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCTCGCCTGACGCTGGAGCGTCTTGGTTTGAGTCACGAACTGACT
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATTTTTCTGGTGACGGGGCCGACCGGTTCCGGCAAAAGCACCAC
GCTGTACGCTGGATTGCAGGAGCTGAACAACCACTCGCGTAACATTCTCACGGTTGAAGACCCTATCGAATACATGATTG
ACGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACATTCGCCCGTGGCCTGCGCGCAATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCTTCACTGACCGGACACCT
GGTACTTTCCACGCTGCATACCAACACAGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGATTGCCGCCAGTCCGCGCCTGCC
ACCGACGAAGAAAAACGCCTGCTGGGAATTACCGATGCGCGAACCGTCACGCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAGCTGATCGTGGTAGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGTAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

48.111

79.879

0.384
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Acinetobacter baumannii D1279779

46.115

80.282

0.37
  pilB Vibrio cholerae strain A1552

45.96

79.678

0.366
  pilB Acinetobacter baylyi ADP1

46.154

78.471

0.362
  pilF Thermus thermophilus HB27

45

80.483

0.362

Multiple sequence alignment