Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FOB83_RS10100 Genome accession   NZ_CP046331
Coordinates   1993705..1995426 (-) Length   573 a.a.
NCBI ID   WP_011517604.1    Uniprot ID   Q1LIP6
Organism   Cupriavidus metallidurans strain FDAARGOS_675     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1988705..2000426
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOB83_RS10075 (FOB83_10075) - 1989626..1989817 (-) 192 WP_011517608.1 DNA gyrase inhibitor YacG -
  FOB83_RS10080 (FOB83_10080) zapD 1989826..1990584 (-) 759 WP_008650332.1 cell division protein ZapD -
  FOB83_RS10085 (FOB83_10085) coaE 1990807..1991451 (-) 645 WP_011517607.1 dephospho-CoA kinase -
  FOB83_RS10090 (FOB83_10090) pilD 1991522..1992391 (-) 870 WP_011517606.1 prepilin peptidase Machinery gene
  FOB83_RS10095 (FOB83_10095) - 1992410..1993675 (-) 1266 WP_011517605.1 type II secretion system F family protein -
  FOB83_RS10100 (FOB83_10100) pilB 1993705..1995426 (-) 1722 WP_011517604.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FOB83_RS10110 (FOB83_10110) ispB 1995899..1996828 (-) 930 WP_049799754.1 octaprenyl diphosphate synthase -
  FOB83_RS10115 (FOB83_10115) - 1996982..1997122 (+) 141 WP_155877846.1 hypothetical protein -
  FOB83_RS10120 (FOB83_10120) rplU 1997338..1997649 (+) 312 WP_008647259.1 50S ribosomal protein L21 -
  FOB83_RS10125 (FOB83_10125) rpmA 1997701..1997961 (+) 261 WP_008647258.1 50S ribosomal protein L27 -
  FOB83_RS10130 (FOB83_10130) obgE 1998087..1999184 (+) 1098 WP_008647251.1 GTPase ObgE -
  FOB83_RS10135 (FOB83_10135) proB 1999222..2000340 (+) 1119 WP_008647250.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62952.59 Da        Isoelectric Point: 7.5068

>NTDB_id=402760 FOB83_RS10100 WP_011517604.1 1993705..1995426(-) (pilB) [Cupriavidus metallidurans strain FDAARGOS_675]
MTLGLALAQSRRIAPALLAQLEQSAREKKTQLIDEIIGSGTMSAHDVAVFAADKYQLPLLDLSEYNLSRVPSALSGNREF
HAHRLLPLGRRENRLIVAMSDPANQAGVDVIRQKFSLPVETVVVEHDKLMKHVRSAGEALGTLKNALPTPTAQKMIEYDP
VAAASARKVPGANDIDDAPVVRFLQKLLTEAFHRGASDLHFEPFEFFYRIRFRVDGVLQEVARPPLDIRDKIATRIKVLS
RLDISEKRVPQDGRMKLLISMPKDKDGKETVDKAIDFRVSTLPTLFGEKIVMRILDSSSDKLDIDQLGYEPQQKALLQEV
IKRPYGMVLVTGPTGSGKTVSLYTFLNMLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDVIMV
GEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRLMNMGVAPFNIASSVLLITAQRLARRLCTCKREGDLAPQVLID
AGFQESDLDGTWKPYHPVGCDRCSGSGYKGRCGIYQVMPITETIQQIILSHGTALQIAEQARKEGVLSLREAGLLKVKQG
VTSLEEVLATTNT

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=402760 FOB83_RS10100 WP_011517604.1 1993705..1995426(-) (pilB) [Cupriavidus metallidurans strain FDAARGOS_675]
ATGACACTAGGCCTCGCGCTTGCCCAGAGCCGGCGGATCGCGCCGGCTCTGCTTGCCCAGTTGGAGCAATCGGCCCGGGA
AAAGAAGACGCAGCTGATCGACGAGATCATTGGCAGCGGCACGATGTCCGCCCATGACGTCGCCGTGTTCGCCGCCGACA
AGTACCAGCTCCCGCTGCTCGACCTCTCCGAATACAACCTCAGCCGCGTGCCGTCGGCACTCTCGGGCAACCGCGAGTTC
CATGCGCACCGGCTCCTGCCGCTGGGCCGTCGGGAAAACCGCCTGATCGTGGCGATGTCCGATCCGGCCAACCAGGCCGG
CGTCGACGTGATCCGCCAGAAGTTCAGCCTGCCAGTGGAAACAGTCGTCGTCGAACACGACAAGCTGATGAAGCATGTCC
GATCTGCCGGCGAGGCACTTGGCACGCTCAAGAACGCCCTGCCGACGCCCACGGCGCAGAAGATGATCGAGTATGACCCG
GTGGCCGCCGCGAGCGCGCGCAAGGTCCCCGGCGCCAACGACATCGACGACGCGCCGGTAGTGCGCTTCCTGCAGAAGCT
GCTGACGGAAGCCTTCCATCGCGGCGCGTCGGACCTTCACTTCGAACCATTCGAGTTCTTCTACCGCATTCGCTTCCGCG
TGGACGGCGTGCTGCAGGAAGTCGCGCGGCCACCGCTCGATATCCGCGACAAGATCGCCACGCGTATCAAGGTGCTATCG
CGCCTGGACATCTCGGAAAAGCGTGTGCCGCAGGACGGCCGGATGAAGCTACTGATCTCCATGCCGAAAGACAAGGACGG
CAAGGAGACCGTCGACAAGGCGATCGACTTCCGCGTCTCCACGCTGCCCACGTTGTTCGGCGAAAAGATCGTGATGCGGA
TTCTCGATTCGTCGAGCGACAAGCTCGACATCGACCAACTCGGCTATGAGCCGCAGCAGAAGGCACTGCTGCAGGAAGTG
ATCAAGCGGCCGTACGGGATGGTGCTGGTGACTGGCCCGACCGGCAGCGGCAAGACTGTGTCGCTCTATACGTTCCTGAA
CATGCTGAACCAGGGCGACATCAATATCTCGACCGCCGAGGATCCGGCCGAAATCCAGTTGCCCGGCATCAACCAGGTCA
ACGTGAACGACAAGGCCGGCCTGACCTTCGCCGCCGCGCTGAAATCGTTCCTGCGCCAGGATCCCGACGTGATCATGGTC
GGCGAAATTCGTGACCTCGAGACGGCCGACATCTCCATCAAGGCCGCGCAGACCGGTCACCTGGTGCTGTCCACGCTGCA
CACGAACGACGCGCCGACCACGCTGACACGCCTGATGAATATGGGCGTGGCACCGTTCAACATCGCCTCGTCGGTCCTGC
TGATCACCGCGCAGCGCCTGGCGCGCCGGCTTTGCACCTGCAAGCGCGAGGGCGACCTGGCACCGCAGGTGCTGATCGAT
GCAGGCTTCCAGGAGTCAGACCTCGACGGCACATGGAAGCCCTATCACCCGGTGGGCTGCGACCGATGCAGCGGTAGTGG
CTACAAGGGGCGATGCGGTATCTACCAGGTCATGCCGATTACCGAAACCATTCAACAGATCATCCTGTCGCACGGCACGG
CATTGCAGATTGCTGAACAGGCGCGCAAGGAAGGCGTGCTATCGTTGCGGGAAGCTGGCCTGCTCAAGGTCAAACAGGGC
GTCACGTCACTCGAAGAAGTGCTGGCGACCACGAACACGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(186-463)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q1LIP6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

51.923

99.825

0.518
  pilF Neisseria gonorrhoeae MS11

54.444

94.241

0.513
  pilB Acinetobacter baylyi ADP1

51.63

96.335

0.497
  pilB Legionella pneumophila strain ERS1305867

49.198

97.906

0.482
  pilB Vibrio cholerae strain A1552

45.614

99.476

0.454
  pilB Vibrio parahaemolyticus RIMD 2210633

46.642

93.543

0.436
  pilB Vibrio campbellii strain DS40M4

45.756

94.59

0.433
  pilF Thermus thermophilus HB27

38.185

100

0.389

Multiple sequence alignment