Detailed information NTG0402759

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: FOB83_RS10090
• Genome accession: NZ_CP046331
• Coordinates: 1991522..1992391 (-)
• Length: 289 a.a.
• NCBI ID: WP_011517606.1
• Uniprot ID: Q1LIP4
• Organism: Cupriavidus metallidurans strain FDAARGOS_675
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1986522..1997391

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FOB83_RS10065 (FOB83_10065)	-	1987011..1987454 (+)	444	WP_011517610.1	NUDIX domain-containing protein	-
FOB83_RS10070 (FOB83_10070)	-	1987510..1989540 (+)	2031	WP_011517609.1	OPT family oligopeptide transporter	-
FOB83_RS10075 (FOB83_10075)	-	1989626..1989817 (-)	192	WP_011517608.1	DNA gyrase inhibitor YacG	-
FOB83_RS10080 (FOB83_10080)	zapD	1989826..1990584 (-)	759	WP_008650332.1	cell division protein ZapD	-
FOB83_RS10085 (FOB83_10085)	coaE	1990807..1991451 (-)	645	WP_011517607.1	dephospho-CoA kinase	-
FOB83_RS10090 (FOB83_10090)	pilD	1991522..1992391 (-)	870	WP_011517606.1	prepilin peptidase	Machinery gene
FOB83_RS10095 (FOB83_10095)	-	1992410..1993675 (-)	1266	WP_011517605.1	type II secretion system F family protein	-
FOB83_RS10100 (FOB83_10100)	pilB	1993705..1995426 (-)	1722	WP_011517604.1	type IV-A pilus assembly ATPase PilB	Machinery gene
FOB83_RS10110 (FOB83_10110)	ispB	1995899..1996828 (-)	930	WP_049799754.1	octaprenyl diphosphate synthase	-
FOB83_RS10115 (FOB83_10115)	-	1996982..1997122 (+)	141	WP_155877846.1	hypothetical protein	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 31268.40 Da
• Isoelectric Point: 8.1375

>NTDB_id=402759 FOB83_RS10090 WP_011517606.1 1991522..1992391(-) (pilD) [Cupriavidus metallidurans strain FDAARGOS_675]
MQAVLSSLAALPPAFLIVAAGVVGLMVGSFLNVVIHRLPKMMEYDEANYIAEIRGEPLPHPERYNLMVPRSACPKCGHQI
APWENIPVISYLFLRGRCKACKTPIGIRYPLVELITAALSALAMWHFGPTAQALAAIVMTWSLVALFMIDADTQLLPDQI
TLPLLWLGLLLNLQGLFAPLADAVIGAAAGYLLLWSVYWLYKLVRGREGMGHGDFKLMAALGAWFGWQALPALILMSSVV
GAVLGGAMLVFRRKSSDTTFPFGPYIVGAGLIVLFFGADVLPLTLVAGH

Nucleotide

• Length: 870 bp

>NTDB_id=402759 FOB83_RS10090 WP_011517606.1 1991522..1992391(-) (pilD) [Cupriavidus metallidurans strain FDAARGOS_675]
ATGCAAGCTGTCCTGTCATCGCTGGCGGCGTTGCCGCCAGCTTTCCTTATTGTCGCGGCAGGGGTGGTTGGCCTGATGGT
CGGCAGTTTCCTCAACGTGGTGATCCATCGTCTGCCGAAGATGATGGAGTACGACGAGGCCAACTACATCGCGGAAATCC
GTGGCGAACCGTTGCCGCACCCCGAGCGCTATAACCTGATGGTGCCGCGATCCGCGTGCCCGAAGTGCGGCCACCAGATC
GCCCCCTGGGAGAACATCCCCGTCATCAGCTACCTGTTCCTGCGTGGCCGCTGCAAGGCCTGCAAGACGCCGATCGGCAT
CCGCTATCCGCTCGTCGAGCTGATCACGGCGGCGCTCAGCGCCCTGGCGATGTGGCACTTCGGTCCGACGGCGCAGGCGC
TGGCCGCGATCGTGATGACCTGGTCGCTGGTCGCGTTGTTCATGATCGATGCCGACACCCAGCTTCTGCCAGACCAGATC
ACGCTGCCGCTGCTATGGCTGGGCCTGCTGCTGAACCTGCAGGGCTTGTTCGCGCCGCTGGCGGATGCCGTCATCGGCGC
TGCGGCGGGCTACCTGCTGCTGTGGAGCGTCTACTGGCTCTACAAGCTCGTGCGGGGCCGCGAGGGCATGGGCCACGGCG
ATTTCAAGTTGATGGCGGCGCTTGGCGCATGGTTCGGCTGGCAGGCACTGCCCGCGCTGATCCTGATGTCGTCGGTGGTC
GGTGCCGTGCTTGGCGGCGCGATGCTGGTCTTCCGCCGCAAGAGTAGTGACACGACCTTCCCGTTTGGCCCGTACATCGT
CGGTGCCGGGCTGATCGTGCTGTTCTTTGGCGCAGACGTATTACCGCTGACGCTGGTGGCCGGACACTAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (138-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (22-127)

3D structure

• AlphaFold DB: Q1LIP4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.015	94.464	0.491
pilD	Vibrio campbellii strain DS40M4	52.206	94.118	0.491
pilD	Neisseria gonorrhoeae MS11	49.632	94.118	0.467
pilD	Acinetobacter baumannii D1279779	45.956	94.118	0.433
pilD	Acinetobacter nosocomialis M2	44.853	94.118	0.422