Detailed information NTG0401518

Bioinformatically predicted

Overview

• Name: dprA
• Type: Machinery gene
• Locus tag: GKC13_RS05220
• Genome accession: NZ_CP046134
• Coordinates: 1000489..1001328 (-)
• Length: 279 a.a.
• NCBI ID: WP_011227155.1
• Uniprot ID: A0A3G6JMX3
• Organism: Streptococcus thermophilus strain MAG_rmk202_sterm
• Function: ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 995489..1006328

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GKC13_RS05205 (GKC13_05235)	-	996540..997199 (-)	660	WP_011227158.1	response regulator transcription factor	-
GKC13_RS05210 (GKC13_05240)	-	997235..998059 (-)	825	WP_014621524.1	TraX family protein	-
GKC13_RS05215 (GKC13_05245)	topA	998177..1000321 (-)	2145	WP_179971534.1	type I DNA topoisomerase	-
GKC13_RS05220 (GKC13_05250)	dprA	1000489..1001328 (-)	840	WP_011227155.1	DNA-processing protein DprA	Machinery gene
GKC13_RS05225 (GKC13_05255)	-	1001396..1002316 (-)	921	WP_179971317.1	ROK family protein	-
GKC13_RS05230 (GKC13_05260)	-	1002327..1003094 (-)	768	WP_014608254.1	ribonuclease HII	-
GKC13_RS05235 (GKC13_05265)	ylqF	1003081..1003935 (-)	855	WP_014608253.1	ribosome biogenesis GTPase YlqF	-
GKC13_RS05240 (GKC13_05270)	-	1004063..1004752 (-)	690	WP_014608252.1	helix-turn-helix transcriptional regulator	-
GKC13_RS05245 (GKC13_05275)	-	1004847..1005362 (-)	516	WP_014608251.1	transcription repressor NadR	-
GKC13_RS05250 (GKC13_05280)	-	1005386..1005913 (-)	528	WP_011681081.1	ECF transporter S component	-

Sequence

Protein

• Length: 279 a.a.
• Molecular weight: 31233.11 Da
• Isoelectric Point: 8.4489

>NTDB_id=401518 GKC13_RS05220 WP_011227155.1 1000489..1001328(-) (dprA) [Streptococcus thermophilus strain MAG_rmk202_sterm]
MNNFELFKLKAAGLTNLNILNILDYQKNQDKKLSLRDMAVVSKSKNAVLFMEKYKNLDVNQLKEDFQRFPTLSIFDKEYP
IELKHSYNPPVLLFYQGNIDLLSKPKLAVVGARKCSETGKQSVRKIVHELGNAFTIVSGLARGIDTCAHMEALKNKGNTI
AVIGTGLDVYYPKENKALQDYMAKNHLVLTEYGPGEQPLKYHYPERNRIISGLCQGVMVVEAKLRSGSLITCERAMEEGR
DVFAIPGNILDGKSDGCHHLIQEGAKCITTGFDIISEFS

Nucleotide

• Length: 840 bp

>NTDB_id=401518 GKC13_RS05220 WP_011227155.1 1000489..1001328(-) (dprA) [Streptococcus thermophilus strain MAG_rmk202_sterm]
ATGAATAACTTTGAACTTTTTAAACTAAAAGCTGCTGGATTGACGAATTTAAACATCCTTAACATTTTAGACTATCAAAA
AAATCAAGATAAAAAATTGAGCTTACGTGATATGGCAGTGGTATCTAAGTCTAAAAATGCCGTTCTCTTTATGGAAAAAT
ACAAGAATTTAGATGTTAATCAGCTTAAGGAAGATTTTCAACGTTTTCCGACCTTATCAATTTTTGATAAGGAGTATCCC
ATTGAGCTGAAACATTCCTATAATCCTCCTGTCCTCCTCTTTTATCAAGGGAATATTGATTTGCTAAGTAAACCCAAGTT
AGCAGTAGTTGGTGCCAGAAAGTGTTCTGAAACAGGCAAACAGTCTGTTCGAAAAATTGTTCATGAACTTGGGAATGCCT
TTACTATTGTAAGTGGCTTGGCGCGTGGAATTGATACTTGTGCTCATATGGAGGCCTTAAAAAATAAAGGTAACACCATT
GCAGTTATCGGGACTGGGCTAGACGTATATTATCCTAAAGAAAACAAAGCTTTACAGGACTATATGGCTAAAAATCATTT
AGTTCTTACAGAATATGGACCGGGTGAACAGCCACTTAAATACCATTATCCTGAACGAAATCGGATAATTTCTGGATTGT
GTCAAGGGGTTATGGTGGTTGAAGCCAAGCTTCGCTCGGGAAGTCTTATTACTTGTGAGCGTGCAATGGAAGAAGGAAGA
GATGTTTTCGCTATACCAGGAAATATTTTAGATGGAAAATCGGATGGATGCCATCACCTCATTCAAGAGGGCGCAAAGTG
CATTACTACAGGATTTGACATCATTTCAGAATTTTCATAA

Domains

Predicted by InterproScan.

• DNA recombination-mediator protein A (DNA_processg_A) (71-269)

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (1-62)

3D structure

• AlphaFold DB: A0A3G6JMX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA	Streptococcus mutans UA159	71.685	100	0.717
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	63.309	99.642	0.631
dprA/cilB/dalA	Streptococcus pneumoniae D39	63.309	99.642	0.631
dprA/cilB/dalA	Streptococcus pneumoniae R6	63.309	99.642	0.631
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	63.309	99.642	0.631
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	62.95	99.642	0.627
dprA/cilB/dalA	Streptococcus mitis SK321	62.95	99.642	0.627
dprA	Lactococcus lactis subsp. cremoris KW2	60.072	99.642	0.599
dprA	Latilactobacillus sakei subsp. sakei 23K	39.768	92.832	0.369
dprA	Bacillus subtilis subsp. subtilis str. 168	48.095	75.269	0.362