Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   HUK75_RS24800 Genome accession   NZ_CP054792
Coordinates   5274259..5274801 (-) Length   180 a.a.
NCBI ID   WP_235179930.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain SE5431     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5269259..5279801
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUK75_RS24755 (HUK75_24700) - 5269568..5269816 (+) 249 WP_062832576.1 hypothetical protein -
  HUK75_RS24760 (HUK75_24705) - 5269791..5270237 (+) 447 WP_062832577.1 hypothetical protein -
  HUK75_RS24765 (HUK75_24710) - 5270463..5271179 (+) 717 WP_062832578.1 hypothetical protein -
  HUK75_RS24770 (HUK75_24720) - 5271172..5271447 (+) 276 WP_050714322.1 hypothetical protein -
  HUK75_RS24775 (HUK75_24725) - 5271665..5272132 (+) 468 WP_062832579.1 hypothetical protein -
  HUK75_RS24780 (HUK75_24730) - 5272126..5272506 (+) 381 WP_062832580.1 hypothetical protein -
  HUK75_RS24785 (HUK75_24735) - 5272597..5273064 (-) 468 WP_062832581.1 hypothetical protein -
  HUK75_RS24795 (HUK75_24745) - 5273498..5274196 (-) 699 WP_016254081.1 hypothetical protein -
  HUK75_RS24800 (HUK75_24750) pilE 5274259..5274801 (-) 543 WP_235179930.1 pilin Machinery gene
  HUK75_RS24805 (HUK75_24755) pilB 5275030..5276733 (+) 1704 WP_003141354.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HUK75_RS24810 (HUK75_24760) pilC 5276737..5277954 (+) 1218 WP_016254080.1 type II secretion system F family protein Machinery gene
  HUK75_RS24815 (HUK75_24765) pilD 5277957..5278829 (+) 873 WP_034007824.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  HUK75_RS24820 (HUK75_24770) coaE 5278826..5279437 (+) 612 WP_033989050.1 dephospho-CoA kinase -
  HUK75_RS24825 (HUK75_24775) yacG 5279434..5279634 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 18170.52 Da        Isoelectric Point: 5.0804

>NTDB_id=401360 HUK75_RS24800 WP_235179930.1 5274259..5274801(-) (pilE) [Pseudomonas aeruginosa strain SE5431]
MKAQKGFTLIELMIVVAIIGILAAVALPAYQDYTIRARVTEGVGLAASAKTLIGDSSATAGELAASARVWNAQAGNAGAT
SKYVTSVRIAEATGEITVTFNATNVGNIPADSSTLVFTPYVQNAAGAPTQLGASYAAGVTGSIDWGCASESNAVSSGPDR
NMPALTAGTLPARFAPSECR

Nucleotide


Download         Length: 543 bp        

>NTDB_id=401360 HUK75_RS24800 WP_235179930.1 5274259..5274801(-) (pilE) [Pseudomonas aeruginosa strain SE5431]
ATGAAAGCTCAGAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATTCTTGCTGCCGTCGCTTT
GCCGGCATATCAGGATTACACCATTCGTGCTCGCGTGACAGAGGGGGTTGGTCTGGCTGCCAGCGCCAAGACGCTTATTG
GCGATAGCTCTGCCACTGCCGGTGAGCTAGCCGCTTCGGCAAGGGTCTGGAATGCTCAAGCCGGTAACGCCGGTGCTACC
AGTAAGTATGTGACCTCTGTGCGAATTGCAGAGGCGACTGGTGAAATCACTGTTACTTTCAATGCCACAAACGTGGGTAA
TATTCCGGCTGACTCTTCTACCCTGGTATTTACTCCCTATGTGCAGAATGCTGCCGGTGCCCCGACTCAATTGGGTGCCA
GTTATGCTGCCGGTGTGACTGGCTCTATTGACTGGGGTTGTGCATCGGAATCCAATGCGGTTTCCAGTGGTCCCGACCGC
AATATGCCTGCCCTGACTGCAGGTACCCTGCCGGCTCGTTTCGCTCCGAGCGAATGCCGCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(36-180)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

43.147

100

0.472
  pilA Ralstonia pseudosolanacearum GMI1000

43.011

100

0.444
  pilA2 Legionella pneumophila str. Paris

41.573

98.889

0.411
  pilA2 Legionella pneumophila strain ERS1305867

41.011

98.889

0.406
  comP Acinetobacter baylyi ADP1

38.122

100

0.383