Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   GFU50_RS06445 Genome accession   NZ_CP046123
Coordinates   1359706..1360176 (+) Length   156 a.a.
NCBI ID   WP_154694346.1    Uniprot ID   -
Organism   Enterococcus casseliflavus strain EC291     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1348219..1393115 1359706..1360176 within 0


Gene organization within MGE regions


Location: 1348219..1393115
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFU50_RS06340 (GFU50_06340) - 1348219..1348641 (-) 423 WP_154694331.1 HIT family protein -
  GFU50_RS06345 (GFU50_06345) - 1349109..1350242 (-) 1134 WP_194186001.1 tyrosine-type recombinase/integrase -
  GFU50_RS06350 (GFU50_06350) - 1350341..1350754 (-) 414 WP_034872770.1 ImmA/IrrE family metallo-endopeptidase -
  GFU50_RS06355 (GFU50_06355) - 1350765..1351097 (-) 333 WP_194186000.1 helix-turn-helix domain-containing protein -
  GFU50_RS06360 (GFU50_06360) - 1351336..1351542 (+) 207 WP_412059277.1 helix-turn-helix domain-containing protein -
  GFU50_RS06365 (GFU50_06365) - 1351555..1351875 (+) 321 WP_144770898.1 DUF771 domain-containing protein -
  GFU50_RS06370 (GFU50_06370) - 1351999..1352232 (-) 234 WP_154694335.1 hypothetical protein -
  GFU50_RS06375 (GFU50_06375) - 1352308..1353057 (+) 750 WP_154694336.1 phage antirepressor KilAC domain-containing protein -
  GFU50_RS06380 (GFU50_06380) - 1353137..1353685 (-) 549 WP_121262166.1 hypothetical protein -
  GFU50_RS06385 (GFU50_06385) - 1353819..1354295 (-) 477 WP_154694337.1 hypothetical protein -
  GFU50_RS06390 (GFU50_06390) - 1354342..1354623 (+) 282 WP_154694338.1 hypothetical protein -
  GFU50_RS19030 - 1354799..1354921 (+) 123 WP_268259971.1 hypothetical protein -
  GFU50_RS06395 (GFU50_06395) - 1354922..1355182 (+) 261 WP_154694339.1 hypothetical protein -
  GFU50_RS06400 (GFU50_06400) - 1355269..1355604 (+) 336 WP_154694340.1 hypothetical protein -
  GFU50_RS06405 (GFU50_06405) - 1355601..1356245 (+) 645 WP_154694341.1 ERF family protein -
  GFU50_RS06410 (GFU50_06410) - 1356251..1356928 (+) 678 WP_154694342.1 putative HNHc nuclease -
  GFU50_RS06415 (GFU50_06415) - 1356948..1357721 (+) 774 WP_195907765.1 DnaD domain-containing protein -
  GFU50_RS06420 (GFU50_06420) - 1357726..1358562 (+) 837 WP_142962877.1 ATP-binding protein -
  GFU50_RS06425 (GFU50_06425) - 1358565..1358765 (+) 201 WP_154694344.1 hypothetical protein -
  GFU50_RS06430 (GFU50_06430) - 1358755..1359168 (+) 414 WP_142962879.1 hypothetical protein -
  GFU50_RS06435 (GFU50_06435) - 1359132..1359533 (+) 402 WP_230209443.1 RusA family crossover junction endodeoxyribonuclease -
  GFU50_RS06440 (GFU50_06440) - 1359530..1359709 (+) 180 WP_154694345.1 hypothetical protein -
  GFU50_RS06445 (GFU50_06445) ssbA 1359706..1360176 (+) 471 WP_154694346.1 single-stranded DNA-binding protein Machinery gene
  GFU50_RS18590 - 1360350..1360511 (+) 162 WP_195907756.1 hypothetical protein -
  GFU50_RS06450 (GFU50_06450) - 1360489..1361088 (+) 600 WP_154694347.1 DUF1642 domain-containing protein -
  GFU50_RS06455 (GFU50_06455) - 1361092..1361325 (+) 234 WP_154694348.1 hypothetical protein -
  GFU50_RS06460 (GFU50_06460) - 1361325..1361636 (+) 312 WP_230209444.1 hypothetical protein -
  GFU50_RS06465 (GFU50_06465) - 1361669..1362253 (+) 585 WP_230209445.1 hypothetical protein -
  GFU50_RS06470 (GFU50_06470) - 1362291..1362686 (+) 396 WP_154694350.1 hypothetical protein -
  GFU50_RS06475 (GFU50_06475) - 1362686..1363054 (+) 369 WP_230209446.1 hypothetical protein -
  GFU50_RS06480 (GFU50_06480) - 1363339..1363596 (+) 258 WP_154694351.1 hypothetical protein -
  GFU50_RS06485 (GFU50_06485) - 1363586..1363777 (+) 192 WP_154694352.1 hypothetical protein -
  GFU50_RS06490 (GFU50_06490) - 1363780..1363974 (+) 195 WP_154694353.1 hypothetical protein -
  GFU50_RS06495 (GFU50_06495) - 1363971..1364264 (+) 294 WP_230209447.1 hypothetical protein -
  GFU50_RS06500 (GFU50_06500) - 1364265..1364678 (+) 414 WP_113849809.1 hypothetical protein -
  GFU50_RS06505 (GFU50_06505) - 1365524..1366720 (+) 1197 WP_154694354.1 helix-turn-helix domain-containing protein -
  GFU50_RS06510 (GFU50_06510) - 1367208..1367687 (+) 480 WP_154694355.1 terminase small subunit -
  GFU50_RS06515 (GFU50_06515) - 1367680..1369002 (+) 1323 WP_154694356.1 PBSX family phage terminase large subunit -
  GFU50_RS06520 (GFU50_06520) - 1369016..1370434 (+) 1419 WP_154694357.1 phage portal protein -
  GFU50_RS06525 (GFU50_06525) - 1370424..1370729 (+) 306 WP_154694358.1 hypothetical protein -
  GFU50_RS06530 (GFU50_06530) - 1370742..1371890 (+) 1149 WP_154694359.1 minor capsid protein -
  GFU50_RS06535 (GFU50_06535) - 1371992..1372642 (+) 651 WP_228074064.1 DUF4355 domain-containing protein -
  GFU50_RS06540 (GFU50_06540) - 1372658..1373692 (+) 1035 WP_154694361.1 major capsid protein -
  GFU50_RS06545 (GFU50_06545) - 1373704..1374030 (+) 327 WP_154694362.1 phage head-tail connector protein -
  GFU50_RS06550 (GFU50_06550) - 1374011..1374361 (+) 351 WP_154694363.1 hypothetical protein -
  GFU50_RS06555 (GFU50_06555) - 1374351..1374893 (+) 543 WP_154694364.1 HK97 gp10 family phage protein -
  GFU50_RS06560 (GFU50_06560) - 1374893..1375255 (+) 363 WP_154694365.1 hypothetical protein -
  GFU50_RS06565 (GFU50_06565) - 1375268..1375690 (+) 423 WP_086317165.1 phage tail protein -
  GFU50_RS06570 (GFU50_06570) - 1375786..1376193 (+) 408 WP_154694366.1 DUF6096 family protein -
  GFU50_RS06575 (GFU50_06575) - 1376217..1376591 (+) 375 WP_154694367.1 hypothetical protein -
  GFU50_RS06580 (GFU50_06580) - 1376602..1381509 (+) 4908 WP_154694368.1 phage tail protein -
  GFU50_RS06585 (GFU50_06585) - 1381523..1381879 (+) 357 WP_010749498.1 DUF6711 family protein -
  GFU50_RS06590 (GFU50_06590) - 1381891..1385937 (+) 4047 WP_154694369.1 hypothetical protein -
  GFU50_RS06595 (GFU50_06595) - 1385948..1386370 (+) 423 WP_195907757.1 DUF1617 family protein -
  GFU50_RS06600 (GFU50_06600) - 1386413..1386661 (+) 249 WP_154694370.1 hypothetical protein -
  GFU50_RS06605 (GFU50_06605) - 1386696..1386989 (+) 294 WP_010749285.1 hypothetical protein -
  GFU50_RS06610 (GFU50_06610) - 1386991..1387236 (+) 246 WP_010749286.1 phage holin -
  GFU50_RS06615 (GFU50_06615) - 1387275..1388054 (+) 780 WP_154694371.1 N-acetylmuramoyl-L-alanine amidase family protein -
  GFU50_RS06620 (GFU50_06620) - 1388773..1389210 (+) 438 WP_230209448.1 Panacea domain-containing protein -
  GFU50_RS06625 (GFU50_06625) - 1389214..1389651 (+) 438 WP_154694372.1 hypothetical protein -
  GFU50_RS06630 (GFU50_06630) - 1389714..1390352 (-) 639 WP_154694373.1 hypothetical protein -
  GFU50_RS06635 (GFU50_06635) - 1390484..1390777 (-) 294 WP_154694374.1 hypothetical protein -
  GFU50_RS06640 (GFU50_06640) - 1390668..1390967 (-) 300 WP_195907758.1 hypothetical protein -
  GFU50_RS06645 (GFU50_06645) - 1390939..1391229 (-) 291 WP_154694376.1 DUF5960 family protein -
  GFU50_RS06650 (GFU50_06650) - 1391382..1391999 (-) 618 WP_154694377.1 DUF4352 domain-containing protein -
  GFU50_RS06660 (GFU50_06660) - 1392417..1393115 (-) 699 WP_005225745.1 O-methyltransferase -

Sequence


Protein


Download         Length: 156 a.a.        Molecular weight: 17188.85 Da        Isoelectric Point: 5.1932

>NTDB_id=401262 GFU50_RS06445 WP_154694346.1 1359706..1360176(+) (ssbA) [Enterococcus casseliflavus strain EC291]
MINNVVLVGRLTKDPDLKYTGSGTAVATFTLAVNRNFTNQSGEREADFINCVIWRKPAETLANYAKKGVLIGVTGRIQTR
SYDNQQGQKVYVTEVIADNFQLLESKKADSSQNTQGSGVSNSQTNNYARNQQNRNNDESDPFGNSSIDISDDSLPF

Nucleotide


Download         Length: 471 bp        

>NTDB_id=401262 GFU50_RS06445 WP_154694346.1 1359706..1360176(+) (ssbA) [Enterococcus casseliflavus strain EC291]
ATGATAAACAACGTTGTACTTGTCGGAAGACTGACGAAAGATCCAGATTTGAAATATACAGGTAGCGGAACCGCAGTTGC
CACCTTCACATTAGCTGTGAATCGCAACTTCACGAATCAGAGCGGAGAGCGAGAAGCGGACTTCATCAATTGTGTTATTT
GGAGAAAGCCAGCTGAAACATTAGCGAACTATGCGAAAAAAGGCGTGTTGATCGGAGTAACGGGGCGGATTCAAACTCGT
TCTTACGATAACCAACAAGGACAAAAAGTTTATGTCACCGAAGTGATTGCGGACAACTTCCAGTTGTTAGAAAGCAAGAA
AGCCGATTCTAGCCAAAATACACAAGGTAGCGGCGTTTCAAATAGTCAAACGAATAATTACGCTCGCAACCAACAAAACC
GCAACAACGATGAATCAGACCCATTTGGTAACTCGTCGATTGATATCAGTGATGATAGCCTTCCGTTTTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

58.046

100

0.647

  ssb Latilactobacillus sakei subsp. sakei 23K

58.14

100

0.641

  ssbB Bacillus subtilis subsp. subtilis str. 168

59.292

72.436

0.429

  ssbA Streptococcus mutans UA159

37.179

100

0.372

  ssbB/cilA Streptococcus pneumoniae TIGR4

53.774

67.949

0.365

  ssbB Streptococcus sobrinus strain NIDR 6715-7

48.718

75

0.365


Multiple sequence alignment