Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HUK70_RS10415 Genome accession   NZ_CP054789
Coordinates   2143429..2144937 (+) Length   502 a.a.
NCBI ID   WP_003091377.1    Uniprot ID   A0A0H2ZC25
Organism   Pseudomonas aeruginosa strain SE5381     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2138429..2149937
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUK70_RS10395 (HUK70_10340) - 2138459..2139670 (+) 1212 WP_003113933.1 O-succinylhomoserine sulfhydrylase -
  HUK70_RS10400 (HUK70_10345) - 2139667..2140434 (+) 768 WP_003103539.1 SDR family oxidoreductase -
  HUK70_RS10405 (HUK70_10350) xcpQ 2140521..2142497 (-) 1977 WP_003122269.1 GspD family T2SS secretin variant XcpQ -
  HUK70_RS10410 (HUK70_10355) xcpP 2142502..2143209 (-) 708 WP_003119750.1 type II secretion system protein N -
  HUK70_RS10415 (HUK70_10360) pilB 2143429..2144937 (+) 1509 WP_003091377.1 GspE family T2SS ATPase variant XcpR Machinery gene
  HUK70_RS10420 (HUK70_10365) xcpS 2144937..2146154 (+) 1218 WP_010793224.1 GspF family T2SS innner membrane protein variant XcpS -
  HUK70_RS10425 (HUK70_10370) xcpT 2146159..2146605 (+) 447 WP_003119751.1 GspG family T2SS major pseudopilin variant XcpT -
  HUK70_RS10430 (HUK70_10375) xcpU 2146612..2147130 (+) 519 WP_003103530.1 GspH family T2SS minor pseudopilin variant XcpU -
  HUK70_RS10435 (HUK70_10380) xcpV 2147127..2147516 (+) 390 WP_003103528.1 GspI family T2SS minor pseudopilin variant XcpV -
  HUK70_RS10440 (HUK70_10385) xcpW 2147513..2148217 (+) 705 WP_003091372.1 GspJ family T2SS minor pseudopilin variant XcpW -
  HUK70_RS10445 (HUK70_10390) xcpX 2148214..2149215 (+) 1002 WP_003103524.1 GspK family T2SS minor pseudopilin variant XcpX -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 55524.73 Da        Isoelectric Point: 6.2074

>NTDB_id=401019 HUK70_RS10415 WP_003091377.1 2143429..2144937(+) (pilB) [Pseudomonas aeruginosa strain SE5381]
MMTAPLPDIAAPAAPPRRLPFSFAKRQGLLFLCLEEQYWLACRPQVELAAIAEAQRFAGRRLPLKALGEDAFNQALAASY
QHDSSAAMQLAEDLGGSLDLAALADQVPETEDLMEQEDDAPIIRLINAILGEAIRENASDIHLETFEKRLVVRFRVDGVL
REVLEPKRELAALLVSRIKVMARLDIAEKRIPQDGRISLRVGGREVDIRVSTLPSANGERVVLRLLDKQAGRLNLQHLGM
SERDRKLMDETVRKPHGILLVTGPTGSGKTTTLYASLTTLNDRTRNILTVEDPIEYHLEGIGQTQVNAKVDMTFARGLRA
ILRQDPDVVMVGEIRDRETAEIAVQASLTGHLVLSTLHTNSAIGAITRLVDMGIEPFLLSSSMLGVLAQRLVRVLCPACK
EPYRADEAECALLGVDPAAPPTLHRARGCGECHQHGYRGRTGIYELVVFDDHMRSLIHNESSEQEMTRHARTSGPSIRDD
GRRKVLEGVTTVEEVLRVTREE

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=401019 HUK70_RS10415 WP_003091377.1 2143429..2144937(+) (pilB) [Pseudomonas aeruginosa strain SE5381]
ATGATGACAGCCCCATTACCCGACATCGCCGCGCCGGCCGCACCGCCACGGCGTCTGCCGTTCAGCTTCGCCAAGCGCCA
GGGCCTGCTGTTCCTGTGCCTGGAGGAGCAGTACTGGCTGGCCTGCCGGCCGCAGGTGGAGCTGGCGGCGATCGCCGAGG
CGCAGCGCTTCGCCGGCCGCCGCCTGCCGCTCAAGGCGCTCGGCGAGGACGCCTTCAACCAGGCCCTGGCGGCCTCCTAC
CAGCACGACTCCTCGGCGGCCATGCAGCTCGCCGAGGACCTCGGCGGCAGCCTGGACCTCGCCGCGCTGGCCGACCAGGT
CCCGGAAACCGAGGACCTGATGGAGCAGGAGGACGACGCGCCGATCATACGCCTGATCAACGCGATCCTCGGCGAGGCGA
TCCGCGAGAACGCCTCGGACATCCACCTGGAAACCTTCGAGAAACGCCTGGTGGTGCGCTTCCGCGTCGACGGCGTGCTG
CGCGAGGTGCTGGAGCCCAAGCGCGAGCTGGCGGCCCTGCTGGTATCGCGGATCAAGGTCATGGCGCGGCTGGACATCGC
CGAGAAGCGTATTCCCCAGGATGGGCGGATTTCCCTGCGGGTCGGCGGGCGCGAGGTGGACATCCGCGTCTCGACCCTGC
CCTCGGCCAACGGCGAACGGGTGGTGCTGCGTCTGCTCGACAAGCAGGCCGGGCGCCTGAACCTGCAACACCTGGGCATG
AGCGAGCGCGACCGCAAGCTGATGGACGAAACGGTGCGCAAGCCGCACGGCATCCTGCTGGTCACCGGGCCCACCGGCTC
GGGCAAGACCACCACCCTGTACGCCAGCCTGACCACGCTCAACGACCGCACGCGGAACATTCTCACCGTCGAGGATCCGA
TCGAATACCACCTGGAGGGCATCGGCCAGACCCAGGTCAACGCCAAGGTCGACATGACCTTCGCCCGCGGCCTGCGGGCG
ATCCTCCGGCAGGACCCGGACGTGGTGATGGTCGGCGAGATCCGCGACCGGGAGACCGCCGAGATCGCGGTGCAGGCTTC
GCTGACCGGCCACCTGGTGCTCTCGACCCTGCACACCAACAGCGCCATCGGTGCGATCACCCGCCTGGTGGACATGGGCA
TCGAGCCGTTCCTGCTGTCCTCCTCCATGCTTGGCGTGCTGGCCCAGCGCCTGGTGCGGGTGCTCTGCCCGGCGTGCAAG
GAGCCCTACCGCGCCGACGAGGCCGAGTGCGCGCTGCTCGGCGTCGATCCCGCCGCGCCGCCGACCCTGCATCGCGCCCG
CGGCTGCGGCGAGTGCCACCAGCATGGCTATCGCGGGCGTACCGGTATCTATGAACTGGTGGTGTTCGACGACCACATGC
GCTCGCTGATCCACAACGAATCCTCCGAGCAGGAAATGACCCGGCATGCCCGCACCAGCGGACCAAGCATCCGCGACGAC
GGTCGGCGCAAGGTGCTGGAAGGCGTCACCACGGTCGAGGAAGTCCTGCGCGTGACCCGGGAAGAATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZC25

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

43.532

97.012

0.422
  pilB/pilB1 Synechocystis sp. PCC 6803

42.245

97.61

0.412
  pilB Vibrio campbellii strain DS40M4

43.249

94.422

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

47.045

84.263

0.396
  pilF Thermus thermophilus HB27

43.207

89.442

0.386
  pilB Acinetobacter baumannii D1279779

47.88

79.88

0.382
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.446

76.892

0.373
  pilF Neisseria gonorrhoeae MS11

46.954

78.486

0.369
  pilB Acinetobacter baylyi ADP1

47.68

77.291

0.369
  pilB Legionella pneumophila strain ERS1305867

47.423

77.291

0.367