Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   GJV52_RS03365 Genome accession   NZ_CP046027
Coordinates   663843..665522 (-) Length   559 a.a.
NCBI ID   WP_100562517.1    Uniprot ID   -
Organism   Neisseria brasiliensis strain N.177.16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 658843..670522
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJV52_RS03335 (GJV52_03335) lspA 659290..659790 (+) 501 WP_095501897.1 signal peptidase II -
  GJV52_RS03340 (GJV52_03340) ispH 659815..660783 (+) 969 WP_095501898.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  GJV52_RS03345 (GJV52_03345) yacG 660865..661050 (-) 186 WP_095501899.1 DNA gyrase inhibitor YacG -
  GJV52_RS03350 (GJV52_03350) coaE 661053..661661 (-) 609 WP_100562515.1 dephospho-CoA kinase -
  GJV52_RS03355 (GJV52_03355) pilD 661662..662525 (-) 864 WP_095501901.1 A24 family peptidase Machinery gene
  GJV52_RS03360 (GJV52_03360) pilG 662527..663768 (-) 1242 WP_095501902.1 type II secretion system F family protein Machinery gene
  GJV52_RS03365 (GJV52_03365) pilF 663843..665522 (-) 1680 WP_100562517.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GJV52_RS03375 (GJV52_03375) - 666189..666755 (+) 567 WP_095501904.1 hypothetical protein -
  GJV52_RS03380 (GJV52_03380) groL 666881..668515 (-) 1635 WP_100562519.1 chaperonin GroEL -
  GJV52_RS03385 (GJV52_03385) groES 668588..668875 (-) 288 WP_095501906.1 co-chaperone GroES -
  GJV52_RS03390 (GJV52_03390) - 669205..670143 (+) 939 WP_157798095.1 alpha/beta hydrolase -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62250.61 Da        Isoelectric Point: 6.3941

>NTDB_id=400310 GJV52_RS03365 WP_100562517.1 663843..665522(-) (pilF) [Neisseria brasiliensis strain N.177.16]
MSVGLLRVLAQSQTITPTQAEHYQAAIKAHKDIMPMLFDDGVISPKALGELVARVFSYPLLDLHHYPRSHVLTDVLTEEQ
MVQNRCVPIFRRGRKVYLAVSDPTQIQNFQKIAFSSGVSVDLVVVPHDQLSSLLEWIGQRSTTILKEISQEQESSQTPQA
LYIDNEEAEDGPIPRFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISEK
RVPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDAATLNIDQLGFEPFQKEMLLEAIHRPYGMVLVTGPTGS
GKTVSLYTCLNILNTEDVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCPSCKKPIERPPVPALKKAGFTDEDLANDWQM
YRPVGCDSCRGKGFKGRVGVYEVMPITEEMQRVIMDNGTEVDIQNMAYQEGMVDLRRAGLLKVMQGLTSLEEVTAHTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=400310 GJV52_RS03365 WP_100562517.1 663843..665522(-) (pilF) [Neisseria brasiliensis strain N.177.16]
ATGAGTGTCGGATTACTGCGCGTCTTGGCGCAAAGTCAAACCATTACGCCTACTCAGGCAGAACATTATCAAGCAGCAAT
CAAAGCGCATAAAGACATTATGCCTATGCTGTTTGATGATGGTGTGATTTCTCCCAAAGCCCTTGGTGAGCTGGTGGCGC
GCGTGTTCAGCTATCCTTTGCTGGATTTGCACCATTATCCGCGTAGTCATGTCTTGACGGATGTGTTGACTGAAGAGCAG
ATGGTGCAAAACCGCTGTGTGCCGATTTTCCGTCGCGGCCGTAAGGTTTATTTGGCGGTTTCTGATCCGACTCAAATTCA
AAATTTTCAAAAAATCGCCTTTTCTTCAGGTGTTTCTGTCGATTTGGTGGTGGTGCCGCATGACCAGTTGAGCTCTTTAC
TGGAATGGATTGGTCAACGTTCGACTACCATTTTGAAAGAAATCAGCCAAGAGCAGGAATCTTCTCAAACACCGCAAGCG
TTGTATATCGACAATGAAGAAGCGGAAGATGGTCCGATTCCGCGTTTTATTCATAAAACATTGTCTGATGCCTTGAATGC
CGGTGCCTCAGATATCCATTTTGAATTTTACGAGCAAATGGCGCGTGTGCGTTTCCGTGTCGATGGCCAATTGCGTGAAG
TAGTGCAGCCGCCGGTTGCTGTGCGCGGTCAGTTGGCCTCACGTATTAAAGTGATGGCGCGCTTGGATATTTCTGAAAAA
CGCGTGCCGCAAGATGGTCGTATTCAGATTGCCTTCCATAAACATGGTCGCCCGATTGATTTCCGTGTGAGTACCTTGCC
GACTTTGTTCGGTGAAAAAGTGGTGATGCGTATTCTGAATTCGGATGCCGCCACATTGAATATCGACCAATTGGGCTTTG
AGCCATTCCAAAAAGAAATGCTGCTTGAAGCCATTCACCGTCCTTACGGTATGGTGCTGGTGACCGGCCCTACCGGCTCG
GGTAAAACTGTTTCGCTGTATACCTGCCTGAATATTTTGAACACGGAAGACGTGAATATTTCCACCGCGGAAGACCCTGC
CGAGATTAACTTGCCGGGAATTAATCAGGTTAACGTCAATGACAAACAAGGCCTGACCTTTGCGGCTGCGCTGAAATCTT
TCCTGCGTCAGGATCCGGACATCATCATGGTCGGCGAGATTCGTGACTTGGAAACTGCCGATATTGCGATTAAAGCCGCG
CAAACCGGTCATATGGTGTTTTCGACATTGCACACCAATAATGCACCGGCAACCTTGTCGCGTATGCTGAATATGGGCGT
GGCGCCTTTTAATATTGCCAGTTCGGTAAGTTTGATTATGGCGCAGCGTTTGTTGCGTCGCCTGTGCCCAAGTTGTAAAA
AACCGATTGAACGTCCGCCGGTTCCGGCATTGAAAAAAGCTGGGTTTACTGATGAAGATTTGGCCAATGATTGGCAAATG
TACCGTCCGGTTGGTTGTGACAGTTGTCGCGGCAAAGGTTTTAAAGGCCGTGTCGGTGTGTATGAAGTGATGCCGATTAC
CGAAGAAATGCAACGTGTGATTATGGATAACGGCACGGAAGTCGATATCCAAAACATGGCTTACCAAGAAGGCATGGTGG
ATTTGCGTCGTGCTGGTTTATTGAAAGTCATGCAGGGGTTGACTTCGTTGGAAGAAGTAACGGCACACACCAATGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(179-447)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

78.891

100

0.789
  pilB Acinetobacter baylyi ADP1

52.313

100

0.526
  pilB Acinetobacter baumannii D1279779

51.423

100

0.517
  pilB Legionella pneumophila strain ERS1305867

47.183

100

0.479
  pilB Vibrio parahaemolyticus RIMD 2210633

45.745

100

0.462
  pilB Vibrio cholerae strain A1552

45.374

100

0.456
  pilB Vibrio campbellii strain DS40M4

44.326

100

0.447
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.74

93.739

0.363

Multiple sequence alignment