Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   GJE05_RS07945 Genome accession   NZ_CP045960
Coordinates   1544318..1545994 (-) Length   558 a.a.
NCBI ID   WP_002228974.1    Uniprot ID   -
Organism   Neisseria meningitidis strain AUSMDU00005726     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1539318..1550994
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJE05_RS07925 (GJE05_07925) pilG 1541231..1542463 (+) 1233 WP_002216405.1 type 4 pilus assembly protein PilG Machinery gene
  GJE05_RS07930 (GJE05_07930) pilD 1542537..1543397 (+) 861 WP_002216403.1 prepilin peptidase Machinery gene
  GJE05_RS07935 (GJE05_07935) coaE 1543399..1544031 (+) 633 WP_002226983.1 dephospho-CoA kinase -
  GJE05_RS07940 (GJE05_07940) - 1544024..1544233 (+) 210 WP_002226982.1 DNA gyrase inhibitor YacG -
  GJE05_RS07945 (GJE05_07945) pilF 1544318..1545994 (-) 1677 WP_002228974.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GJE05_RS07960 (GJE05_07960) - 1546484..1546930 (-) 447 WP_002220271.1 DUF441 domain-containing protein -
  GJE05_RS07965 (GJE05_07965) ispB 1546935..1547909 (-) 975 WP_002216396.1 octaprenyl diphosphate synthase -
  GJE05_RS07975 (GJE05_07975) rplU 1548133..1548441 (+) 309 WP_002216394.1 50S ribosomal protein L21 -
  GJE05_RS07980 (GJE05_07980) rpmA 1548466..1548738 (+) 273 WP_002212328.1 50S ribosomal protein L27 -
  GJE05_RS07985 (GJE05_07985) ubiM 1549126..1550310 (+) 1185 WP_002216392.1 5-demethoxyubiquinol-8 5-hydroxylase UbiM -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 61961.09 Da        Isoelectric Point: 5.4180

>NTDB_id=399714 GJE05_RS07945 WP_002228974.1 1544318..1545994(-) (pilF) [Neisseria meningitidis strain AUSMDU00005726]
MSVGLLRILVQNQVVTVEQAEHYYKESQAGKEVLPILFSDGVISPKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQ
MVEFHCVPVFRRGDKVFFAVSDPTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPIAVRGQLASRIKVMSRLDISEKR
IPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSG
KTVSLYTCLNILNTESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQ
TGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKQEVERPSASALKEVGFTDEDLAKDWKLY
RAVGCDRCRGQGYKGRAGVYEVMPISEEMQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=399714 GJE05_RS07945 WP_002228974.1 1544318..1545994(-) (pilF) [Neisseria meningitidis strain AUSMDU00005726]
ATGAGCGTAGGTTTGCTGAGGATTCTGGTTCAAAACCAGGTGGTTACTGTTGAGCAGGCCGAGCATTACTACAAGGAGTC
GCAGGCGGGTAAGGAAGTGTTGCCGATACTGTTTTCAGACGGCGTCATTTCGCCCAAGTCGCTTGCGGCATTGATTGCAA
GGGTGTTCAGTTATTCGATTCTTGATTTGCGTCATTATCCGCGCCACAGGGTGCTGATGGGGGTGTTGACGGAGGAGCAG
ATGGTGGAGTTCCACTGTGTGCCGGTTTTCCGTCGGGGCGACAAAGTATTTTTTGCGGTTTCCGATCCGACACAGATGCC
GCAAATTCAGAAAACCGTTTCTGCCGCAGGGATTGAGGTTGAGTTGGTCATTGTCGAGGATGACCAGTTGGCGGGTTTGC
TCGATTGGGTGGGTTCGCGTTCGACATCGCTGCTTCAGGAGCTTGGGGAGGGGCAGGAGGAAGAGGAAAGCCACACCCTG
TATATCGACAACGAGGAGGCAGAAGACGGCCCTGTTCCGAGGTTTATCCATAAGACTTTGTCGGATGCCTTGCGCAGCGG
GGCATCGGACATCCATTTCGAGTTTTACGAACACAATGCCCGTATCCGTTTCCGTGTGGACGGGCAGCTCCGCGAGGTGG
TTCAGCCGCCCATTGCGGTAAGGGGGCAGCTTGCTTCCCGGATTAAGGTAATGTCGCGTTTGGACATTTCCGAAAAACGG
ATACCGCAGGACGGCAGGATGCAGCTGACCTTTCAAAAGGGCGGCAAGCCTGTCGATTTCCGTGTCAGCACATTGCCGAC
GCTGTTTGGCGAAAAGGTCGTGATGCGGATTTTGAATTCCGATGCCGCGTCTTTGAACATCGACCAGCTCGGTTTTGAGC
CGTTTCAGAAAAAATTGTTGTTGGAAGCGATTCACCGTCCCTACGGGATGGTGCTGGTAACCGGTCCGACGGGTTCGGGT
AAGACGGTGTCGCTCTATACCTGTTTGAATATTTTGAATACGGAGTCGGTAAATATTGCAACGGCGGAAGACCCTGCCGA
GATTAACCTGCCGGGCATCAATCAGGTTAACGTCAATGATAAGCAGGGCCTGACTTTTGCCGCTGCTTTGAAGTCTTTCC
TGCGTCAGGACCCGGACATCATTATGGTCGGTGAGATTCGTGATTTGGAAACTGCCGATATTGCGATTAAGGCGGCACAA
ACAGGGCATATGGTGTTTTCCACCCTGCACACCAATAATGCGCCGGCGACGTTGTCGCGTATGCTGAATATGGGTGTCGC
GCCGTTTAATATTGCCAGTTCGGTCAGCCTGATTATGGCGCAGCGTCTTTTACGCAGGCTGTGTTCGAGCTGCAAACAGG
AAGTGGAACGCCCGTCTGCCTCTGCTTTGAAGGAAGTCGGCTTCACCGATGAGGACCTTGCAAAAGATTGGAAACTTTAC
CGCGCCGTCGGTTGCGACCGTTGCCGGGGGCAGGGTTATAAGGGGCGTGCGGGCGTGTATGAGGTTATGCCCATCAGCGA
AGAAATGCAGCGTGTGATTATGAACAACGGTACGGAAGTGGATATTTTGGACGTTGCCTATAAGGAGGGTATGGTGGATT
TGCGCCGGGCCGGTATTTTGAAAGTTATGCAGGGCATTACTTCATTGGAAGAGGTAACGGCAAATACCAACGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(178-446)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

99.104

100

0.991
  pilB Acinetobacter baumannii D1279779

50.265

100

0.511
  pilB Acinetobacter baylyi ADP1

50.356

100

0.507
  pilB Legionella pneumophila strain ERS1305867

47.703

100

0.484
  pilB Vibrio parahaemolyticus RIMD 2210633

45.39

100

0.459
  pilB Vibrio cholerae strain A1552

45.374

100

0.457
  pilB Vibrio campbellii strain DS40M4

43.838

100

0.446
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.439

95.878

0.378
  pilF Thermus thermophilus HB27

37.132

97.491

0.362

Multiple sequence alignment