Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   GJE05_RS07930 Genome accession   NZ_CP045960
Coordinates   1542537..1543397 (+) Length   286 a.a.
NCBI ID   WP_002216403.1    Uniprot ID   A0A9K2PRG6
Organism   Neisseria meningitidis strain AUSMDU00005726     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1537537..1548397
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJE05_RS07915 (GJE05_07915) dapD 1538090..1538911 (-) 822 WP_002228980.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -
  GJE05_RS07920 (GJE05_07920) pgi 1539078..1540721 (-) 1644 WP_002228985.1 glucose-6-phosphate isomerase -
  GJE05_RS07925 (GJE05_07925) pilG 1541231..1542463 (+) 1233 WP_002216405.1 type 4 pilus assembly protein PilG Machinery gene
  GJE05_RS07930 (GJE05_07930) pilD 1542537..1543397 (+) 861 WP_002216403.1 prepilin peptidase Machinery gene
  GJE05_RS07935 (GJE05_07935) coaE 1543399..1544031 (+) 633 WP_002226983.1 dephospho-CoA kinase -
  GJE05_RS07940 (GJE05_07940) - 1544024..1544233 (+) 210 WP_002226982.1 DNA gyrase inhibitor YacG -
  GJE05_RS07945 (GJE05_07945) pilF 1544318..1545994 (-) 1677 WP_002228974.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GJE05_RS07960 (GJE05_07960) - 1546484..1546930 (-) 447 WP_002220271.1 DUF441 domain-containing protein -
  GJE05_RS07965 (GJE05_07965) ispB 1546935..1547909 (-) 975 WP_002216396.1 octaprenyl diphosphate synthase -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 31345.37 Da        Isoelectric Point: 8.2213

>NTDB_id=399713 GJE05_RS07930 WP_002216403.1 1542537..1543397(+) (pilD) [Neisseria meningitidis strain AUSMDU00005726]
MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVFAKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAW
QNIPIVSYLLLRGKCASCQTKISIRYPLIELLTGVLFGLVAWQYGWSWITLGGLVLTAFLISLTFIDADTQYLPDSMTLP
LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAALGAWLGISALPVLIFVSSLIGLV
AAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRAVNWWLTHPVL

Nucleotide


Download         Length: 861 bp        

>NTDB_id=399713 GJE05_RS07930 WP_002216403.1 1542537..1543397(+) (pilD) [Neisseria meningitidis strain AUSMDU00005726]
ATGTCTGATTTGTCTGTATTGTCGCCGTTTGCCGTGCCTTTGGCAGCGGTGTTCGGGCTGCTGGTCGGCAGTTTCTTAAA
CGTCGTCATTTACCGCGTGCCGGTCATGATGGAACGCGGCTGGACGGTATTTGCCAAAGAATATTTAAACCTGCCGCTGA
CCGAAGAGGAAAGCCGTACCTTCAACCTGATGAAACCGGATTCCTGCTGTCCCAAATGCCGCGTGCCGATACGCGCGTGG
CAGAACATCCCGATTGTCAGTTACCTGCTCCTGCGCGGCAAATGCGCCTCCTGTCAAACCAAAATCAGCATACGTTATCC
CTTAATCGAGCTGCTGACCGGCGTATTGTTCGGGCTGGTCGCCTGGCAATACGGCTGGTCTTGGATTACGCTGGGCGGAT
TGGTACTGACCGCGTTTCTGATTTCCCTGACCTTTATCGATGCGGACACCCAATACCTGCCCGACTCGATGACACTGCCC
TTGATTTGGCTGGGGCTGATATTTAATTTGGACGGCGGCTTCGTGCCTTTGCAGTCTGCCGTTTTAGGTGCGGTTGCCGG
CTATGGTTCGATATGGCTTTTATGCGCGGTATATAAACTGATTAAAGGCGAAATCGGTATGGGCAACGGAGATTTCAAAC
TGATTGCCGCATTGGGCGCGTGGCTCGGCATATCCGCATTGCCCGTGCTGATTTTTGTTTCCTCGCTGATCGGTTTGGTC
GCGGCAATCGTTATGCGCGTCGCCAAGGGGCAGCATTTTGCCTTCGGCCCCGCACTGACAGTTTCGGGCTGGATAATTTT
TACGGCAAACGATTCCGTATGGCGGGCGGTCAACTGGTGGCTGACCCATCCGGTGCTGTAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-124)

Type IV leader peptidase family (Peptidase_A24)

(134-242)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

95.789

99.65

0.955
  pilD Vibrio cholerae strain A1552

44.444

100

0.448
  pilD Acinetobacter baumannii D1279779

47.348

92.308

0.437
  pilD Acinetobacter nosocomialis M2

47.348

92.308

0.437
  pilD Vibrio campbellii strain DS40M4

44.928

96.503

0.434

Multiple sequence alignment