Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACAV_RS17810 Genome accession   NC_015138
Coordinates   4072978..4074714 (+) Length   578 a.a.
NCBI ID   WP_013595977.1    Uniprot ID   F0QCL1
Organism   Paracidovorax avenae ATCC 19860     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4067978..4079714
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAV_RS17780 (Acav_3590) proB 4068389..4069528 (-) 1140 WP_013595973.1 glutamate 5-kinase -
  ACAV_RS17785 (Acav_3591) cgtA 4069613..4070686 (-) 1074 WP_013595974.1 Obg family GTPase CgtA -
  ACAV_RS17790 (Acav_3592) rpmA 4070812..4071069 (-) 258 WP_013595975.1 50S ribosomal protein L27 -
  ACAV_RS17795 (Acav_3593) rplU 4071082..4071393 (-) 312 WP_011796717.1 50S ribosomal protein L21 -
  ACAV_RS17800 (Acav_3594) - 4071579..4072559 (+) 981 WP_013595976.1 polyprenyl synthetase family protein -
  ACAV_RS17810 (Acav_3595) pilB 4072978..4074714 (+) 1737 WP_013595977.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACAV_RS17815 (Acav_3596) pilC 4074746..4075963 (+) 1218 WP_013595978.1 type II secretion system F family protein Machinery gene
  ACAV_RS17820 (Acav_3597) - 4075963..4076850 (+) 888 WP_013595979.1 A24 family peptidase -
  ACAV_RS17825 (Acav_3598) coaE 4076847..4077473 (+) 627 WP_013595980.1 dephospho-CoA kinase -
  ACAV_RS17830 (Acav_3599) zapD 4077538..4078293 (+) 756 WP_041829314.1 cell division protein ZapD -
  ACAV_RS17835 (Acav_3600) - 4078320..4078574 (+) 255 WP_013595982.1 DNA gyrase inhibitor YacG -
  ACAV_RS17840 (Acav_3601) - 4078642..4079097 (-) 456 WP_013595983.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 63284.52 Da        Isoelectric Point: 5.3406

>NTDB_id=39874 ACAV_RS17810 WP_013595977.1 4072978..4074714(+) (pilB) [Paracidovorax avenae ATCC 19860]
MADADYALKDASSIALPGLGRALISAGQLSQKVAEDIYRKSQVNRSSFIAELTGSGSVSPINLAHTVSSVFGAPLLDIEA
IDPLRLPRDVLDPKICQAYRVIVLSKRNNRLIVATADPTDQEAAEKIKFTTQMGVDWIIAEYDKLVRLVDATTKSTTETM
ESMVSGGDFEFDDLPSQESTEAQDTAATEVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIASPP
IAIKEKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPEEKE
RLLHAIGRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFATALKSFLRQDP
DIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPACKAPADIP
HETLLEAGYKEEDIDGSWVTYRPVGCSACNNGYKGRVGIYQVMPISEEIQRIILRDGSALEIAEQAKAEGVRSLRESGLH
KARLGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=39874 ACAV_RS17810 WP_013595977.1 4072978..4074714(+) (pilB) [Paracidovorax avenae ATCC 19860]
ATGGCTGACGCCGATTACGCCCTCAAGGATGCTTCCAGTATCGCCCTGCCCGGCCTGGGGCGGGCATTGATCTCGGCCGG
GCAGCTGAGCCAGAAGGTGGCGGAGGACATCTACCGGAAGTCCCAGGTCAACCGCAGCAGCTTCATCGCGGAGCTGACCG
GTTCGGGTTCGGTCTCCCCGATCAACCTGGCGCATACCGTGTCGTCGGTGTTCGGCGCCCCCCTGCTCGACATCGAGGCC
ATCGATCCGCTGCGCCTGCCGCGCGACGTGCTGGACCCGAAGATCTGCCAGGCGTACCGGGTCATCGTGCTGAGCAAGCG
CAACAACCGGCTCATCGTCGCGACGGCAGACCCCACCGACCAGGAGGCTGCGGAGAAGATCAAGTTCACCACCCAGATGG
GCGTGGACTGGATCATCGCCGAGTACGACAAGCTGGTGCGGCTGGTGGATGCGACCACCAAGTCCACGACCGAGACCATG
GAAAGCATGGTGTCGGGGGGCGATTTCGAGTTCGATGACCTGCCGTCGCAGGAGAGTACCGAAGCGCAGGACACGGCCGC
GACGGAGGTCGAAGACGCCCCCATCGTCAAGTTCCTGCACAAGATGCTGCTGGACGCGTTCAACATGCGCGCGTCCGACC
TGCACTTCGAGCCGTACGAGCACCAGTACCGGGTCCGCTTCCGGATCGACGGCGAGCTGCGCGAGATCGCTTCGCCGCCC
ATCGCGATCAAGGAGAAGCTGGCCTCGCGCATCAAGGTGATCTCCCGCCTGGACATCTCGGAAAAGCGCGTGCCCCAGGA
CGGGCGCATGAAGCTCAAGGTGGGACCGGACCGGGTGATCGACTTCCGCGTGAGCACGCTGCCCACGCTGTTCGGAGAGA
AGATCGTGATCCGTATCCTCGACCCCAGCAGCGCCAAGCTGGGCATCGATGCGCTCGGCTACGAGCCCGAGGAGAAGGAG
CGCCTGCTCCATGCCATCGGCCGGCCCTACGGCATGATCCTGGTGACGGGACCTACGGGCTCGGGCAAGACGGTGTCGCT
CTACACCTGCCTGAACCTGCTGAACAAGCCGGGGGTGAACATCGCGACGGCGGAAGACCCGTCGGAAATCAACCTGCCCG
GTGTCAACCAGGTCAACGTGAACGAAAAGGCCGGGCTGACCTTCGCCACGGCGCTCAAGTCGTTCCTGCGGCAGGATCCC
GACATCATCATGGTGGGGGAAATCCGCGACCTGGAAACCGCGGACATCTCGATCAAGGCGGCCCAGACGGGCCACCTGGT
GCTCTCCACGCTGCACACCAACGACGCGCCCACCACGCTGACCCGCATGCGCAACATGGGCATCGCGCCCTTCAACATCG
CATCGAGCGTGATCCTGATCACGGCGCAGCGCCTGGCCCGCCGGCTGTGCCCTGCGTGCAAGGCGCCGGCGGACATCCCC
CACGAGACGCTGCTCGAGGCGGGCTACAAGGAAGAGGACATCGACGGCTCCTGGGTGACCTACCGCCCGGTGGGCTGTTC
CGCCTGCAACAACGGCTACAAGGGCCGCGTCGGCATCTACCAGGTCATGCCGATCTCGGAGGAGATCCAGCGCATCATCC
TGCGCGACGGCAGCGCGCTGGAAATCGCGGAGCAGGCAAAGGCCGAAGGCGTGCGGTCGCTGCGCGAATCCGGACTCCAC
AAGGCGCGGCTGGGCCTGACCTCGCTCGAAGAGGTGCTGGCCGTGACGAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(56-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F0QCL1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.833

98.443

0.54
  pilB Acinetobacter baumannii D1279779

53.054

99.135

0.526
  pilF Neisseria gonorrhoeae MS11

52.837

97.578

0.516
  pilB Legionella pneumophila strain ERS1305867

49.468

97.578

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

46.369

92.907

0.431
  pilB Vibrio campbellii strain DS40M4

44.385

97.059

0.431
  pilB Vibrio cholerae strain A1552

47.238

90.83

0.429
  pilF Thermus thermophilus HB27

39.145

100

0.396
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.199

99.308

0.389

Multiple sequence alignment