Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GH771_RS20975 Genome accession   NZ_CP045769
Coordinates   4477640..4478620 (+) Length   326 a.a.
NCBI ID   WP_088208561.1    Uniprot ID   -
Organism   Enterobacter cancerogenus strain MiY-F     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4472640..4483620
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GH771_RS20935 (GH771_20935) - 4472701..4473027 (+) 327 WP_153689416.1 YggL family protein -
  GH771_RS20940 (GH771_20940) - 4473085..4473801 (+) 717 WP_006178454.1 DUF2884 domain-containing protein -
  GH771_RS20945 (GH771_20945) - 4473906..4474244 (-) 339 WP_137272707.1 endonuclease domain-containing protein -
  GH771_RS20950 (GH771_20950) hemW 4474308..4475450 (-) 1143 WP_058608625.1 radical SAM family heme chaperone HemW -
  GH771_RS20955 (GH771_20955) - 4475443..4476036 (-) 594 WP_058608624.1 XTP/dITP diphosphatase -
  GH771_RS20960 (GH771_20960) yggU 4476040..4476336 (-) 297 WP_006178450.1 DUF167 family protein YggU -
  GH771_RS20965 (GH771_20965) - 4476333..4476899 (-) 567 WP_058608623.1 YggT family protein -
  GH771_RS20970 (GH771_20970) - 4476921..4477622 (-) 702 WP_153689417.1 YggS family pyridoxal phosphate-dependent enzyme -
  GH771_RS20975 (GH771_20975) pilT 4477640..4478620 (+) 981 WP_088208561.1 type IV pilus twitching motility protein PilT Machinery gene
  GH771_RS20980 (GH771_20980) ruvX 4478652..4479068 (-) 417 WP_006178446.1 Holliday junction resolvase RuvX -
  GH771_RS20985 (GH771_20985) - 4479068..4479628 (-) 561 WP_006178445.1 YqgE/AlgH family protein -
  GH771_RS20990 (GH771_20990) gshB 4479711..4480658 (-) 948 WP_042322311.1 glutathione synthase -
  GH771_RS20995 (GH771_20995) rsmE 4480680..4481411 (-) 732 WP_042321930.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  GH771_RS21000 (GH771_21000) endA 4481464..4482171 (-) 708 WP_006178442.1 deoxyribonuclease I -
  GH771_RS21005 (GH771_21005) - 4482266..4482763 (-) 498 WP_153689418.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35770.77 Da        Isoelectric Point: 6.0414

>NTDB_id=397010 GH771_RS20975 WP_088208561.1 4477640..4478620(+) (pilT) [Enterobacter cancerogenus strain MiY-F]
MDVEEIVALSVKHNVSDLHLCSDAPPRWRRLGRLEPAPFPPPDVNVLLREWLNDEQQGTWWANGQVDFAVTTGENQRLRG
SAFMHMQGASVALRLLPRACPQLSALGVPRAIPELLLNDSGLLLVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FIYQSERCLIQQREIGQHSPTFAEALRGALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRTQLAGSLRAVLAQKLRPDLQGGRVALYELLVNTPAAANLIREGKTWQLPGIVQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=397010 GH771_RS20975 WP_088208561.1 4477640..4478620(+) (pilT) [Enterobacter cancerogenus strain MiY-F]
ATGGATGTGGAAGAAATCGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATGCCCCCCCGCG
CTGGCGGCGATTAGGCCGCCTTGAGCCCGCCCCGTTTCCGCCTCCGGACGTGAACGTGCTGCTCAGGGAGTGGCTTAATG
ATGAACAGCAGGGAACGTGGTGGGCAAACGGGCAGGTGGATTTTGCCGTCACCACCGGCGAAAACCAGCGCCTGCGCGGC
AGCGCGTTTATGCATATGCAGGGTGCCTCTGTCGCGCTGCGACTGCTGCCGCGTGCCTGCCCGCAACTTTCTGCGCTGGG
CGTGCCGCGGGCGATCCCGGAGCTGTTACTGAATGACAGCGGGCTGCTGCTGGTGACCGGGGCAACCGGCAGCGGAAAAT
CCACCACTCTGGCGGCGATGGTCGATTTTCTCAACCACCACACCGACGGGCATATTCTGACGCTCGAAGATCCGGTGGAA
TTTATCTATCAGAGTGAACGCTGCCTGATCCAGCAGCGCGAGATAGGCCAGCACAGCCCGACGTTTGCCGAGGCGCTGCG
GGGCGCGTTGCGTGAAGATCCGGATGTGATTTTGTTGGGGGAGCTGCGCGATAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCCATTGAACGGCTGGTGGATACC
TTTCCGGCGCAGGAGAAAGACCCGGTGCGCACCCAGCTGGCGGGCAGCCTGCGGGCGGTTCTGGCGCAAAAGCTGCGCCC
GGATTTGCAGGGCGGGCGCGTCGCGCTTTATGAGCTGCTGGTGAATACCCCGGCGGCGGCGAACCTGATCCGTGAAGGCA
AAACCTGGCAGCTTCCCGGGATCGTTCAAACGGGTCAGCAGGCGGGCATGCAGAACTTTGAACAGAGCCTGGCTGAGCGC
CGGGCGCAGGGCAGGCTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Legionella pneumophila strain Lp02

47.516

98.773

0.469
  pilT Legionella pneumophila strain ERS1305867

47.516

98.773

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Neisseria meningitidis 8013

46.341

100

0.466
  pilT Acinetobacter nosocomialis M2

47.188

98.16

0.463
  pilT Neisseria gonorrhoeae MS11

46.037

100

0.463
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.236

98.773

0.417
  pilU Pseudomonas stutzeri DSM 10701

39.091

100

0.396
  pilU Vibrio cholerae strain A1552

37.888

98.773

0.374

Multiple sequence alignment