Detailed information NTG0395910

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: GFH30_RS06770
• Genome accession: NZ_CP045650
• Coordinates: 1440486..1441679 (-)
• Length: 397 a.a.
• NCBI ID: WP_153371504.1
• Uniprot ID: A0A5Q0P3W7
• Organism: Acinetobacter wanghuae strain dk386
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1435486..1446679

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GFH30_RS06750 (GFH30_06745)	-	1436946..1437395 (+)	450	WP_153371500.1	MaoC family dehydratase	-
GFH30_RS06755 (GFH30_06750)	-	1437546..1438574 (+)	1029	WP_153371501.1	AraC family transcriptional regulator	-
GFH30_RS06760 (GFH30_06755)	gcvH	1438657..1439031 (+)	375	WP_153371502.1	glycine cleavage system protein GcvH	-
GFH30_RS06765 (GFH30_06760)	-	1439259..1440437 (+)	1179	WP_153371503.1	MFS transporter	-
GFH30_RS06770 (GFH30_06765)	pilU	1440486..1441679 (-)	1194	WP_153371504.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
GFH30_RS06775 (GFH30_06770)	-	1441928..1442977 (-)	1050	WP_153371505.1	3-deoxy-7-phosphoheptulonate synthase	-
GFH30_RS06780 (GFH30_06775)	hppD	1444042..1445103 (+)	1062	WP_153371506.1	4-hydroxyphenylpyruvate dioxygenase	-

Sequence

Protein

• Length: 397 a.a.
• Molecular weight: 44996.32 Da
• Isoelectric Point: 6.2781

>NTDB_id=395910 GFH30_RS06770 WP_153371504.1 1440486..1441679(-) (pilU) [Acinetobacter wanghuae strain dk386]
MFTAELLEEAQRFMHHMLTKVVEYGGSDLFITADFPPSIKHQGLMKPFGQQTLTAEKTKLFAYGLMNERQRKEFETELEC
NFAITVPGVSRFRVNVFQQQLNVGMVIRTITAEIPNFQKLKLPESLKSVIMEKRGLVLVVGGTGSGKSTSLAAMIDYRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
SNNANQTLDRIINFFPEERRNQLLMDLSSNMKAIISQRLIRTEDGQGRRAAIEIMLNTPLISDLILKGEFHELKGIMSKS
RELGMQTFDQALFDLYNEGAISYEEALRNADSTNELRLQIKLKGQRRHASQDAAATAFTMYEEPKKEENEDENQESA

Nucleotide

• Length: 1194 bp

>NTDB_id=395910 GFH30_RS06770 WP_153371504.1 1440486..1441679(-) (pilU) [Acinetobacter wanghuae strain dk386]
ATGTTTACAGCAGAACTATTAGAAGAAGCACAACGCTTTATGCATCATATGCTAACCAAAGTGGTTGAATACGGCGGTTC
GGATCTGTTTATCACGGCTGATTTTCCACCCAGTATTAAGCATCAAGGTTTGATGAAACCTTTCGGGCAACAGACATTGA
CCGCGGAGAAAACCAAGCTATTTGCCTATGGTTTAATGAATGAACGGCAACGTAAAGAATTCGAAACAGAGCTTGAGTGT
AATTTTGCGATTACCGTTCCCGGTGTATCGCGTTTTCGCGTGAATGTGTTTCAACAACAGTTGAATGTAGGCATGGTGAT
TCGAACCATTACTGCAGAAATTCCAAATTTTCAAAAACTTAAACTTCCAGAGTCACTTAAATCCGTAATTATGGAAAAAC
GTGGGTTAGTACTTGTTGTCGGTGGCACGGGTTCAGGTAAATCAACCTCACTGGCTGCCATGATCGACTACCGTAATGAA
AATTCAGCAGGACATATTATTACCGTTGAAGATCCAGTCGAATACGTACATAAACACAAAAAGTCGATGATTACGCATCG
TGAAGTGGGCGTCGATTGTCATTCATGGCATAACGCACTGAAAAACACCTTACGTCAAGCACCTGACGTGATCTTGATTG
GTGAGATTCGCGATACTGAAACCATGGAACATGCAATTGCTTTTGCTGAAACGGGCCATTTATGTTTAGGAACGCTACAC
TCAAATAATGCAAATCAAACTCTTGATCGGATTATTAACTTTTTCCCAGAGGAACGCCGTAATCAATTATTGATGGATTT
ATCATCGAATATGAAAGCGATTATTTCACAGCGTTTAATTCGGACAGAAGATGGCCAAGGGCGTCGTGCTGCTATTGAAA
TTATGCTGAATACCCCCCTTATTTCAGATTTAATTCTCAAAGGCGAATTCCATGAGCTAAAAGGTATTATGTCAAAGTCA
CGTGAATTGGGCATGCAAACCTTTGATCAGGCACTCTTTGATTTATATAACGAAGGTGCAATTTCTTATGAAGAAGCCTT
ACGCAATGCCGATTCGACCAATGAATTAAGGTTGCAGATCAAACTCAAAGGGCAAAGAAGGCATGCTTCGCAAGATGCAG
CAGCGACCGCCTTTACGATGTATGAAGAACCTAAAAAAGAAGAAAACGAAGACGAAAACCAAGAATCTGCTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (118-282)

3D structure

• AlphaFold DB: A0A5Q0P3W7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.561	87.154	0.537
pilU	Acinetobacter baylyi ADP1	55.62	87.406	0.486
pilU	Vibrio cholerae strain A1552	51.549	89.421	0.461
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.984	92.191	0.378
pilT	Pseudomonas aeruginosa PAK	43.865	82.116	0.36