Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GCD22_RS01245 Genome accession   NZ_CP045571
Coordinates   236753..238471 (+) Length   572 a.a.
NCBI ID   WP_024893402.1    Uniprot ID   -
Organism   Acidithiobacillus thiooxidans ATCC 19377     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 231753..243471
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCD22_RS01220 (GCD22_00250) xth 232124..232894 (+) 771 WP_024893407.1 exodeoxyribonuclease III -
  GCD22_RS01225 (GCD22_00251) hemH 232894..233907 (+) 1014 WP_024893406.1 ferrochelatase -
  GCD22_RS01230 (GCD22_00252) - 233963..234445 (+) 483 WP_024893405.1 peptidylprolyl isomerase -
  GCD22_RS01235 (GCD22_00253) - 234502..235635 (+) 1134 WP_024893404.1 anhydro-N-acetylmuramic acid kinase -
  GCD22_RS01240 (GCD22_00254) - 235899..236783 (+) 885 WP_024893403.1 shikimate dehydrogenase -
  GCD22_RS01245 (GCD22_00255) pilB 236753..238471 (+) 1719 WP_024893402.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GCD22_RS01250 (GCD22_00256) - 238504..240117 (+) 1614 WP_024893401.1 ATP-binding protein -
  GCD22_RS01255 (GCD22_00257) pilR 240114..241472 (+) 1359 WP_050816103.1 sigma-54 dependent transcriptional regulator Regulator
  GCD22_RS01260 (GCD22_00258) - 241469..242269 (+) 801 WP_031569873.1 A24 family peptidase -
  GCD22_RS01265 (GCD22_00259) coaE 242276..242884 (+) 609 WP_031569871.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 63021.60 Da        Isoelectric Point: 5.9498

>NTDB_id=395252 GCD22_RS01245 WP_024893402.1 236753..238471(+) (pilB) [Acidithiobacillus thiooxidans ATCC 19377]
MAANPEQITLTPVLRALVSNGLGNEAQLLALASDPGRGKKPLLFYAVEKGAVSAPALMAHLSQRYNMPMLDLDAVMIDDL
LIRRIDKNLMSRYFVLPLSKRGETLYLAMADPTDFKAVEDIKFNTGLQVVPILVEANKLMRAVNLANNAGTGIDDVFLEK
SSDVNTDDAQEEFDLSKDSDGTVDDAPVVRFVQQLLLDAIQRGVSDIHLEPYEKDFRVRYRLDGVLQDALHPPLALRDGV
TSRLKILCRLDISERRLPQDGRLRVRVPPARVIDFRVSFLPTSFGETIVLRLLDPASSRVPIEQLGFLPEQRKAFEEAIH
RPYGMILVTGPTGSGKTTTLYTALNILNTGDCNISTAEDPVEIPVYGINQVNINERIGLNFAAALRSFLRQDPDIIMVGE
VRDLETAETAIKAAQTGHLVLATLHTNDAPQSLTRLENMGIPTYNIAGGVHLVMAQRLARKLCTHCKKPVRIPDQALVEA
GFAADDLQGWRPMGPTGCDECNKTGYKGRTGLYQVMPVSEAMREIIMRGGTAIDLARQAASEGVLTMRQNGLRRIKEGIT
SLEEVLRVTNLQ

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=395252 GCD22_RS01245 WP_024893402.1 236753..238471(+) (pilB) [Acidithiobacillus thiooxidans ATCC 19377]
ATGGCTGCAAACCCGGAACAAATCACTTTAACGCCCGTATTGCGGGCACTGGTCAGCAATGGGTTGGGTAATGAGGCGCA
ATTGCTTGCCCTGGCTAGTGATCCCGGGCGGGGAAAAAAACCATTACTGTTTTATGCAGTAGAAAAAGGGGCTGTTTCGG
CACCTGCGCTGATGGCGCATTTATCCCAGCGCTACAATATGCCAATGCTTGATCTGGATGCGGTCATGATTGATGACCTG
TTGATACGCCGGATTGATAAAAATCTGATGAGCCGTTATTTCGTTCTGCCCTTGTCCAAACGCGGCGAGACACTTTATCT
GGCGATGGCAGATCCTACCGACTTCAAAGCCGTTGAGGACATCAAGTTCAATACCGGCCTGCAAGTTGTGCCGATACTTG
TGGAAGCCAACAAGCTGATGCGCGCGGTAAACCTGGCCAATAATGCCGGAACAGGAATTGATGATGTCTTCCTTGAAAAG
TCATCAGATGTAAATACCGATGATGCCCAGGAAGAATTTGATTTATCCAAGGATAGCGATGGCACTGTGGATGATGCACC
GGTGGTGCGTTTTGTGCAGCAGCTGCTTTTGGATGCGATTCAGCGCGGCGTATCGGATATTCATCTGGAACCCTATGAGA
AGGATTTCCGGGTGCGTTACCGCCTGGATGGGGTGTTGCAGGATGCTCTGCATCCGCCACTGGCCTTGCGGGATGGGGTC
ACGTCGCGGCTGAAAATTCTCTGTCGCCTGGATATTTCGGAGCGGCGTTTGCCTCAAGATGGACGCTTGCGGGTGCGTGT
GCCTCCGGCTCGGGTCATTGATTTTCGTGTGTCTTTCCTGCCAACAAGTTTCGGGGAAACCATTGTCCTGCGTCTGCTGG
ATCCTGCCAGTTCCAGGGTACCCATCGAGCAACTGGGTTTTTTACCGGAACAACGCAAAGCCTTTGAAGAGGCCATTCAC
CGTCCCTATGGCATGATTCTGGTGACCGGACCTACCGGCTCCGGTAAAACCACGACGCTATATACTGCATTGAACATTCT
CAATACGGGTGACTGTAATATCAGCACGGCGGAAGATCCGGTAGAAATTCCGGTGTATGGAATCAATCAGGTGAATATCA
ATGAGCGGATCGGCTTGAATTTTGCCGCTGCCCTGCGGTCATTTTTACGTCAGGATCCCGATATTATCATGGTCGGTGAA
GTCCGCGATCTGGAAACCGCAGAAACTGCGATAAAAGCTGCCCAGACCGGACATTTGGTGTTGGCCACGCTCCATACCAA
TGATGCACCTCAGAGCCTGACCCGTCTGGAGAACATGGGTATTCCTACCTACAATATCGCCGGTGGAGTGCACCTGGTCA
TGGCCCAACGTCTGGCCCGAAAGCTGTGTACGCATTGCAAAAAGCCGGTTCGTATTCCTGATCAGGCGCTTGTGGAAGCC
GGGTTTGCTGCGGATGATCTGCAAGGCTGGCGTCCCATGGGTCCTACGGGTTGTGATGAATGTAACAAAACGGGCTATAA
AGGGCGTACGGGTCTCTATCAGGTCATGCCAGTCAGTGAAGCGATGCGCGAAATCATCATGCGGGGTGGCACCGCTATAG
ATTTGGCACGACAGGCAGCCAGTGAGGGGGTGCTCACCATGCGCCAGAATGGTCTACGCAGAATCAAGGAAGGCATAACC
AGTCTGGAAGAAGTTTTGCGGGTTACCAATCTGCAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

48.962

100

0.495
  pilB Acinetobacter baumannii D1279779

47.735

100

0.479
  pilB Acinetobacter baylyi ADP1

47.359

99.301

0.47
  pilF Neisseria gonorrhoeae MS11

45.293

98.427

0.446
  pilB Vibrio cholerae strain A1552

46.792

92.657

0.434
  pilB Vibrio parahaemolyticus RIMD 2210633

46.226

92.657

0.428
  pilB Vibrio campbellii strain DS40M4

45.351

92.133

0.418
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.267

91.608

0.369
  pilF Thermus thermophilus HB27

39.811

92.657

0.369

Multiple sequence alignment