Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FM038_RS01915 Genome accession   NZ_CP045503
Coordinates   439573..441282 (-) Length   569 a.a.
NCBI ID   WP_142871699.1    Uniprot ID   A0AAJ4AR70
Organism   Shewanella eurypsychrophilus strain YLB-08     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 434573..446282
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FM038_RS01885 (FM038_001900) mutT 435138..435545 (-) 408 WP_142871693.1 8-oxo-dGTP diphosphatase MutT -
  FM038_RS01890 (FM038_25300) yacG 435549..435761 (-) 213 WP_142871694.1 DNA gyrase inhibitor YacG -
  FM038_RS01895 (FM038_001905) zapD 435847..436581 (-) 735 WP_142871695.1 cell division protein ZapD -
  FM038_RS01900 (FM038_001910) coaE 436590..437195 (-) 606 WP_142871696.1 dephospho-CoA kinase -
  FM038_RS01905 (FM038_001915) pilD 437231..438142 (-) 912 WP_142871697.1 prepilin peptidase Machinery gene
  FM038_RS01910 (FM038_001920) pilC 438202..439473 (-) 1272 WP_142871698.1 type II secretion system F family protein Machinery gene
  FM038_RS01915 (FM038_001925) pilB 439573..441282 (-) 1710 WP_142871699.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FM038_RS01920 (FM038_001930) - 441361..441795 (-) 435 WP_142871700.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FM038_RS01925 (FM038_001935) nadC 442716..443618 (-) 903 WP_142871701.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FM038_RS01930 (FM038_001940) ampD 443968..444531 (+) 564 WP_142871702.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FM038_RS01935 (FM038_001945) ampE 444550..445401 (+) 852 WP_142871703.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63114.26 Da        Isoelectric Point: 5.0081

>NTDB_id=394637 FM038_RS01915 WP_142871699.1 439573..441282(-) (pilB) [Shewanella eurypsychrophilus strain YLB-08]
MPTTGLHLGLSTLFIRKNLLSEEQIASAIAKSRQNKQSLVSTIVSEKLISAREIAELCYEEYGTPLLDLDEFDIASIPEE
FLNKKLIDKHKCLPLFKRGNRLYIGTSDPTNIAALEDFQFSAGLHAEAILVEDDKLSKAIEKTLEEDISALDLDGIDEAA
LADIEVDDPSKREEEQKGDGSDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPVNLTNRISA
RLKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTIWGEKIVMRILDSSSAQLGIEKLGYEDDQRELYEEMLEKP
QGMILVTGPTGSGKTVSLYTGLNILNTEARNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVAGYNIASSVNLIIAQRLARRLCPECKQPEEIPEHELQRLGF
KQASIDSGITTYKPVGCDLCSGGYKGRVGIYEVMRMSDEIARTIMEGGNSLMIATQAKEQGMRDLRESGLLKVIKGVTSI
TEINRVTSF

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=394637 FM038_RS01915 WP_142871699.1 439573..441282(-) (pilB) [Shewanella eurypsychrophilus strain YLB-08]
ATGCCAACTACGGGCCTCCACTTAGGCTTATCAACACTGTTCATCCGTAAGAATTTGCTCAGCGAAGAGCAGATCGCTTC
CGCTATAGCCAAATCTCGTCAGAATAAACAATCTCTCGTCTCCACCATAGTCTCAGAAAAACTTATCTCTGCTCGCGAGA
TAGCCGAGCTGTGTTATGAAGAATATGGCACCCCACTGCTCGATCTCGATGAATTTGACATAGCCAGCATCCCCGAAGAG
TTTCTCAATAAAAAGCTCATCGATAAACATAAATGTTTGCCGCTGTTTAAACGTGGCAACCGCTTGTACATAGGTACATC
TGACCCGACTAATATTGCAGCCTTAGAAGATTTTCAATTCAGCGCTGGCCTACATGCCGAAGCGATTTTGGTCGAAGATG
ATAAACTCAGTAAGGCCATCGAGAAGACCCTCGAAGAGGATATCTCAGCGCTGGATCTCGATGGCATAGACGAAGCGGCA
CTGGCCGATATAGAAGTCGATGATCCAAGTAAACGAGAGGAAGAGCAAAAAGGGGATGGCAGCGATGATGCCCCGATTGT
CATCTATATCAATAAAATTTTGACCGATGCCATTCGCAAAGGTGCCTCGGATCTGCATTTCGAGCCTTATGAGAAACGCT
ACCGTATCCGTTTTCGCATCGATGGCATCTTACATGAAGTCTCAGAGCCTCCTGTAAATTTAACTAATCGTATCTCAGCT
CGCTTAAAGGTGATGTCTAAACTCGATATCGCCGAACGAAGAGTCCCGCAAGATGGCCGCATCAAGATGAAGCTATCTCG
CACTAAGTCCATCGATTTTCGTGTCAGCACCCTGCCTACGATCTGGGGCGAGAAGATAGTAATGCGTATCTTAGACTCCT
CCTCTGCCCAATTAGGTATCGAGAAACTAGGTTATGAGGATGATCAGCGCGAATTGTACGAGGAGATGCTAGAAAAACCC
CAGGGCATGATACTGGTAACTGGCCCCACAGGCTCAGGTAAAACGGTTTCCCTGTATACAGGGCTCAACATTCTCAATAC
CGAAGCGCGCAATATATCCACTGCCGAAGACCCTGTAGAGATAAACCTCGAGGGTGTTAACCAAGTTCATATTAATCTCA
AAGCTGGCTTAACATTCGCCTCGGCATTGCGCTCTTTCCTACGACAAGATCCCGATGTGGTGATGGTCGGTGAGATCCGT
GATTTAGAAACGGCAGAAATCGCCATCAAGGCCGCTCAAACGGGTCACTTGGTGCTATCTACCTTACATACCAACTCTGC
AGCAGAAACCTTAACGCGTCTTATCAATATGGGGGTGGCTGGCTATAATATAGCGAGCTCAGTCAACCTCATCATTGCAC
AACGACTCGCGCGTCGCCTCTGCCCTGAGTGTAAACAACCCGAAGAAATTCCAGAACATGAACTGCAAAGGTTGGGTTTT
AAGCAAGCTAGCATTGATAGTGGGATCACTACCTACAAACCTGTGGGCTGCGATCTCTGCTCTGGCGGCTATAAAGGCCG
TGTGGGCATTTATGAAGTCATGAGAATGTCAGATGAAATTGCCCGTACTATTATGGAGGGAGGCAACTCATTGATGATAG
CCACACAAGCCAAAGAGCAGGGCATGCGAGATTTAAGAGAATCAGGGTTATTAAAGGTCATCAAAGGCGTCACCAGTATC
ACAGAGATAAATCGAGTCACTAGTTTCTAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(32-143)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.932

98.594

0.571
  pilB Acinetobacter baumannii D1279779

57.194

98.946

0.566
  pilB Legionella pneumophila strain ERS1305867

55.654

99.473

0.554
  pilB Vibrio parahaemolyticus RIMD 2210633

51.839

100

0.52
  pilB Vibrio cholerae strain A1552

51.773

99.121

0.513
  pilB Vibrio campbellii strain DS40M4

50.796

99.297

0.504
  pilF Neisseria gonorrhoeae MS11

47.183

99.824

0.471
  pilF Thermus thermophilus HB27

38.434

98.77

0.38
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.779

97.891

0.38

Multiple sequence alignment