Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FIU83_RS07445 Genome accession   NZ_CP045417
Coordinates   1637550..1639346 (-) Length   598 a.a.
NCBI ID   WP_152483466.1    Uniprot ID   A0A5P9ILW5
Organism   Halomonas sp. THAF5a     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1632550..1644346
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIU83_RS07420 (FIU83_07420) dinB 1632753..1633799 (-) 1047 WP_152485288.1 DNA polymerase IV -
  FIU83_RS07425 (FIU83_07425) lexA 1633860..1634519 (-) 660 WP_152483462.1 transcriptional repressor LexA -
  FIU83_RS07430 (FIU83_07430) - 1634923..1635591 (+) 669 WP_152483463.1 TetR/AcrR family transcriptional regulator -
  FIU83_RS07435 (FIU83_07435) - 1635591..1636109 (+) 519 WP_152483464.1 L,D-transpeptidase -
  FIU83_RS07440 (FIU83_07440) pilC 1636252..1637487 (-) 1236 WP_152483465.1 type II secretion system F family protein Machinery gene
  FIU83_RS07445 (FIU83_07445) pilB 1637550..1639346 (-) 1797 WP_152483466.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FIU83_RS17760 - 1639745..1640092 (+) 348 WP_152483467.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FIU83_RS07455 - 1640409..1641262 (+) 854 WP_152483468.1 IS5 family transposase -
  FIU83_RS07460 (FIU83_07460) istA 1641618..1642372 (+) 755 Protein_1467 IS21 family transposase -
  FIU83_RS17440 (FIU83_07465) - 1642471..1642665 (+) 195 Protein_1468 transposase -
  FIU83_RS07470 (FIU83_07470) - 1642708..1643910 (-) 1203 WP_152483471.1 ISL3 family transposase -

Sequence

Protein


Download         Length: 598 a.a.        Molecular weight: 64853.28 Da        Isoelectric Point: 5.5215

>NTDB_id=393879 FIU83_RS07445 WP_152483466.1 1637550..1639346(-) (pilB) [Halomonas sp. THAF5a]
MNDYRPPSPEQPPHVPTPEPSALQGATLRGGLRGLARRVVADGLLNEAAVVRAEAEAREADRSLLAHLIEVGMVPARQAT
LSAAWEYGLPIIDLDALRLESLPPARDYPDKILRRLGVLPLVHHGHRLTVAVPYPSTLALLDELQFATGLTLEGVLAPVD
QLGPVLEGYLASSETSMFDELAGVDDAVGELAYDEGVVDATDEQAAVAAAGDDAPIVKFVNKILLDAIQRGASDIHFEPY
ETSYRVRFRIDGILHDVAHPPFAMRSRISARLKVMSRLDISERRLPQDGAIKLKLSTTRSIDFRVNSLPTVFGEKVVLRI
LDPSSAQLGIEQLGFTPPQRALYERVLGEPQGMILVTGPTGSGKTVSLYTGINILNQVERNICTAEDPVEIKVPGVNQVN
VLPKIGLTFASALRAFLRQDPDVVMVGEIRDLETAEIAVKASQTGHLVLSTVHTNSAAETLTRLANMGVAPFNIASSVSL
IIAQRLARRLCQRCKQPAEIPRESLLAQGFTEDEVASATIFEPVGCQHCTQGFKGRVGIYEVVPISDAMSHLIMTQGNSR
DFDAQARQEGHPDLRRSGILKVMQGLTSLAEVNRVTKD

Nucleotide


Download         Length: 1797 bp        

>NTDB_id=393879 FIU83_RS07445 WP_152483466.1 1637550..1639346(-) (pilB) [Halomonas sp. THAF5a]
ATGAACGACTACCGTCCCCCGTCCCCCGAGCAGCCCCCCCACGTGCCGACGCCCGAGCCGTCGGCGCTGCAGGGGGCCAC
CCTGCGCGGCGGGCTGCGCGGCCTGGCGCGCCGAGTGGTCGCTGACGGCCTGCTGAATGAGGCAGCGGTGGTGCGCGCAG
AAGCCGAGGCTCGCGAGGCGGACCGCTCTCTGCTCGCTCACCTGATCGAGGTGGGCATGGTGCCCGCCCGCCAGGCCACG
CTAAGCGCGGCCTGGGAGTATGGACTGCCGATCATCGACCTCGATGCCCTGCGCCTGGAGAGCCTGCCACCGGCCAGGGA
CTACCCGGACAAGATCCTGCGCCGGCTTGGGGTGCTGCCGCTGGTGCACCACGGCCACCGGCTCACGGTGGCGGTGCCCT
ACCCCTCCACCCTGGCGCTGCTCGACGAGCTGCAGTTCGCCACCGGCCTGACCCTGGAGGGCGTGTTGGCACCGGTGGAC
CAGCTCGGCCCGGTGCTCGAAGGTTATCTCGCCAGCAGCGAGACCAGCATGTTCGATGAACTCGCCGGCGTGGACGACGC
CGTGGGGGAGCTCGCCTACGACGAAGGGGTCGTCGATGCCACGGACGAGCAGGCCGCGGTGGCCGCCGCGGGTGACGACG
CACCCATCGTCAAGTTCGTCAACAAGATCCTGCTGGACGCGATCCAGCGGGGCGCCTCGGATATCCACTTCGAGCCGTAC
GAGACTAGCTATCGGGTGCGCTTTCGCATCGACGGCATCCTCCACGACGTGGCACACCCGCCCTTTGCCATGCGCTCGCG
CATCTCGGCGCGCTTGAAGGTGATGTCGCGCCTGGATATCTCCGAACGCCGCCTGCCCCAGGACGGTGCCATCAAGCTCA
AGCTCTCGACGACGCGCTCCATCGATTTTCGCGTCAACTCCCTGCCCACCGTCTTCGGCGAGAAGGTGGTGCTGCGGATT
CTCGACCCGAGCTCGGCGCAGCTCGGCATCGAGCAGCTTGGCTTCACCCCGCCACAGCGAGCCCTCTATGAGCGCGTGCT
CGGCGAGCCCCAGGGGATGATCCTGGTCACCGGCCCCACCGGCAGCGGCAAGACGGTCTCGCTCTATACCGGTATCAACA
TTCTCAACCAGGTGGAGCGCAACATCTGCACCGCCGAGGATCCCGTCGAGATCAAGGTGCCGGGCGTCAACCAGGTCAAC
GTCCTGCCCAAGATCGGCCTGACCTTCGCCAGCGCCCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGGTGATGGTCGG
CGAGATCCGTGACCTGGAGACCGCCGAGATCGCGGTCAAGGCCTCCCAGACCGGCCACCTGGTGCTCTCCACAGTGCACA
CCAACTCGGCGGCGGAGACACTGACCCGCCTGGCCAACATGGGCGTGGCACCCTTCAATATCGCGAGTTCCGTGAGCCTG
ATCATCGCCCAGCGCCTGGCGCGCCGGCTCTGCCAGCGCTGCAAGCAGCCCGCCGAGATCCCCCGCGAATCGCTGCTCGC
CCAGGGCTTCACCGAGGATGAGGTGGCGAGCGCCACCATCTTTGAACCGGTGGGCTGCCAGCACTGCACCCAGGGCTTCA
AGGGTCGGGTGGGCATCTACGAGGTCGTACCGATCTCTGATGCCATGAGCCACCTGATCATGACTCAGGGCAATTCCCGT
GACTTCGACGCCCAGGCCCGCCAGGAAGGCCATCCGGATCTCCGTCGCAGCGGCATCCTCAAGGTGATGCAGGGGCTGAC
CAGCCTGGCAGAGGTCAATCGAGTAACGAAGGACTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(68-174)

Type II/IV secretion system protein (T2SSE)

(222-489)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5P9ILW5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.641

95.485

0.522
  pilB Acinetobacter baylyi ADP1

52.972

95.652

0.507
  pilB Legionella pneumophila strain ERS1305867

52.373

95.151

0.498
  pilB Vibrio campbellii strain DS40M4

51.301

89.967

0.462
  pilB Vibrio cholerae strain A1552

49.907

89.465

0.446
  pilF Neisseria gonorrhoeae MS11

47.482

92.977

0.441
  pilB Vibrio parahaemolyticus RIMD 2210633

53.157

82.107

0.436
  pilB/pilB1 Synechocystis sp. PCC 6803

36.834

100

0.381

Multiple sequence alignment