Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FIU88_RS09845 Genome accession   NZ_CP045399
Coordinates   2144703..2146496 (+) Length   597 a.a.
NCBI ID   WP_152479098.1    Uniprot ID   A0A5P9HHB3
Organism   Halomonas sp. THAF12     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2139703..2151496
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIU88_RS09825 (FIU88_09860) - 2139786..2140391 (+) 606 WP_172977700.1 YjbF family lipoprotein -
  FIU88_RS09830 (FIU88_09865) - 2140528..2141217 (+) 690 WP_172977701.1 capsule biosynthesis GfcC family protein -
  FIU88_RS09835 (FIU88_09870) - 2141298..2143316 (+) 2019 WP_216647269.1 YjbH domain-containing protein -
  FIU88_RS19515 - 2143851..2144414 (-) 564 WP_301339534.1 pilin -
  FIU88_RS09845 (FIU88_09875) pilB 2144703..2146496 (+) 1794 WP_152479098.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FIU88_RS09850 (FIU88_09880) pilC 2146521..2147753 (+) 1233 WP_152479099.1 type II secretion system F family protein Machinery gene
  FIU88_RS09860 (FIU88_09885) - 2147948..2148592 (-) 645 WP_016856438.1 TetR/AcrR family transcriptional regulator -
  FIU88_RS09865 (FIU88_09890) lexA 2148836..2149498 (+) 663 WP_016856439.1 transcriptional repressor LexA -
  FIU88_RS09870 (FIU88_09895) dinB 2149543..2150598 (+) 1056 WP_152479101.1 DNA polymerase IV -

Sequence

Protein


Download         Length: 597 a.a.        Molecular weight: 65371.60 Da        Isoelectric Point: 5.3069

>NTDB_id=393765 FIU88_RS09845 WP_152479098.1 2144703..2146496(+) (pilB) [Halomonas sp. THAF12]
MNDYRPPSADRPPSSHQADTSPAGRLRGMRGLARRLVTDGLLTEAAAQLAEQEAREAETSLLKHVITTGLVQARQATLTA
AWEYGLPIIDLDALRLESLPDAGDYPDKLLRRLGVLPLHRRGHKLTVAVPYPSTLAQLDELQFATGLSLEGVLAPIDQLG
PVLEAYLARSESSMLDELSNVDDAVGELEFGEDVVDVTNEQQAIANANAGDDTPIVKFVNKILLDAIRRGASDIHFEPYE
TSYRVRFRIDGILHDAAHPPFAMRNRIAARLKVMARLDISERRLPQDGAIKLKVSSTRSIDFRVNSLPTVYGEKMVLRIL
DPSSAQMGIEQLGFTPEQRAMYENVLEHPQGMILVTGPTGSGKTVTLYTGINILNEIERNICTAEDPVEIRVPGVNQVNV
MPRIGLDFASALRAFLRQDPDVVMVGEIRDLETAEIAVKASQTGHLVLSTVHTNSAAETLTRLANMGVAPFNIASSVSLI
IAQRLARRLCPNCKQPADIPREALLAQGFSEEEVEGATIYEPVGCQHCTQGFKGRVGIYEVVPISHAMSQLIMTHGNAMD
FDTQAREEGHPDLRRSGLLKVMQGMTSLAEVNRVTKE

Nucleotide


Download         Length: 1794 bp        

>NTDB_id=393765 FIU88_RS09845 WP_152479098.1 2144703..2146496(+) (pilB) [Halomonas sp. THAF12]
ATGAACGACTATCGTCCTCCCTCTGCCGATCGCCCGCCTTCCTCACACCAGGCGGACACCTCACCCGCCGGCCGCCTGCG
CGGCATGCGCGGCCTGGCCCGCCGGCTGGTGACGGACGGCCTGCTGACCGAGGCGGCGGCACAGCTCGCCGAGCAGGAGG
CCCGGGAGGCCGAGACCTCGCTGCTCAAGCACGTCATCACCACGGGGCTGGTGCAGGCGCGTCAGGCCACGCTCACGGCC
GCCTGGGAATATGGCCTGCCGATCATCGATCTGGATGCCCTGCGGCTGGAGAGCCTGCCCGACGCCGGCGACTATCCCGA
CAAGCTGCTGCGCAGGCTCGGCGTGCTGCCGCTGCACCGCCGCGGCCACAAGCTCACTGTCGCCGTGCCCTACCCCAGCA
CCCTGGCCCAGCTCGACGAACTGCAGTTCGCCACCGGCCTGTCGCTGGAGGGAGTGCTGGCCCCAATCGATCAGCTCGGC
CCGGTGCTGGAGGCCTACCTGGCCCGCAGCGAGTCGTCGATGCTCGACGAGCTGTCCAACGTCGACGACGCCGTGGGCGA
GCTGGAGTTCGGTGAGGACGTCGTCGACGTCACCAACGAACAGCAGGCGATCGCCAACGCCAACGCCGGCGACGATACGC
CGATCGTCAAGTTCGTCAACAAGATCCTGCTCGACGCCATCCGCCGCGGCGCCTCGGACATCCACTTCGAACCTTACGAG
ACCAGCTACCGGGTGCGCTTTCGCATCGACGGCATCCTGCACGATGCGGCCCATCCGCCCTTCGCCATGCGCAATCGCAT
CGCCGCCCGCCTCAAGGTCATGGCGCGGCTGGACATCTCCGAGCGCCGCCTGCCCCAGGACGGCGCCATCAAGCTCAAGG
TCTCCTCGACCCGCTCCATCGACTTTCGCGTCAACTCGCTGCCCACCGTCTACGGCGAGAAGATGGTCCTGCGTATCCTC
GATCCCAGCTCGGCACAGATGGGCATCGAGCAGCTGGGCTTCACGCCCGAGCAGCGCGCCATGTACGAGAACGTGCTGGA
GCACCCTCAGGGCATGATCCTGGTCACCGGCCCCACCGGCAGCGGCAAGACGGTGACGCTCTACACCGGCATCAACATCC
TCAACGAGATCGAGCGCAACATCTGCACCGCCGAGGACCCGGTGGAGATCAGGGTGCCCGGCGTCAACCAGGTCAACGTG
ATGCCGCGCATCGGGCTCGACTTCGCCAGCGCCCTGCGCGCCTTCCTGCGCCAGGACCCGGATGTGGTGATGGTCGGCGA
GATCCGCGACCTGGAAACCGCCGAGATCGCGGTCAAGGCCTCCCAGACCGGCCACCTGGTGCTCTCCACCGTGCACACCA
ACTCCGCGGCGGAGACCCTCACCCGCTTGGCCAACATGGGCGTGGCGCCGTTCAATATCGCGAGTTCCGTGAGCCTGATC
ATCGCCCAGCGCCTGGCCCGGCGGCTGTGCCCGAACTGCAAGCAGCCCGCCGACATTCCGCGCGAGGCGCTGCTGGCCCA
GGGCTTCAGCGAGGAAGAGGTCGAGGGCGCCACCATCTACGAACCGGTGGGCTGCCAGCACTGCACCCAGGGCTTCAAGG
GCCGGGTCGGCATCTACGAGGTGGTGCCGATCTCCCACGCCATGAGCCAGCTGATCATGACCCACGGCAACGCCATGGAC
TTCGATACCCAGGCGCGCGAGGAAGGCCACCCGGACCTGCGCCGCAGCGGCCTGTTGAAGGTCATGCAGGGCATGACCAG
CCTGGCCGAGGTCAACCGGGTCACCAAGGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-170)

Type II/IV secretion system protein (T2SSE)

(221-488)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5P9HHB3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

52.715

95.645

0.504
  pilB Legionella pneumophila strain ERS1305867

52.724

95.31

0.503
  pilB Acinetobacter baylyi ADP1

51.309

95.98

0.492
  pilB Vibrio campbellii strain DS40M4

48.214

93.802

0.452
  pilB Vibrio cholerae strain A1552

49.442

90.117

0.446
  pilB Vibrio parahaemolyticus RIMD 2210633

46.82

94.807

0.444
  pilF Neisseria gonorrhoeae MS11

45.455

95.812

0.436
  pilB/pilB1 Synechocystis sp. PCC 6803

40.797

88.275

0.36

Multiple sequence alignment