Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   HPT09_RS22580 Genome accession   NZ_CP053706
Coordinates   4889497..4890120 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain PAC1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4884497..4895120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPT09_RS22560 (HPT09_22565) aroB 4885092..4886198 (-) 1107 WP_003114574.1 3-dehydroquinate synthase -
  HPT09_RS22565 (HPT09_22570) aroK 4886248..4886766 (-) 519 WP_003095833.1 shikimate kinase AroK -
  HPT09_RS22570 (HPT09_22575) pilQ 4886778..4888922 (-) 2145 WP_003114575.1 type 4a pilus secretin PilQ Machinery gene
  HPT09_RS22575 (HPT09_22580) comL/pilP 4888976..4889500 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  HPT09_RS22580 (HPT09_22585) pilO 4889497..4890120 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  HPT09_RS22585 (HPT09_22590) pilN 4890117..4890713 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  HPT09_RS22590 (HPT09_22595) pilM 4890713..4891777 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  HPT09_RS22595 (HPT09_22600) - 4891962..4894433 (+) 2472 WP_003095841.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=393572 HPT09_RS22580 WP_003103268.1 4889497..4890120(-) (pilO) [Pseudomonas aeruginosa strain PAC1]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=393572 HPT09_RS22580 WP_003103268.1 4889497..4890120(-) (pilO) [Pseudomonas aeruginosa strain PAC1]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401