Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GB851_RS05355 Genome accession   NZ_CP045141
Coordinates   1026583..1027767 (+) Length   394 a.a.
NCBI ID   WP_003790159.1    Uniprot ID   -
Organism   Kingella kingae strain F41215CHC     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1008393..1030304 1026583..1027767 within 0


Gene organization within MGE regions


Location: 1008393..1030304
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GB851_RS05235 (GB851_05390) - 1008456..1008836 (+) 381 WP_164538157.1 hypothetical protein -
  GB851_RS05240 (GB851_05395) - 1008957..1009400 (-) 444 WP_003790578.1 phage virion morphogenesis protein -
  GB851_RS05245 (GB851_05400) - 1009402..1010313 (-) 912 Protein_1030 phage minor head protein -
  GB851_RS05250 (GB851_05405) - 1010318..1010527 (-) 210 WP_003786202.1 DUF935 family protein -
  GB851_RS05255 (GB851_05410) - 1010579..1011094 (+) 516 WP_003792210.1 phage major tail tube protein -
  GB851_RS05260 (GB851_05415) - 1011232..1011519 (+) 288 WP_003792208.1 phage tail assembly protein -
  GB851_RS05265 (GB851_05420) - 1011572..1011685 (+) 114 WP_003792207.1 GpE family phage tail protein -
  GB851_RS05270 (GB851_05425) - 1011688..1011876 (-) 189 WP_164538113.1 hypothetical protein -
  GB851_RS05275 (GB851_05430) - 1011925..1012146 (+) 222 WP_003792203.1 hypothetical protein -
  GB851_RS05280 (GB851_05435) - 1012369..1013235 (+) 867 WP_003792202.1 phage tail tape measure protein -
  GB851_RS05285 (GB851_05440) - 1013228..1013755 (+) 528 WP_003792200.1 hypothetical protein -
  GB851_RS05290 (GB851_05445) - 1013897..1014334 (+) 438 WP_164538158.1 DNA adenine methylase -
  GB851_RS05295 (GB851_05450) - 1014598..1015233 (+) 636 Protein_1040 KpsF/GutQ family sugar-phosphate isomerase -
  GB851_RS05300 (GB851_05455) - 1015313..1016926 (-) 1614 WP_038310639.1 phosphoethanolamine transferase -
  GB851_RS05305 (GB851_05460) - 1017164..1017868 (-) 705 WP_164538159.1 IS1 family transposase -
  GB851_RS05310 (GB851_05465) - 1018122..1018316 (-) 195 WP_338153837.1 DUF1330 domain-containing protein -
  GB851_RS05315 (GB851_05470) thrS 1018495..1020408 (-) 1914 WP_038310880.1 threonine--tRNA ligase -
  GB851_RS05320 (GB851_05475) - 1020588..1020758 (-) 171 WP_003786175.1 zf-HC2 domain-containing protein -
  GB851_RS05325 (GB851_05480) - 1020755..1021348 (-) 594 WP_164538160.1 sigma-70 family RNA polymerase sigma factor -
  GB851_RS05330 - 1021462..1021635 (-) 174 WP_003786173.1 hypothetical protein -
  GB851_RS05335 (GB851_05485) - 1021891..1023342 (-) 1452 WP_003790163.1 sodium:proton antiporter -
  GB851_RS05340 (GB851_05490) - 1023465..1024310 (-) 846 WP_003786171.1 PHP domain-containing protein -
  GB851_RS05345 (GB851_05495) - 1024473..1025813 (-) 1341 WP_019389073.1 DUF3482 domain-containing protein -
  GB851_RS05350 (GB851_05500) - 1025919..1026401 (-) 483 WP_019389074.1 Lrp/AsnC family transcriptional regulator -
  GB851_RS05355 (GB851_05505) pilU 1026583..1027767 (+) 1185 WP_003790159.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GB851_RS05360 (GB851_05510) - 1027850..1028605 (+) 756 WP_003790157.1 sulfite exporter TauE/SafE family protein -
  GB851_RS05365 (GB851_05515) - 1028620..1029150 (+) 531 WP_003790154.1 CreA family protein -
  GB851_RS05370 (GB851_05520) hisI 1029160..1029555 (+) 396 WP_164538161.1 phosphoribosyl-AMP cyclohydrolase -
  GB851_RS05375 (GB851_05525) - 1029583..1029912 (+) 330 WP_003790151.1 phosphoribosyl-ATP diphosphatase -
  GB851_RS05380 (GB851_05530) - 1029984..1030304 (+) 321 WP_003790149.1 histidine triad nucleotide-binding protein -

Sequence

Protein


Download         Length: 394 a.a.        Molecular weight: 43718.21 Da        Isoelectric Point: 6.0102

>NTDB_id=392281 GB851_RS05355 WP_003790159.1 1026583..1027767(+) (pilU) [Kingella kingae strain F41215CHC]
MQVIIPPINEEMRKFIHGLLTHMVNNKGSDLFITAGYPPAMKLDGKLTKITDKPLTADHAMQIARSIMDDKQAEEFLTTN
ECNFAISLSGVSRFRINAMVQRGAAALVCRVITSDIPKFDNMNLPPVLKQVVMEKRGLVIFVGGTGSGKSTSLAAMIDYR
NENSYGHIITIEDPIEFVHPHKNCIITQREVGVDTENWFAALKNTLRQAPDVILIGEIRDRETMDYALAFAETGHLCMAT
LHANNSNQALDRIINFFPEERRTQLLNDLSLNLKGFISQRLVPKKQGKGRVAAVEILLNSPLISELVLHGDIHAVKEIMA
KSRDIGMQTFDQSLFDLYEADLISYDDALRNADSVNDLRLQIQLNSKKNATAGSGGVLDGLSLTDYAPPEAEEK

Nucleotide


Download         Length: 1185 bp        

>NTDB_id=392281 GB851_RS05355 WP_003790159.1 1026583..1027767(+) (pilU) [Kingella kingae strain F41215CHC]
ATGCAAGTTATCATTCCACCCATCAACGAAGAAATGCGTAAATTTATCCATGGCTTGCTCACGCACATGGTCAATAACAA
AGGCTCGGATTTATTTATCACGGCTGGCTATCCACCTGCGATGAAATTGGACGGCAAATTAACCAAAATCACCGACAAGC
CTTTAACCGCCGACCACGCCATGCAAATTGCGCGTTCGATTATGGACGACAAGCAAGCCGAAGAATTTTTAACCACCAAC
GAATGCAATTTTGCGATTAGCTTGTCAGGCGTGTCGCGCTTTCGTATCAACGCCATGGTGCAACGTGGTGCAGCAGCTTT
GGTTTGCCGTGTGATTACCAGCGACATTCCCAAATTCGACAACATGAACTTACCGCCTGTGTTAAAACAAGTCGTGATGG
AAAAACGTGGTTTGGTGATTTTTGTCGGCGGTACAGGTTCGGGTAAATCCACATCGTTGGCGGCGATGATTGATTACCGC
AACGAAAATAGCTACGGTCATATCATCACGATTGAAGACCCGATTGAATTTGTTCACCCACACAAAAACTGCATCATCAC
GCAACGCGAAGTGGGTGTGGACACCGAAAACTGGTTCGCCGCGCTCAAAAACACACTGCGCCAAGCACCCGATGTGATTT
TGATTGGTGAGATTCGTGACCGCGAAACGATGGACTACGCACTTGCCTTTGCCGAAACAGGGCACTTGTGTATGGCAACA
CTGCACGCGAATAACTCCAACCAGGCCCTTGACCGCATTATTAACTTCTTCCCCGAAGAACGCCGTACCCAGTTGCTGAA
CGACTTGTCGCTCAACTTAAAAGGCTTTATTTCGCAACGCCTTGTCCCCAAAAAACAAGGCAAAGGACGTGTGGCTGCGG
TAGAAATCCTGCTCAATTCGCCGTTGATTTCCGAATTGGTGTTGCATGGCGACATTCATGCGGTAAAAGAAATTATGGCA
AAATCGCGCGATATTGGTATGCAAACGTTCGACCAATCGCTGTTTGATTTGTATGAAGCCGATTTGATTTCGTATGACGA
TGCCTTGCGTAATGCTGACTCGGTAAACGATTTGCGCCTGCAAATTCAGTTAAACAGCAAGAAAAACGCAACGGCGGGTT
CGGGTGGCGTGCTGGACGGCTTGTCTTTGACGGATTACGCACCGCCAGAAGCCGAAGAAAAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(92-283)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.523

89.34

0.523
  pilU Acinetobacter baylyi ADP1

58.239

89.34

0.52
  pilU Vibrio cholerae strain A1552

53.406

93.147

0.497
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.162

93.909

0.396
  pilT Legionella pneumophila strain Lp02

43.544

84.518

0.368
  pilT Legionella pneumophila strain ERS1305867

43.544

84.518

0.368

Multiple sequence alignment