Detailed information NTG0392157

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: GA721_RS01270
• Genome accession: NZ_CP045131
• Coordinates: 275977..277035 (+)
• Length: 352 a.a.
• NCBI ID: WP_075174946.1
• Uniprot ID: A0A6C0XYY7
• Organism: Acinetobacter indicus strain TQ18
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 270977..282035

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GA721_RS01255	mraY	271119..272237 (+)	1119	WP_005180530.1	phospho-N-acetylmuramoyl-pentapeptide- transferase	-
GA721_RS01260	-	272303..273106 (+)	804	WP_104483911.1	putative RNA methyltransferase	-
GA721_RS01265	ponA	273229..275805 (-)	2577	WP_160242745.1	penicillin-binding protein PBP1a	-
GA721_RS01270	pilM	275977..277035 (+)	1059	WP_075174946.1	pilus assembly protein PilM	Machinery gene
GA721_RS01275	pilN	277035..277670 (+)	636	WP_005180516.1	PilN domain-containing protein	Machinery gene
GA721_RS01280	pilO	277667..278386 (+)	720	WP_005180513.1	type 4a pilus biogenesis protein PilO	Machinery gene
GA721_RS01285	pilP	278386..278913 (+)	528	WP_104489345.1	pilus assembly protein PilP	Machinery gene
GA721_RS01290	pilQ	278974..281112 (+)	2139	WP_168394005.1	type IV pilus secretin PilQ family protein	Machinery gene
GA721_RS01295	aroK	281149..281691 (+)	543	WP_104487954.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38957.59 Da
• Isoelectric Point: 4.5587

>NTDB_id=392157 GA721_RS01270 WP_075174946.1 275977..277035(+) (pilM) [Acinetobacter indicus strain TQ18]
MRRLYRKPSKGLIGVDISSTSVKLLELSVKNDRYWVESYALVPLSEGSVVEKNILNPEAVGDALERAFNLANTPTTAAAL
AIPTSMVISKTIEMDADMTDDEREVQIRMDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRLENVESRCEAIEL
AGLTPKVADVESFAIENAFKVFADTLPMGVNTIGVLDIGHTMTTLSVLKDNKIIYTREQVFGGKQLTQEIQNRYGLSFEE
AGRAKKSRTLPDDYDVEVLEPFLEAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSSKIDIKRIESDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=392157 GA721_RS01270 WP_075174946.1 275977..277035(+) (pilM) [Acinetobacter indicus strain TQ18]
GTGCGCAGGTTATATCGTAAACCGAGTAAGGGGTTAATAGGTGTAGATATTAGTTCTACTTCTGTTAAGTTATTGGAGCT
CTCTGTAAAGAATGATCGTTACTGGGTGGAAAGTTACGCCTTGGTACCGCTCTCAGAGGGCAGTGTGGTTGAGAAAAATA
TTCTGAATCCTGAAGCTGTAGGGGATGCGCTCGAGCGTGCCTTTAATTTGGCCAATACACCGACAACAGCTGCCGCTTTA
GCGATTCCAACCTCAATGGTAATTAGTAAGACCATTGAGATGGATGCCGATATGACGGATGACGAACGTGAAGTTCAGAT
CCGGATGGATGCAGAGCAATATATTCCTTTCCCATTAGATGAAGTTAGCTTGGACTTTGAGGTGCTGCCTGATCGTCTGG
CCAATCCAAACCGGGTTAATGTCTTGCTGGTGGCGACACGACTGGAAAATGTCGAGAGCCGTTGTGAAGCGATTGAACTG
GCCGGTTTAACCCCAAAAGTGGCAGATGTCGAAAGCTTTGCCATTGAAAATGCCTTTAAAGTCTTCGCCGATACCTTGCC
GATGGGGGTAAATACCATTGGTGTGTTAGACATTGGTCATACCATGACCACGCTGTCGGTACTCAAAGACAATAAAATTA
TCTATACCCGCGAGCAGGTCTTTGGTGGCAAGCAGCTGACCCAGGAAATCCAGAACCGTTATGGTCTGTCCTTTGAAGAA
GCCGGCCGCGCCAAGAAAAGCCGTACCTTGCCAGATGATTACGATGTTGAAGTGCTGGAGCCATTCCTTGAGGCGGTGGT
GCAGCAGGCGGCACGTTCGCTACAGTTCTTCTTCTCTTCATCGCAGTTTAATGAAATTGACCATATTCTGCTGGCCGGTG
GGAATGCCAATATTCCGGGCCTGGCCAAGCTGCTCCAGCAAAAACTGGGTTACCGCGTCACCATTGCCAATCCGTTTTTA
CAGATGGGTTTCTCCTCCAAAATTGATATTAAAAGAATTGAAAGCGATGCCTCTTCATTGATGGTGGCATGTGGCTTGGC
ATTGAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: A0A6C0XYY7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	84.091	100	0.841
comM	Acinetobacter nosocomialis M2	84.091	100	0.841
comM	Acinetobacter baylyi ADP1	78.693	100	0.787
pilM	Legionella pneumophila strain ERS1305867	40.96	100	0.412