Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   GBN93_RS16475 Genome accession   NZ_CP045103
Coordinates   3420974..3421711 (-) Length   245 a.a.
NCBI ID   WP_168158667.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain AJ413     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3415974..3426711
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBN93_RS16455 (GBN93_16510) aroB 3416662..3417747 (-) 1086 WP_159152653.1 3-dehydroquinate synthase -
  GBN93_RS16460 (GBN93_16515) aroK 3417778..3418311 (-) 534 WP_062032260.1 shikimate kinase AroK -
  GBN93_RS16465 (GBN93_16520) pilQ 3418349..3420427 (-) 2079 WP_234964931.1 type IV pilus secretin PilQ family protein Machinery gene
  GBN93_RS16470 (GBN93_16525) pilP 3420447..3420974 (-) 528 WP_184117976.1 pilus assembly protein PilP Machinery gene
  GBN93_RS16475 (GBN93_16530) pilO 3420974..3421711 (-) 738 WP_168158667.1 type 4a pilus biogenesis protein PilO Machinery gene
  GBN93_RS16480 (GBN93_16535) pilN 3421708..3422364 (-) 657 WP_184117975.1 PilN domain-containing protein Machinery gene
  GBN93_RS16485 (GBN93_16540) pilM 3422364..3423422 (-) 1059 WP_087527293.1 pilus assembly protein PilM Machinery gene
  GBN93_RS16490 (GBN93_16545) ponA 3423588..3426116 (+) 2529 WP_234964932.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 245 a.a.        Molecular weight: 27720.32 Da        Isoelectric Point: 5.7448

>NTDB_id=391968 GBN93_RS16475 WP_168158667.1 3420974..3421711(-) (pilO) [Acinetobacter johnsonii strain AJ413]
MSRDEFEELTSENHAVTKKKMTVDKFFQQFNTLDPNNYGSWPFSVKLTCWIFIILVIGALGYFVVIKPKLDAISNAHAQE
QNLLNEFREKDSKLRNLQQYQAQLQEMEANFNQQLEQLPKETEIPGLVEDINVSGVNSGLKFKNIRLEPEIRQEFFIEQP
ISIEATGDYHSFGSFVSGIAALPRIVTLHDFDISASSNNDKKTDIPQISYSVKAKTYRYVGATATTDVDVKDTKKDKKAN
AGGAK

Nucleotide


Download         Length: 738 bp        

>NTDB_id=391968 GBN93_RS16475 WP_168158667.1 3420974..3421711(-) (pilO) [Acinetobacter johnsonii strain AJ413]
ATGAGTCGCGATGAATTTGAAGAATTAACATCAGAAAACCATGCTGTAACCAAGAAAAAAATGACGGTTGATAAGTTTTT
TCAACAGTTTAATACCTTAGATCCAAACAATTACGGCAGTTGGCCCTTTTCAGTCAAATTGACTTGTTGGATCTTTATCA
TTTTAGTGATTGGGGCATTGGGTTATTTTGTTGTGATTAAGCCGAAGCTTGATGCGATTTCAAATGCACATGCACAAGAA
CAAAATTTACTGAATGAGTTTCGTGAAAAAGATTCAAAATTACGTAACTTGCAGCAATATCAAGCGCAATTGCAAGAAAT
GGAAGCTAATTTCAATCAGCAGTTAGAACAGTTGCCGAAAGAAACTGAAATTCCTGGTTTGGTTGAAGATATCAATGTGA
GCGGCGTAAATTCTGGATTAAAGTTTAAAAATATTCGTTTAGAGCCAGAAATTCGCCAAGAGTTCTTTATCGAACAGCCG
ATTTCCATTGAAGCGACTGGCGATTATCATTCTTTTGGTTCATTTGTCAGTGGAATTGCAGCTTTACCGCGTATTGTGAC
CTTGCATGACTTCGATATCTCAGCATCAAGTAACAATGATAAAAAGACGGATATTCCGCAAATTTCATACAGTGTAAAAG
CGAAAACCTACCGTTATGTCGGGGCGACGGCTACAACGGATGTTGATGTAAAAGATACAAAAAAAGATAAGAAAGCCAAT
GCGGGAGGTGCCAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(74-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

74.449

92.653

0.69
  comO Acinetobacter baylyi ADP1

72.072

90.612

0.653

Multiple sequence alignment