Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GBN93_RS16465 Genome accession   NZ_CP045103
Coordinates   3418349..3420427 (-) Length   692 a.a.
NCBI ID   WP_234964931.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain AJ413     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3413349..3425427
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBN93_RS16450 (GBN93_16505) - 3415815..3416654 (-) 840 WP_234964930.1 hypothetical protein -
  GBN93_RS16455 (GBN93_16510) aroB 3416662..3417747 (-) 1086 WP_159152653.1 3-dehydroquinate synthase -
  GBN93_RS16460 (GBN93_16515) aroK 3417778..3418311 (-) 534 WP_062032260.1 shikimate kinase AroK -
  GBN93_RS16465 (GBN93_16520) pilQ 3418349..3420427 (-) 2079 WP_234964931.1 type IV pilus secretin PilQ family protein Machinery gene
  GBN93_RS16470 (GBN93_16525) pilP 3420447..3420974 (-) 528 WP_184117976.1 pilus assembly protein PilP Machinery gene
  GBN93_RS16475 (GBN93_16530) pilO 3420974..3421711 (-) 738 WP_168158667.1 type 4a pilus biogenesis protein PilO Machinery gene
  GBN93_RS16480 (GBN93_16535) pilN 3421708..3422364 (-) 657 WP_184117975.1 PilN domain-containing protein Machinery gene
  GBN93_RS16485 (GBN93_16540) pilM 3422364..3423422 (-) 1059 WP_087527293.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 75155.39 Da        Isoelectric Point: 5.2441

>NTDB_id=391966 GBN93_RS16465 WP_234964931.1 3418349..3420427(-) (pilQ) [Acinetobacter johnsonii strain AJ413]
MKNSAKDFIFGDGNTMNHMFRQFSMGAIAMAVMQAASAQVSITNIVPMQIPGQGTEIRVMFNGLPPQPQAYQLEQPSRLV
LDFDKAQQSLKQGSIAVATAEASTVDVTSDAQRSRLTVNLADAGAFTTRVEGNTFILKINSANPALATPVAAARSEQGIS
NIGFQRGSDGEGQVVIDLKGSNTPVDVKQQGSKIVVRVLGNKIPAHLSRRLNVNDFATPVSTIDAQNDGSNGLITIQSNG
SFEYMAYQAEDKLTISLKRPEDKNPMKPKAPSAYTGKKISLDFQDIEVRRVLQLLADFTGINMVASDTVQGNITLRLKDV
PWDQALDIIMKTKNLDKRRNGNVIWIAPVAELIKSEEDEAKAIAQSIKLAPIQTDYIQLSYAKSADVLKLLEDSRDSKGS
VSNRTAGSESLALESLLSARGSAVSDSRTNTLIINDTQQNIDKIRRMIDLLDVAVKQVMVEARIVTASTDFSRELGIKWS
AKKLSGSPVWGGDFQPSFDLGVDLATNTAGTFNFGLLKISDYMLNLELSALQADGHGEVLSAPKVLTGDKQKAYILRGTE
IPYQTWSATEGLKTEFRAANLKLEVTPSITPDGKVQMALMVEKDSIGDITNDGIAIDTSELQTNVLVDDGETVVLGGIFD
DTKRNDYQKVPFLGDLPVVGNLFKNNVKTTIQTELLIFVTPRIVNDSVSRNH

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=391966 GBN93_RS16465 WP_234964931.1 3418349..3420427(-) (pilQ) [Acinetobacter johnsonii strain AJ413]
ATGAAAAATAGTGCTAAAGATTTTATATTTGGGGATGGCAATACGATGAACCATATGTTCCGTCAGTTTTCAATGGGTGC
AATTGCAATGGCGGTGATGCAGGCTGCAAGTGCGCAAGTCAGTATTACGAATATTGTGCCGATGCAAATTCCGGGACAGG
GAACAGAAATTCGTGTCATGTTCAACGGTTTGCCACCACAGCCGCAAGCCTATCAGTTGGAACAGCCATCCCGTTTGGTT
TTGGATTTTGATAAAGCACAGCAGAGCTTAAAACAAGGCAGTATTGCGGTGGCAACGGCTGAAGCAAGTACAGTAGATGT
AACTTCAGATGCGCAGCGGTCACGTTTAACTGTAAATTTGGCGGATGCCGGTGCATTTACTACACGTGTTGAAGGTAATA
CCTTTATTCTGAAAATTAACTCAGCGAACCCGGCGCTAGCAACACCTGTTGCTGCGGCGCGTTCAGAGCAAGGCATTTCA
AATATTGGATTCCAGCGCGGTTCAGATGGTGAAGGCCAAGTTGTCATTGATTTAAAAGGTAGTAATACCCCTGTCGATGT
GAAACAACAAGGCAGTAAAATTGTGGTGCGCGTTTTGGGGAATAAAATTCCTGCCCACTTATCACGCCGTCTCAATGTGA
ATGATTTCGCCACGCCTGTATCGACCATCGATGCACAAAACGATGGCAGTAACGGCCTGATCACAATTCAATCAAATGGC
AGTTTTGAATATATGGCTTATCAGGCAGAAGATAAGCTCACCATTAGTTTAAAGCGTCCTGAAGATAAAAATCCAATGAA
GCCTAAAGCACCAAGTGCTTATACTGGCAAAAAGATTTCTTTAGATTTTCAAGATATTGAAGTGCGACGTGTATTACAGC
TCTTAGCTGATTTTACAGGCATTAACATGGTGGCTTCGGACACAGTACAAGGCAATATTACCTTGCGCCTTAAAGATGTT
CCGTGGGATCAGGCGCTTGATATTATCATGAAAACTAAGAACTTGGATAAGCGACGAAATGGTAATGTGATTTGGATTGC
TCCTGTAGCTGAATTGATCAAATCTGAGGAAGATGAAGCCAAAGCCATCGCACAAAGCATTAAACTTGCTCCGATTCAAA
CTGATTACATTCAATTGAGCTATGCAAAATCAGCGGATGTGTTGAAATTGTTAGAAGACTCTCGTGATAGTAAGGGAAGT
GTATCGAACCGTACAGCGGGTTCAGAGTCATTGGCTTTAGAAAGCTTATTAAGTGCTCGTGGTAGTGCAGTTTCTGATTC
GCGTACCAATACGCTAATTATTAATGATACGCAGCAAAATATTGACAAGATCCGTCGCATGATTGACTTGTTGGATGTCG
CAGTGAAACAGGTGATGGTCGAGGCCCGTATTGTCACAGCATCAACAGACTTTTCTCGCGAGTTGGGGATTAAGTGGAGT
GCGAAGAAGCTTAGCGGTAGCCCAGTTTGGGGCGGGGATTTCCAGCCAAGTTTTGATTTGGGTGTGGATTTAGCAACAAA
CACTGCTGGTACGTTTAACTTTGGTTTATTGAAAATTTCAGACTATATGTTGAATCTTGAGTTAAGCGCGCTTCAAGCGG
ATGGTCATGGTGAAGTGTTATCGGCGCCTAAAGTACTTACTGGCGATAAACAGAAAGCCTATATCTTGCGTGGTACAGAG
ATTCCTTATCAAACATGGAGTGCGACGGAAGGTTTAAAAACTGAGTTTCGCGCGGCTAACTTAAAGTTAGAAGTGACGCC
AAGTATTACTCCAGATGGTAAAGTTCAAATGGCGCTCATGGTTGAAAAAGACTCGATTGGTGATATCACCAATGATGGTA
TTGCGATTGACACCAGTGAATTACAAACCAATGTGTTGGTAGACGATGGTGAAACCGTCGTGCTTGGTGGTATTTTTGAT
GATACGAAACGTAATGACTATCAAAAAGTACCATTCTTAGGAGATTTGCCTGTGGTGGGTAACTTATTTAAGAATAATGT
CAAAACGACGATACAAACTGAGTTGTTGATTTTTGTCACACCACGAATTGTTAATGACAGTGTTTCAAGAAATCATTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(305-348)

AMIN domain (AMIN)

(55-135)

Bacterial type II/III secretion system short domain (Secretin_N)

(376-457)

Bacterial type II and III secretion system protein (Secretin)

(530-684)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Acinetobacter baumannii D1279779

64.681

100

0.675
  pilQ Acinetobacter baumannii strain A118

64.543

100

0.673
  comQ Acinetobacter baylyi ADP1

63.062

100

0.649
  pilQ Pseudomonas aeruginosa PAK

39.017

100

0.39
  pilQ Legionella pneumophila strain Lp02

37.881

99.566

0.377
  pilQ Legionella pneumophila strain ERS1305867

38.166

97.688

0.373
  pilQ Neisseria gonorrhoeae strain FA1090

35.042

100

0.366

Multiple sequence alignment