Detailed information NTG0001217

Experimentally validated

Overview

• Name: comB9
• Type: Machinery gene
• Locus tag: -
• Genome accession: AJ132366
• Coordinates: 1440..2420 (+)
• Length: 326 a.a.
• NCBI ID: CAA10656.1
• Uniprot ID: -
• Organism: Helicobacter pylori P1
• Function: transformation-associated type IV transport system DNA binding and uptake

Function

By complementation of a Hp DeltacomB deletion mutant, we demonstrate that each of the proteins from ComB8 to ComB10 is absolutely essential for the development of natural transformation competence. The putative lipoprotein ComB7 is not essential, but apparently stabilizes the apparatus and modulates the transformation efficiency.

Genomic Context

Location: 1..7420

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Locus_0	comB6	1..571 (+)	571	CAA10653.1	hypothetical protein	Machinery gene
Locus_1	comB7	587..700 (+)	114	CAA10654.1	hypothetical protein	Machinery gene
Locus_2	comB8	697..1440 (+)	744	CAA10655.1	ComB1 protein	Machinery gene
Locus_3	comB9	1440..2420 (+)	981	CAA10656.1	ComB2 protein	Machinery gene
Locus_4	comB10	2413..3543 (+)	1131	CAA10657.1	ComB3 protein	Machinery gene
Locus_5	algA	3613..3962 (+)	350	CAA10658.1	mannose 6 phosphate isomerase	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 37529.28 Da
• Isoelectric Point: 9.6351

>NTDB_id=1217 CAA10656.1 1440..2420(+) (comB9) [Helicobacter pylori P1]
MRKVLYALMGFLLAFSALKADDFLEEANETAPANLNHPMQDLNAIQGSFFDKNRSKMSNTLNIDYFQGQTYKIRLRYAMA
TLLFFSKPISDFVLGDKVGFDAKILESNDRILLIKPLQIGVDSNISVIDSEGKIFSFYVFSTTFTSSKHPNLQVFIEDKN
YYSNAFLKPQNKENVLENALENTPTNNKPLKEKKEETKEKEEETITIGDNTNAMKIVKKDIQKGYKALKSSQRKWYCLGI
CSKKSKLSLMPKEIFNDKQFTYFKFDKRLALSKFPVIYKVVDGYDNPVNTRIVGDYIIAEDVSTKWTLRLGKDYLCIRFV
KKGKDE*

Nucleotide

• Length: 981 bp

>NTDB_id=1217 CAA10656.1 1440..2420(+) (comB9) [Helicobacter pylori P1]
ATGCGTAAGGTTTTATACGCTCTCATGGGCTTTTTGTTGGCTTTTAGTGCTTTAAAAGCCGATGATTTTTTAGAAGAAGC
TAACGAAACAGCCCCAGCGAATTTAAACCACCCCATGCAGGATTTAAACGCCATTCAAGGGAGCTTTTTTGATAAAAACC
GCTCAAAAATGTCCAACACTTTGAACATTGATTACTTTCAAGGGCAAACCTATAAAATCCGCTTGCGTTATGCGATGGCG
ACCTTATTGTTTTTTTCAAAACCCATTAGCGATTTTGTTTTAGGGGATAAGGTGGGCTTTGATGCGAAAATCTTAGAAAG
TAACGATCGTATTTTGCTTATCAAGCCTTTACAAATTGGCGTGGATTCCAATATCAGCGTGATTGATAGCGAAGGTAAGA
TTTTTTCTTTCTATGTGTTTTCTACCACTTTCACCAGCTCCAAACACCCTAATTTGCAGGTTTTCATAGAAGATAAAAAT
TATTATTCTAACGCTTTTTTGAAGCCGCAAAATAAAGAAAATGTCCTTGAAAATGCCCTTGAAAATACCCCCACAAACAA
CAAACCCTTAAAAGAGAAAAAAGAAGAAACCAAAGAAAAAGAAGAAGAGACTATAACTATTGGCGATAACACTAATGCCA
TGAAAATCGTTAAAAAAGACATTCAAAAAGGCTATAAGGCTTTAAAAAGCTCTCAAAGGAAATGGTATTGTTTAGGAATT
TGTTCTAAAAAATCCAAACTCTCTTTGATGCCCAAAGAAATTTTTAACGACAAACAATTCACTTATTTCAAATTTGACAA
AAGATTAGCGCTCTCTAAATTCCCGGTGATTTATAAGGTCGTTGATGGCTATGATAACCCGGTGAATACTAGGATTGTGG
GCGATTACATTATCGCTGAAGACGTTTCTACTAAATGGACTTTAAGGCTGGGTAAGGACTATTTGTGTATCCGTTTTGTC
AAAAAGGGTAAAGATGAATAA

Domains

Predicted by InterproScan.

• Conjugal transfer protein (CagX) (62-317)

References

• [1]: Christopher Corbinais et al. (2017) ComB proteins expression levels determine Helicobacter pylori competence capacity. Scientific Reports 7:41495. [PMID: 28128333]
• [2]: Dirk Hofreuter et al. (2003) Topology and membrane interaction of Helicobacter pylori ComB proteins involved in natural transformation competence. International Journal of Medical Microbiology : IJMM 293(2-3):153-65. [PMID: 12868652]
• [3]: D Hofreuter et al. (2001) Natural transformation competence in Helicobacter pylori is mediated by the basic components of a type IV secretion system. Molecular Microbiology 41(2):379-91. [PMID: 11489125]
• [4]: D Hofreuter et al. (1998) Natural competence for DNA transformation in Helicobacter pylori: identification and genetic characterization of the comB locus. Molecular Microbiology 28(5):1027-38. [PMID: 9663688]