Detailed information NTG0001218

Experimentally validated

Overview

• Name: comB10
• Type: Machinery gene
• Locus tag: -
• Genome accession: AJ132366
• Coordinates: 2413..3543 (+)
• Length: 376 a.a.
• NCBI ID: CAA10657.1
• Uniprot ID: -
• Organism: Helicobacter pylori P1
• Function: transformation-associated type IV transport system DNA binding and uptake

Function

By complementation of a Hp DeltacomB deletion mutant, we demonstrate that each of the proteins from ComB8 to ComB10 is absolutely essential for the development of natural transformation competence. The putative lipoprotein ComB7 is not essential, but apparently stabilizes the apparatus and modulates the transformation efficiency.

Genomic Context

Location: 1..8543

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Locus_0	comB6	1..571 (+)	571	CAA10653.1	hypothetical protein	Machinery gene
Locus_1	comB7	587..700 (+)	114	CAA10654.1	hypothetical protein	Machinery gene
Locus_2	comB8	697..1440 (+)	744	CAA10655.1	ComB1 protein	Machinery gene
Locus_3	comB9	1440..2420 (+)	981	CAA10656.1	ComB2 protein	Machinery gene
Locus_4	comB10	2413..3543 (+)	1131	CAA10657.1	ComB3 protein	Machinery gene
Locus_5	algA	3613..3962 (+)	350	CAA10658.1	mannose 6 phosphate isomerase	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 41944.51 Da
• Isoelectric Point: 9.9021

>NTDB_id=1218 CAA10657.1 2413..3543(+) (comB10) [Helicobacter pylori P1]
MNKWLKGVIVFIGGFATITTISLVYHQKPKAPLNNQPSLLNDDEVKYPLQDYTFTQNPQPTNTESSKDATIKALQEQLKA
ALKALNSKEMNYSKEETFKSPPIDPNTTPPKKDFSLKQLDLLAARITPFKQSPKNYEENLIFPVDNPKGIDGFTNLKEKD
IATNENKLLRTITADKMIPAFLITPISSQIAGKVIAQVESDIFAHMGKAVLIPKGSKVIGYYSNNNKMGEYRLDIVWSRI
ITPHGINIMLTNAKGADIKGYNGLVGELIERNFQRYGVPLLLSTLTNGLLIGITSALNNRGNKEGATNFFGDYLLMQLMR
QSGMGINQVVNQILRDKSKIAPIVVIREGSRVFISPNTDIFFPIPRENEVIAEFLK*

Nucleotide

• Length: 1131 bp

>NTDB_id=1218 CAA10657.1 2413..3543(+) (comB10) [Helicobacter pylori P1]
ATGAATAAGTGGCTTAAGGGGGTGATTGTTTTTATAGGGGGTTTTGCAACGATTACAACCATTTCTTTAGTCTATCATCA
AAAGCCAAAAGCCCCCTTAAATAACCAACCTAGCCTTTTGAATGACGATGAGGTGAAATACCCCTTACAAGACTACACCT
TCACTCAAAACCCACAGCCAACTAACACAGAAAGCTCCAAAGACGCTACCATCAAAGCCTTACAAGAACAGCTCAAAGCC
GCTTTAAAAGCCCTAAACTCCAAAGAAATGAATTATTCCAAAGAAGAAACTTTTAAGAGCCCTCCCATAGATCCAAACAC
AACCCCCCCTAAAAAAGATTTTTCTCTAAAGCAATTAGATTTATTAGCCGCTCGCATCACCCCTTTCAAACAAAGCCCTA
AAAATTACGAAGAAAACCTGATTTTCCCTGTGGATAACCCTAAGGGCATTGATGGTTTCACTAACCTTAAAGAAAAAGAC
ATCGCCACTAATGAAAACAAGCTTTTACGCACCATTACAGCTGACAAAATGATACCCGCTTTTTTGATCACGCCCATTTC
TAGCCAGATCGCTGGTAAAGTGATTGCGCAAGTGGAAAGCGATATTTTCGCTCACATGGGCAAGGCCGTCTTAATCCCCA
AAGGCTCTAAGGTCATAGGCTATTACAGCAACAATAACAAAATGGGCGAATACCGCTTGGATATTGTATGGAGCCGCATC
ATCACTCCCCATGGGATTAATATCATGCTCACTAACGCTAAAGGGGCGGACATTAAAGGCTATAACGGCTTAGTGGGGGA
ATTGATTGAAAGGAATTTCCAGCGCTATGGCGTGCCGTTACTGCTTTCTACTCTCACTAACGGCTTGTTGATTGGAATCA
CTTCGGCTTTAAACAACAGAGGCAATAAAGAAGGAGCCACCAATTTCTTTGGGGATTATCTTTTAATGCAATTGATGAGG
CAAAGCGGCATGGGGATCAATCAAGTGGTCAATCAAATTTTAAGAGACAAGAGCAAGATCGCTCCTATTGTGGTGATTAG
AGAGGGGAGTAGGGTATTCATTTCGCCCAATACTGACATCTTTTTCCCTATACCCAGAGAGAATGAGGTCATCGCTGAGT
TTTTGAAGTGA

Domains

Predicted by InterproScan.

• Bacterial conjugation TrbI-like protein (TrbI) (171-362)

References

• [1]: Christopher Corbinais et al. (2017) ComB proteins expression levels determine Helicobacter pylori competence capacity. Scientific Reports 7:41495. [PMID: 28128333]
• [2]: Dirk Hofreuter et al. (2003) Topology and membrane interaction of Helicobacter pylori ComB proteins involved in natural transformation competence. International Journal of Medical Microbiology : IJMM 293(2-3):153-65. [PMID: 12868652]
• [3]: D Hofreuter et al. (2001) Natural transformation competence in Helicobacter pylori is mediated by the basic components of a type IV secretion system. Molecular Microbiology 41(2):379-91. [PMID: 11489125]
• [4]: D Hofreuter et al. (1998) Natural competence for DNA transformation in Helicobacter pylori: identification and genetic characterization of the comB locus. Molecular Microbiology 28(5):1027-38. [PMID: 9663688]