Detailed information NTG0391953

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: GBN93_RS08750
• Genome accession: NZ_CP045103
• Coordinates: 1845275..1846468 (+)
• Length: 397 a.a.
• NCBI ID: WP_234966381.1
• Uniprot ID: A0AA42U7L6
• Organism: Acinetobacter johnsonii strain AJ413
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1840275..1851468

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GBN93_RS08740 (GBN93_08775)	-	1841385..1843484 (+)	2100	WP_234966377.1	EAL domain-containing protein	-
GBN93_RS08745 (GBN93_08780)	-	1843976..1845037 (+)	1062	WP_234966379.1	3-deoxy-7-phosphoheptulonate synthase	-
GBN93_RS08750 (GBN93_08785)	pilU	1845275..1846468 (+)	1194	WP_234966381.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
GBN93_RS08755 (GBN93_08790)	-	1846516..1847694 (-)	1179	WP_234966383.1	MFS transporter	-
GBN93_RS08760 (GBN93_08795)	gcvH	1847954..1848328 (-)	375	WP_004693240.1	glycine cleavage system protein GcvH	-
GBN93_RS08765 (GBN93_08800)	-	1848401..1849429 (-)	1029	WP_087528086.1	AraC family transcriptional regulator	-

Sequence

Protein

• Length: 397 a.a.
• Molecular weight: 44662.05 Da
• Isoelectric Point: 6.5063

>NTDB_id=391953 GBN93_RS08750 WP_234966381.1 1845275..1846468(+) (pilU) [Acinetobacter johnsonii strain AJ413]
MYSAELLEEARRMMFHMLSKVVEYGGSDLFISADFPPSIKHQGLMKPLGQQNLPSDQTKLFAYSLMNEKQRLEFETELEC
NFAISVPNVSRFRVNVFQQQLHVGMVIRTITAEIPNFTKLQLPTSLKDVIMEKRGLVLVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQALDRIINFFPDERRNQLLMDLSSNMKAIISQRLVRTEDGRGRRAAVEIMLNTPLMSDLILKGNFHELKEVMSKS
RELGMQTFDQALFDLYNQGAIAYEEALRNADSVNELRLQIKLKSSRANPQLSSNSALSFDQAIAEKAKDAEEEKADA

Nucleotide

• Length: 1194 bp

>NTDB_id=391953 GBN93_RS08750 WP_234966381.1 1845275..1846468(+) (pilU) [Acinetobacter johnsonii strain AJ413]
ATGTATTCTGCAGAACTTTTAGAAGAAGCCCGTCGGATGATGTTTCACATGCTCAGCAAAGTGGTTGAGTACGGTGGTTC
AGACTTATTTATATCTGCAGACTTTCCGCCAAGTATTAAGCATCAGGGCCTTATGAAGCCATTGGGTCAACAAAATTTGC
CATCTGATCAGACCAAACTCTTTGCTTATAGTTTAATGAACGAAAAGCAACGATTGGAGTTCGAAACCGAGCTTGAGTGT
AATTTTGCAATTAGCGTTCCCAATGTTTCGCGTTTTCGTGTCAATGTTTTTCAGCAGCAACTGCATGTGGGGATGGTGAT
TCGTACGATTACCGCGGAAATTCCTAACTTCACTAAGTTACAACTTCCAACGTCTTTAAAAGACGTAATTATGGAAAAAC
GTGGTTTGGTCCTCGTTGTCGGTGGTACAGGGTCGGGTAAATCGACGTCTTTAGCAGCCATGATTGATCATCGAAATGAA
AACTCGGCAGGGCACATTATTACGGTTGAAGACCCTGTGGAGTATGTACACAAGCATAAGAAATCGATGATTACTCATCG
GGAAGTGGGTGTAGACTGTCACTCTTGGCACAATGCTTTGAAAAATACTCTGCGCCAAGCGCCCGATGTCATTTTAATTG
GTGAGATTCGTGACACGGAGACGATGGAACATGCCATAGCTTTTGCGGAAACAGGCCACTTATGCCTTGGGACATTACAT
GCTAATAATGCAAATCAGGCTTTAGATCGAATTATTAACTTTTTCCCAGACGAACGTCGCAATCAACTTTTGATGGACTT
ATCTTCCAATATGAAGGCGATTATTTCACAGCGCTTGGTACGAACTGAAGATGGCCGTGGCCGCCGAGCAGCAGTGGAAA
TCATGCTCAATACACCGTTAATGTCAGACTTGATACTTAAAGGGAATTTCCACGAACTGAAAGAAGTGATGTCGAAGTCA
CGAGAGCTCGGTATGCAGACTTTTGATCAGGCTTTGTTTGATTTATACAATCAAGGTGCAATCGCTTATGAAGAGGCGCT
ACGAAATGCAGACTCGGTCAATGAATTACGTCTACAGATTAAGTTAAAGAGTAGTCGTGCCAACCCGCAGTTAAGCTCGA
ATAGTGCTTTGTCTTTTGATCAAGCGATTGCTGAAAAAGCCAAAGACGCTGAAGAAGAGAAGGCCGACGCCTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (122-282)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	59.331	90.428	0.537
pilU	Acinetobacter baylyi ADP1	54.821	91.436	0.501
pilU	Vibrio cholerae strain A1552	50.578	87.154	0.441
pilT	Legionella pneumophila strain Lp02	42.478	85.39	0.363
pilT	Legionella pneumophila strain ERS1305867	42.478	85.39	0.363
pilT	Acinetobacter baylyi ADP1	43.598	82.62	0.36