Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PSM_RS13270 Genome accession   NC_014803
Coordinates   2894729..2896405 (+) Length   558 a.a.
NCBI ID   WP_013465938.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. SM9913     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2889729..2901405
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSM_RS13250 (PSM_A2709) - 2891059..2891481 (+) 423 WP_013465934.1 pilin -
  PSM_RS13255 (PSM_A2710) pilC 2891596..2892825 (+) 1230 WP_013465935.1 type II secretion system F family protein Machinery gene
  PSM_RS13260 (PSM_A2711) pilD 2893050..2893961 (+) 912 WP_041695804.1 A24 family peptidase Machinery gene
  PSM_RS13265 (PSM_A2712) coaE 2893964..2894593 (+) 630 WP_013465937.1 dephospho-CoA kinase -
  PSM_RS13270 (PSM_A2713) pilB 2894729..2896405 (+) 1677 WP_013465938.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSM_RS13275 (PSM_A2714) yacG 2896470..2896700 (+) 231 WP_008114915.1 DNA gyrase inhibitor YacG -
  PSM_RS13280 (PSM_A2715) - 2896979..2897443 (-) 465 WP_008114914.1 Spy/CpxP family protein refolding chaperone -
  PSM_RS13285 (PSM_A2716) - 2897612..2898301 (+) 690 WP_013465939.1 response regulator transcription factor -
  PSM_RS13290 (PSM_A2717) - 2898279..2899706 (+) 1428 WP_013465940.1 ATP-binding protein -
  PSM_RS13295 (PSM_A2718) - 2899696..2900640 (+) 945 WP_013465941.1 alpha/beta fold hydrolase -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62395.71 Da        Isoelectric Point: 6.2136

>NTDB_id=39161 PSM_RS13270 WP_013465938.1 2894729..2896405(+) (pilB) [Pseudoalteromonas sp. SM9913]
MNINSPLLRKFITLGRVDTDTVKTRQQDFASTAELISKCGKIDSKDLAEQCIDLFRVPFFDLKDFNVAQISAELVKEKLI
RKHHILPLVQKGRKVYIAASDPTDYGAFENFEFSTGLSCEVVVVDYVQLDHKIEQLLDASGSLNLSEDEFKEFAELDSEQ
HKEHTPKDDDKDDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRIRFRIDGILHEVASPPNTLASKLSARIKVMSRLDI
AEKRKPQDGRIKLKVTERKSIDFRVSTMPTLWGEKIVMRILDSSSAMLGIDVLGYEPEQKKLYLDALEQPQGMILVTGPT
GSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGVNQVQINIKADMTFANALRAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETITRLLNMGVPAYNVASSISLIIAQRLARRLCSKCKTPEQLPREELIRQGFSEQQIQDMVL
YAPKGCENCTDGYKGRVGIYEVMKITPEIAQIIMRGGNSLEIAEVSLKAGYNNLRLSGIRKAAEGVTSLAEVNRVTSF

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=39161 PSM_RS13270 WP_013465938.1 2894729..2896405(+) (pilB) [Pseudoalteromonas sp. SM9913]
ATGAATATTAACTCACCGTTATTAAGAAAATTTATTACCCTCGGCCGGGTTGATACTGATACCGTAAAAACACGGCAGCA
AGATTTTGCGTCAACTGCAGAGCTTATCTCTAAATGTGGAAAAATAGACAGTAAAGATTTAGCTGAGCAATGCATTGATT
TATTTAGAGTGCCTTTTTTTGATTTAAAAGATTTTAATGTTGCACAAATATCAGCTGAGCTGGTTAAAGAAAAACTGATC
CGCAAACACCATATTTTGCCTTTGGTACAAAAAGGCAGAAAAGTGTATATTGCCGCCTCAGATCCTACCGATTATGGTGC
CTTCGAAAACTTTGAATTTAGCACGGGTTTATCGTGCGAAGTAGTTGTGGTTGACTACGTCCAGCTTGACCATAAAATTG
AGCAATTACTCGATGCCTCTGGCAGTTTAAATTTAAGTGAAGACGAATTTAAAGAGTTTGCTGAACTCGATTCTGAGCAG
CACAAAGAACACACTCCTAAAGACGATGACAAAGACGACGCCCCAATAATTGTCTACATTAATAAAATTTTAATGGATGC
AATAAAAAAAGGCGCCTCAGATTTACACTTTGAACCTTATGAACACAAATACCGTATTCGCTTTCGTATTGATGGTATTT
TGCACGAAGTAGCCAGCCCGCCAAATACATTGGCCAGTAAGCTATCGGCGCGAATAAAAGTAATGTCGCGACTGGATATT
GCAGAAAAGCGTAAACCACAAGATGGCCGTATTAAGCTTAAGGTAACAGAGCGTAAAAGTATTGATTTTCGTGTTAGTAC
CATGCCAACTTTGTGGGGCGAAAAAATTGTAATGCGTATTCTTGATTCATCCAGTGCCATGTTAGGTATAGATGTATTAG
GCTATGAACCCGAGCAGAAAAAACTATATCTCGATGCACTTGAGCAACCGCAAGGAATGATTTTGGTTACCGGCCCAACC
GGTTCAGGTAAAACGGTTTCTTTGTATACGGGCTTAAATATACTCAATCAGCCGGAGCGGAATATTAGTACCGCCGAAGA
TCCGGTAGAAATTAACCTCGAAGGGGTTAACCAAGTACAAATAAATATTAAAGCCGATATGACCTTTGCCAATGCATTAC
GGGCGTTTTTACGTCAAGATCCCGATGTGGTGATGGTGGGTGAAATACGTGATTTAGAAACTGCAGAAATATCAATTAAA
GCGGCGCAAACAGGTCACTTGGTACTGTCAACACTGCATACCAACTCAGCGCCAGAAACGATTACTCGTTTGTTAAATAT
GGGTGTACCCGCGTATAATGTGGCCAGTTCTATTAGTTTAATTATAGCTCAGCGTTTAGCCCGAAGATTATGCTCTAAAT
GTAAAACACCAGAGCAGTTACCTCGCGAAGAGCTTATACGCCAAGGCTTTAGTGAACAACAAATTCAAGACATGGTTTTA
TACGCACCTAAAGGCTGTGAAAACTGTACCGATGGCTACAAAGGCCGTGTTGGTATTTACGAAGTAATGAAAATTACGCC
TGAAATAGCGCAAATTATAATGCGCGGCGGTAACTCATTAGAAATTGCTGAGGTATCACTTAAAGCAGGATATAACAATT
TGCGTTTATCGGGTATTCGTAAAGCCGCAGAGGGTGTGACCTCACTTGCTGAGGTTAATCGTGTCACTAGTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)

Type II/IV secretion system protein (T2SSE)

(182-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.778

96.774

0.559
  pilB Acinetobacter baylyi ADP1

58.218

90.502

0.527
  pilB Legionella pneumophila strain ERS1305867

55.273

91.756

0.507
  pilB Vibrio cholerae strain A1552

52.247

95.699

0.5
  pilB Vibrio parahaemolyticus RIMD 2210633

51.873

95.699

0.496
  pilB Vibrio campbellii strain DS40M4

51.311

95.699

0.491
  pilF Neisseria gonorrhoeae MS11

50

93.548

0.468
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.575

92.832

0.367

Multiple sequence alignment