Detailed information NTG0391531

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: GBN67_RS07920
• Genome accession: NZ_CP045051
• Coordinates: 1644161..1645354 (-)
• Length: 397 a.a.
• NCBI ID: WP_234964034.1
• Uniprot ID: -
• Organism: Acinetobacter johnsonii strain AJ312
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1639161..1650354

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GBN67_RS07905 (GBN67_07940)	-	1641202..1642230 (+)	1029	WP_004979806.1	AraC family transcriptional regulator	-
GBN67_RS07910 (GBN67_07945)	gcvH	1642302..1642676 (+)	375	WP_004693240.1	glycine cleavage system protein GcvH	-
GBN67_RS07915 (GBN67_07950)	-	1642935..1644113 (+)	1179	WP_234964033.1	MFS transporter	-
GBN67_RS07920 (GBN67_07955)	pilU	1644161..1645354 (-)	1194	WP_234964034.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
GBN67_RS07925 (GBN67_07960)	-	1645594..1646655 (-)	1062	WP_234964035.1	3-deoxy-7-phosphoheptulonate synthase	-
GBN67_RS16250	-	1646634..1646747 (+)	114	WP_157843581.1	hypothetical protein	-
GBN67_RS07930 (GBN67_07965)	-	1647140..1649239 (-)	2100	WP_234964036.1	EAL domain-containing protein	-

Sequence

Protein

• Length: 397 a.a.
• Molecular weight: 44647.04 Da
• Isoelectric Point: 6.6215

>NTDB_id=391531 GBN67_RS07920 WP_234964034.1 1644161..1645354(-) (pilU) [Acinetobacter johnsonii strain AJ312]
MYSAELLEEARRMMFHMLSKVVEYGGSDLFISADFPPSIKHQGLMKPLGQQNLPSDQTKLFAYSLMNEKQRLEFETELEC
NFAISVPNVSRFRVNVFQQQLHVGMVIRTITAEIPNFTKLQLPTSLKDVIMEKRGLVLVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQALDRIINFFPDERRNQLLMDLSSNMKAIISQRLVRTEDGRGRRAAVEIMLNTPLMSDLILKGNFHELKEVMSKS
RELGMQTFDQALFDLYNQGAIAYEEALRNADSVNELRLQIKLKSSRANPQLSSNSALSFDQAVAEKAKDAEEEKANA

Nucleotide

• Length: 1194 bp

>NTDB_id=391531 GBN67_RS07920 WP_234964034.1 1644161..1645354(-) (pilU) [Acinetobacter johnsonii strain AJ312]
ATGTATTCTGCAGAACTTTTAGAAGAAGCCCGTCGAATGATGTTTCACATGCTCAGCAAAGTGGTTGAGTATGGTGGTTC
AGACTTATTTATATCTGCAGACTTTCCGCCAAGTATTAAGCATCAGGGCCTCATGAAGCCACTGGGTCAACAAAACTTGC
CATCTGATCAGACCAAACTTTTTGCTTATAGTTTAATGAACGAAAAGCAACGATTGGAGTTCGAAACTGAGCTGGAGTGT
AATTTTGCAATTAGCGTTCCCAATGTTTCACGTTTTCGCGTCAATGTTTTTCAGCAGCAACTGCATGTAGGTATGGTGAT
TCGTACGATTACTGCGGAAATTCCTAACTTCACTAAGTTACAACTTCCAACGTCTTTAAAAGACGTAATTATGGAAAAAC
GTGGTTTGGTCCTCGTTGTCGGTGGTACAGGGTCGGGTAAATCGACGTCTTTGGCAGCCATGATTGATCATCGAAATGAA
AACTCGGCAGGGCACATTATTACGGTTGAAGATCCTGTGGAGTATGTACACAAGCATAAGAAATCGATGATTACTCATCG
GGAAGTGGGTGTAGACTGTCATTCTTGGCACAATGCTTTGAAAAATACTCTGCGCCAAGCGCCCGATGTCATTTTAATTG
GTGAGATTCGTGACACGGAGACGATGGAACATGCCATAGCTTTTGCAGAAACAGGTCACTTATGCCTAGGAACACTGCAT
GCTAATAATGCAAATCAGGCTTTAGATCGAATTATTAACTTTTTCCCAGACGAACGTCGCAATCAACTTTTGATGGACTT
ATCTTCCAATATGAAGGCGATTATTTCACAGCGTCTGGTGCGAACTGAAGATGGGCGTGGCCGCCGAGCAGCAGTGGAAA
TCATGCTCAATACACCGTTGATGTCAGACTTGATACTTAAAGGGAATTTCCACGAACTCAAAGAAGTGATGTCGAAATCA
CGAGAGTTAGGCATGCAGACTTTTGATCAGGCTTTGTTTGATTTATACAATCAAGGTGCAATCGCTTATGAAGAAGCACT
ACGAAATGCAGACTCGGTCAATGAATTACGTCTACAGATTAAATTAAAGAGTAGTCGTGCCAACCCGCAGTTAAGCTCAA
ATAGTGCTTTGTCTTTTGATCAAGCGGTTGCTGAAAAAGCCAAAGACGCTGAAGAAGAGAAGGCCAACGCCTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (122-282)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	59.331	90.428	0.537
pilU	Acinetobacter baylyi ADP1	54.821	91.436	0.501
pilU	Vibrio cholerae strain A1552	50.578	87.154	0.441
pilT	Legionella pneumophila strain Lp02	42.478	85.39	0.363
pilT	Legionella pneumophila strain ERS1305867	42.478	85.39	0.363
pilT	Acinetobacter baylyi ADP1	43.598	82.62	0.36