Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   F9Z45_RS16610 Genome accession   NZ_CP044975
Coordinates   3574533..3576266 (+) Length   577 a.a.
NCBI ID   WP_159607832.1    Uniprot ID   A0A6P1IHK4
Organism   Hydrogenophaga sp. PBL-H3 substr. PBL-H3(B2)     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3569533..3581266
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F9Z45_RS16575 (F9Z45_16570) - 3569576..3570028 (+) 453 WP_159607831.1 CNP1-like family protein -
  F9Z45_RS16580 (F9Z45_16575) proB 3570048..3571211 (-) 1164 WP_159608776.1 glutamate 5-kinase -
  F9Z45_RS16585 (F9Z45_16580) obgE 3571241..3572311 (-) 1071 WP_159608777.1 GTPase ObgE -
  F9Z45_RS16590 (F9Z45_16585) rpmA 3572450..3572707 (-) 258 WP_056273119.1 50S ribosomal protein L27 -
  F9Z45_RS16595 (F9Z45_16590) rplU 3572721..3573032 (-) 312 WP_159608778.1 50S ribosomal protein L21 -
  F9Z45_RS16600 (F9Z45_16595) - 3573305..3574234 (+) 930 WP_159608779.1 polyprenyl synthetase family protein -
  F9Z45_RS16610 (F9Z45_16605) pilB 3574533..3576266 (+) 1734 WP_159607832.1 type IV-A pilus assembly ATPase PilB Machinery gene
  F9Z45_RS16615 (F9Z45_16610) pilC 3576299..3577516 (+) 1218 WP_159607833.1 type II secretion system F family protein Machinery gene
  F9Z45_RS16620 (F9Z45_16615) - 3577516..3578376 (+) 861 WP_159607834.1 A24 family peptidase -
  F9Z45_RS16625 (F9Z45_16620) coaE 3578373..3578990 (+) 618 WP_159607835.1 dephospho-CoA kinase -
  F9Z45_RS16630 (F9Z45_16625) zapD 3579033..3579788 (+) 756 WP_159607836.1 cell division protein ZapD -
  F9Z45_RS16635 (F9Z45_16630) - 3579808..3580011 (+) 204 WP_159607837.1 DNA gyrase inhibitor YacG -
  F9Z45_RS16640 (F9Z45_16635) - 3580027..3580488 (-) 462 WP_159607838.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62671.19 Da        Isoelectric Point: 5.6301

>NTDB_id=391074 F9Z45_RS16610 WP_159607832.1 3574533..3576266(+) (pilB) [Hydrogenophaga sp. PBL-H3 substr. PBL-H3(B2)]
MASVESVTQETPSMALPGLGRALVSAGKLGQKAAEDLYRKAQSGRSSFIAELTGSGAVSASDLAHTMSVAFSAPLLDLDA
IDVQRLPSGLLDAKICADYRIVVLSKRNNRLMVATADPADQQAAEKIKFATQMGVDWVIAEYDKLSKLVESLTTSVSETM
DNIIGGEFEFDEMATEAVVADTNDKTSEVDDAPVVKFLHKMLIDAFNMRASDLHFEPYEHNYRVRFRVDGELREIASPPI
AIKDKLASRIKVISRMDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKVGIDALGYEPEEKER
LMAAIVRPYGMVLVTGPTGSGKTVSLYTCLNILNKPGINISTAEDPSEINLPGVNQVNVNEKAGLTFAAALKAFLRQDPD
VIMVGEIRDLETADISIKAAQTGHMVLSTLHTNDAPTTLTRMMNMGIPTFNIASSVILITAQRLARRLCGVCKAPLDVPR
KALVDAGYKAEDLDGTWTPYRPVGCSACNNGYKGRVGIYQVMPITEEIQRIILRGGSALDIAQQASKEGVRTLRESGLLK
VKLGMTSLEEVLSVTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=391074 F9Z45_RS16610 WP_159607832.1 3574533..3576266(+) (pilB) [Hydrogenophaga sp. PBL-H3 substr. PBL-H3(B2)]
ATGGCGTCCGTTGAATCTGTCACTCAGGAAACCCCGTCCATGGCGCTGCCTGGCTTGGGTCGTGCGTTGGTGTCAGCAGG
GAAACTCGGGCAAAAAGCAGCCGAGGATCTTTACCGCAAAGCTCAGAGTGGCAGATCCAGCTTTATCGCTGAACTCACGG
GCTCAGGTGCTGTGTCGGCGTCGGATCTGGCACACACCATGTCGGTGGCGTTCTCCGCTCCACTCCTTGATCTCGACGCG
ATCGATGTGCAGCGCCTGCCCAGTGGCTTGCTGGATGCAAAGATCTGCGCTGACTACCGCATTGTGGTGCTCAGCAAACG
CAACAACCGTCTGATGGTGGCGACGGCTGATCCAGCCGATCAGCAAGCGGCCGAAAAGATTAAATTCGCCACGCAGATGG
GCGTTGACTGGGTGATTGCTGAGTACGACAAGCTCAGCAAGCTGGTGGAGTCACTGACCACCAGCGTCTCCGAAACGATG
GACAACATCATTGGAGGGGAGTTCGAGTTCGATGAAATGGCGACCGAGGCGGTGGTCGCGGACACCAACGACAAGACTTC
TGAAGTCGACGACGCTCCGGTGGTCAAGTTTCTGCACAAGATGTTGATCGATGCATTCAACATGCGTGCTTCTGACTTGC
ACTTCGAACCCTACGAGCACAACTACCGCGTTCGATTCCGGGTCGATGGCGAACTGCGCGAGATTGCCTCACCTCCCATC
GCCATCAAGGACAAACTGGCTTCCCGCATCAAGGTGATTTCCCGTATGGACATCTCTGAGAAGCGGGTACCCCAAGACGG
CCGAATGAAGCTCAAGATCGGGCCGGATCGTGTGATCGATTTCCGGGTGAGCACACTGCCGACCCTTTTCGGTGAAAAGA
TCGTGATCCGTATTCTGGACCCCAGCAGCGCCAAGGTGGGCATTGATGCGCTGGGTTACGAGCCTGAAGAAAAAGAGCGG
CTGATGGCGGCCATCGTGCGACCCTATGGCATGGTGCTGGTGACCGGCCCTACCGGTTCGGGCAAGACAGTCTCGCTCTA
CACCTGCCTGAACATCCTCAACAAGCCAGGCATCAACATCTCAACCGCAGAGGATCCGTCCGAGATCAACTTGCCAGGGG
TGAACCAAGTCAACGTGAATGAGAAGGCGGGCTTGACGTTTGCAGCGGCATTGAAGGCTTTCCTGCGGCAGGATCCTGAT
GTCATCATGGTGGGCGAGATTCGGGATCTGGAAACTGCCGACATTTCCATCAAGGCCGCGCAGACCGGACACATGGTGCT
GTCCACGCTCCACACCAATGACGCGCCGACTACCCTGACGCGGATGATGAACATGGGCATTCCCACGTTCAACATTGCAT
CCAGCGTGATCCTGATCACCGCTCAGCGTTTGGCCAGGCGGCTGTGCGGCGTGTGCAAAGCCCCTTTGGACGTGCCACGC
AAGGCGTTGGTCGATGCGGGCTACAAGGCAGAGGATCTGGACGGCACCTGGACGCCCTACCGACCTGTGGGCTGCTCGGC
CTGCAACAACGGCTACAAGGGCAGGGTTGGCATCTACCAGGTAATGCCGATCACCGAAGAAATTCAACGCATCATCCTGC
GCGGCGGAAGCGCGCTGGACATTGCACAACAAGCCAGCAAGGAGGGCGTCCGCACGCTGCGTGAGTCCGGCCTGCTCAAG
GTGAAGTTGGGCATGACTTCACTGGAAGAAGTGCTCAGCGTGACCAACGAGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-152)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6P1IHK4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.367

97.227

0.529
  pilB Acinetobacter baumannii D1279779

54.189

97.227

0.527
  pilF Neisseria gonorrhoeae MS11

52.753

97.574

0.515
  pilB Legionella pneumophila strain ERS1305867

50.088

98.96

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

46.071

97.054

0.447
  pilB Vibrio campbellii strain DS40M4

44.583

97.574

0.435
  pilB Vibrio cholerae strain A1552

47.876

89.775

0.43
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.434

98.614

0.369
  pilF Thermus thermophilus HB27

37.148

98.44

0.366

Multiple sequence alignment