Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   F9K07_RS25205 Genome accession   NZ_CP044549
Coordinates   5453942..5455675 (+) Length   577 a.a.
NCBI ID   WP_159596020.1    Uniprot ID   A0A6P1J816
Organism   Hydrogenophaga sp. BPS33     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5448942..5460675
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F9K07_RS25170 (F9K07_25125) - 5448943..5449476 (+) 534 WP_159596018.1 CNP1-like family protein -
  F9K07_RS25175 (F9K07_25130) proB 5449495..5450634 (-) 1140 WP_159597098.1 glutamate 5-kinase -
  F9K07_RS25180 (F9K07_25135) obgE 5450684..5451757 (-) 1074 WP_159597099.1 GTPase ObgE -
  F9K07_RS25185 (F9K07_25140) rpmA 5451897..5452154 (-) 258 WP_159596019.1 50S ribosomal protein L27 -
  F9K07_RS25190 (F9K07_25145) rplU 5452168..5452479 (-) 312 WP_159597100.1 50S ribosomal protein L21 -
  F9K07_RS25195 (F9K07_25150) - 5452673..5453656 (+) 984 WP_236581509.1 polyprenyl synthetase family protein -
  F9K07_RS25205 (F9K07_25160) pilB 5453942..5455675 (+) 1734 WP_159596020.1 type IV-A pilus assembly ATPase PilB Machinery gene
  F9K07_RS25210 (F9K07_25165) pilC 5455710..5456927 (+) 1218 WP_159596021.1 type II secretion system F family protein Machinery gene
  F9K07_RS25215 (F9K07_25170) - 5456927..5457781 (+) 855 WP_159596022.1 A24 family peptidase -
  F9K07_RS25220 (F9K07_25175) coaE 5457778..5458392 (+) 615 WP_159596023.1 dephospho-CoA kinase -
  F9K07_RS25225 (F9K07_25180) zapD 5458436..5459191 (+) 756 WP_159597102.1 cell division protein ZapD -
  F9K07_RS25230 (F9K07_25185) - 5459199..5459402 (+) 204 WP_159596024.1 DNA gyrase inhibitor YacG -
  F9K07_RS25235 (F9K07_25190) - 5459442..5459885 (-) 444 WP_328794067.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62584.00 Da        Isoelectric Point: 5.6243

>NTDB_id=390958 F9K07_RS25205 WP_159596020.1 5453942..5455675(+) (pilB) [Hydrogenophaga sp. BPS33]
MASIETASPDTPSMALPGLGRALVSAGKLAQKAAEDLYRKAQSGRTSFIAELTGSGAVSPSDLAHTMSVAFAAPLLDLDA
IDVQRLPSGLLDAKICADYRIVVLSKRNNRLMVATADPADQQAAEKIKFATQMGVDWVIAEYDKLSKLVETQTTNVNDTM
DNIIGGDFEFDEVAAETVVNDATDKASEVDDAPVVKFLHKMLIDAFNMRASDLHFEPYEHNYRVRFRVDGELREIASPPI
AIKDKLASRIKVISRMDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKVGIDALGYEPEEKER
LLNAIGRPYGMVLVTGPTGSGKTVSLYTCLNILNKPGINISTAEDPSEINLPGVNQVNVNEKAGLTFAAALKAFLRQDPD
VIMVGEIRDLETADISIKAAQTGHMVLSTLHTNDAPTTLTRMMNMGIPTFNIASSVILITAQRLARRLCATCKAPLDVPR
KALVDAGFKAEDLDGTWTPYKPVGCSACNNGYKGRVGIYQVMPISEEIQRIILRGGSALDIAQQASKEGVRTLRESGLLK
VKLGMTSLEEVLSVTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=390958 F9K07_RS25205 WP_159596020.1 5453942..5455675(+) (pilB) [Hydrogenophaga sp. BPS33]
ATGGCGTCTATCGAAACAGCCTCCCCTGACACCCCATCCATGGCCCTGCCCGGACTCGGACGCGCACTTGTGTCCGCCGG
AAAGCTGGCTCAGAAGGCCGCAGAAGACCTCTACCGCAAGGCTCAAAGCGGGCGCACCAGCTTCATCGCCGAACTGACCG
GCTCGGGAGCGGTCTCTCCTTCGGATCTCGCCCATACCATGTCCGTCGCATTCGCGGCCCCCTTGCTGGATCTGGATGCC
ATCGACGTTCAGCGCCTGCCCAGTGGACTGCTGGACGCAAAGATTTGTGCCGACTACCGCATCGTGGTGCTGAGCAAGCG
CAACAACCGCTTGATGGTCGCTACGGCGGATCCTGCCGATCAACAGGCGGCCGAGAAAATCAAGTTCGCGACGCAAATGG
GCGTCGACTGGGTCATTGCCGAATACGACAAGCTCAGCAAACTGGTGGAAACACAAACCACCAATGTCAACGACACGATG
GACAACATCATCGGTGGCGACTTCGAGTTCGACGAAGTGGCCGCCGAAACAGTCGTCAACGACGCGACCGACAAGGCTTC
GGAAGTCGACGATGCACCTGTGGTGAAGTTCCTTCACAAGATGCTGATTGACGCCTTCAACATGCGCGCGTCCGACTTGC
ACTTCGAGCCGTACGAGCACAACTACCGAGTCCGTTTTCGCGTGGACGGCGAGTTGCGCGAAATTGCCTCGCCGCCGATT
GCCATCAAGGACAAGCTGGCTTCGCGCATCAAGGTGATCTCGCGCATGGACATCTCCGAAAAGCGAGTACCTCAAGACGG
TCGAATGAAGCTCAAAATCGGTCCCGATCGGGTCATCGATTTCCGCGTCAGCACGTTGCCCACGCTCTTTGGCGAAAAGA
TCGTGATCCGCATTCTGGATCCCAGCAGTGCGAAAGTGGGCATTGATGCCCTGGGCTACGAGCCCGAGGAAAAGGAGCGG
CTGCTCAACGCCATTGGTCGGCCCTACGGCATGGTGCTGGTCACCGGCCCGACAGGCTCTGGCAAGACCGTGTCGCTCTA
CACCTGCTTGAACATCCTGAACAAGCCGGGCATCAACATCTCGACCGCCGAAGATCCCTCCGAAATCAATTTGCCCGGCG
TCAACCAGGTCAACGTCAATGAGAAGGCCGGACTCACGTTCGCCGCGGCGCTGAAGGCGTTTCTGCGTCAGGACCCGGAT
GTGATCATGGTGGGGGAGATTCGCGATCTGGAAACTGCCGACATCTCGATCAAGGCGGCTCAAACCGGCCACATGGTGCT
GTCGACCCTTCACACGAACGATGCACCTACCACGCTGACACGGATGATGAACATGGGCATCCCCACGTTCAATATTGCAT
CCAGCGTGATTCTGATCACCGCTCAACGACTGGCACGGCGCCTTTGCGCCACCTGCAAGGCGCCACTCGATGTGCCACGA
AAGGCGCTCGTCGATGCGGGATTCAAGGCAGAGGATCTGGATGGCACCTGGACGCCCTACAAGCCGGTCGGCTGCTCTGC
GTGCAACAACGGCTACAAAGGCCGCGTCGGCATCTACCAGGTCATGCCCATTTCAGAAGAAATCCAGCGCATCATTCTGC
GTGGAGGCAGCGCATTGGATATCGCGCAGCAAGCCAGCAAGGAAGGGGTTCGCACGCTTCGCGAATCGGGCTTGCTCAAG
GTCAAGCTGGGAATGACTTCGCTCGAAGAGGTTCTGAGCGTGACCAACGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(53-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6P1J816

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.24

98.094

0.532
  pilB Acinetobacter baylyi ADP1

54.011

97.227

0.525
  pilF Neisseria gonorrhoeae MS11

53.108

97.574

0.518
  pilB Legionella pneumophila strain ERS1305867

49.737

98.96

0.492
  pilB Vibrio parahaemolyticus RIMD 2210633

45.098

97.227

0.438
  pilB Vibrio campbellii strain DS40M4

43.739

98.267

0.43
  pilB Vibrio cholerae strain A1552

48.062

89.428

0.43
  pilF Thermus thermophilus HB27

37.676

98.44

0.371
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.434

98.614

0.369

Multiple sequence alignment