Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HLG75_RS15740 Genome accession   NZ_CP053215
Coordinates   3239292..3240782 (-) Length   496 a.a.
NCBI ID   WP_001166321.1    Uniprot ID   V5VGM1
Organism   Acinetobacter baumannii strain DT0544C     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3234292..3245782
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HLG75_RS15710 (HLG75_15730) gigA 3235286..3236254 (+) 969 WP_000273589.1 RsbU family protein phosphatase GigA -
  HLG75_RS15715 (HLG75_15735) gigB 3236357..3236878 (+) 522 WP_000103065.1 anti-anti-sigma factor GigB -
  HLG75_RS15720 (HLG75_15740) - 3236905..3237729 (+) 825 WP_000177096.1 carbon-nitrogen hydrolase family protein -
  HLG75_RS15725 (HLG75_15745) - 3237744..3238121 (+) 378 WP_000266123.1 DUF4951 domain-containing protein -
  HLG75_RS15730 (HLG75_15750) - 3238281..3238718 (+) 438 WP_000371731.1 MarR family winged helix-turn-helix transcriptional regulator -
  HLG75_RS15735 (HLG75_15755) - 3238806..3239237 (+) 432 WP_109426665.1 organic hydroperoxide resistance protein -
  HLG75_RS15740 (HLG75_15760) pilB 3239292..3240782 (-) 1491 WP_001166321.1 type II secretion system ATPase GspE Machinery gene
  HLG75_RS15745 (HLG75_15765) - 3240879..3241700 (+) 822 WP_000462729.1 hypothetical protein -
  HLG75_RS15750 (HLG75_15770) - 3241710..3242627 (+) 918 WP_000785635.1 mechanosensitive ion channel family protein -
  HLG75_RS15755 (HLG75_15775) - 3242617..3245424 (-) 2808 WP_000276872.1 DUF2339 domain-containing protein -

Sequence

Protein


Download         Length: 496 a.a.        Molecular weight: 55329.22 Da        Isoelectric Point: 6.2037

>NTDB_id=390617 HLG75_RS15740 WP_001166321.1 3239292..3240782(-) (pilB) [Acinetobacter baumannii strain DT0544C]
MQILKQIQVPYSFAKRHGVLFRYDGDQVFIVRRQNTEKIALQEARRILGKPAHYQLCTEQEFNSLLSTSYAGDTGESQQV
AAGLEDHPDLLSLADQVPETEDLMDQEDDAPIVRLINALLSEAIRVGASDIHIEAFEKKLSVRLRVDGQLREIVQPRREL
APLLVSRIKVMAKLDIAEKRVPQDGRISLRLAGREVDVRVSTLPSSHGERVVMRLLDKQAGRLNMTHLGLMANDYERLTQ
LVHRPHGIILVTGPTGSGKTTTLYAALSDLNDNTRNILTAEDPIEYQLEGIGQTQVNTKVDMTFARALKAMLRQDPDVVM
VGEIRDLETAEIAVQASLTGHLVLSTLHTNTAIGAVTRLKDMGIEPFLLSSSLIGVVAQRLVRTLCPHCMTWREADTFEK
QVFQHISHEPSLKLPEAQGCDQCSHLGFNGRTAIYEIVPIDEPMRRLIHGNAAEFELENHARQYSGSIRDDGLRKVLSGK
TTLEEVLRVTNEASEA

Nucleotide


Download         Length: 1491 bp        

>NTDB_id=390617 HLG75_RS15740 WP_001166321.1 3239292..3240782(-) (pilB) [Acinetobacter baumannii strain DT0544C]
ATGCAAATATTGAAACAAATACAAGTTCCATATAGTTTTGCCAAGCGTCATGGTGTTTTATTTCGTTATGACGGTGATCA
AGTTTTTATTGTTAGACGTCAAAATACAGAAAAAATTGCATTGCAGGAAGCAAGACGAATTTTGGGGAAACCTGCACACT
ATCAATTATGCACTGAACAAGAATTTAATAGTCTATTAAGTACCAGTTATGCAGGTGATACAGGTGAATCTCAACAAGTA
GCTGCAGGCTTAGAAGACCATCCGGATTTATTAAGCCTTGCAGATCAGGTGCCTGAAACAGAAGACTTAATGGATCAAGA
GGATGACGCGCCTATTGTTCGTCTAATCAATGCACTTCTCTCTGAGGCAATTCGTGTTGGAGCTTCAGATATCCATATTG
AAGCCTTTGAGAAAAAGCTATCAGTTCGTTTACGGGTAGATGGTCAATTACGTGAAATTGTGCAGCCACGCCGTGAACTA
GCCCCGTTACTTGTTTCGCGTATTAAAGTTATGGCGAAACTCGATATTGCTGAAAAGCGTGTACCGCAAGATGGACGTAT
TTCTTTACGTCTTGCTGGTCGTGAAGTCGATGTCCGTGTTTCAACTTTGCCATCGTCACATGGTGAAAGGGTGGTCATGC
GTTTACTCGATAAACAAGCGGGTCGCCTTAATATGACTCACCTAGGTTTAATGGCAAATGACTATGAACGTTTAACTCAG
TTAGTACATCGTCCGCACGGTATTATTTTGGTCACAGGGCCTACTGGTTCGGGGAAAACGACGACTTTATATGCTGCCTT
ATCTGATCTAAATGACAATACCCGTAACATCTTAACCGCCGAAGATCCAATTGAGTATCAGCTTGAAGGTATTGGCCAAA
CTCAAGTGAATACCAAAGTGGATATGACATTTGCTCGTGCTTTAAAGGCAATGTTACGTCAAGATCCAGATGTGGTCATG
GTGGGGGAGATTCGTGATCTGGAAACTGCTGAAATTGCGGTGCAGGCATCCTTAACAGGTCATTTGGTTTTATCGACGCT
ACATACCAACACCGCTATTGGTGCAGTGACTCGACTTAAAGATATGGGGATTGAACCTTTCCTGCTTTCAAGTTCTTTAA
TTGGGGTGGTTGCGCAGCGTTTGGTTCGTACTTTATGTCCACATTGTATGACATGGCGTGAAGCCGATACTTTTGAGAAG
CAAGTTTTTCAGCATATTTCTCATGAACCAAGTTTAAAGTTACCAGAGGCTCAAGGGTGTGATCAGTGTTCTCATTTAGG
GTTTAATGGCCGTACAGCGATTTATGAAATTGTACCTATTGATGAACCTATGCGCCGTCTCATTCATGGTAATGCAGCTG
AATTTGAATTAGAAAACCATGCTCGTCAGTATTCTGGTTCTATTCGCGATGATGGTTTACGTAAGGTGCTCTCAGGTAAA
ACTACACTTGAAGAAGTGCTTCGTGTAACCAATGAAGCAAGTGAAGCATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(118-384)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB V5VGM1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

41.598

98.387

0.409
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.057

99.194

0.407
  pilF Thermus thermophilus HB27

39.351

99.395

0.391
  pilB Vibrio campbellii strain DS40M4

48.371

80.444

0.389
  pilB/pilB1 Synechocystis sp. PCC 6803

40.041

97.177

0.389
  pilB Vibrio parahaemolyticus RIMD 2210633

48

80.645

0.387
  pilB Vibrio cholerae strain A1552

48

80.645

0.387
  pilF Neisseria gonorrhoeae MS11

48.477

79.435

0.385
  pilB Acinetobacter baumannii D1279779

47.146

81.25

0.383
  pilB Acinetobacter baylyi ADP1

46.787

78.427

0.367

Multiple sequence alignment