Detailed information NTG0389680

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: BACIH_RS12020
• Genome accession: NZ_CP044360
• Coordinates: 2458361..2458975 (-)
• Length: 204 a.a.
• NCBI ID: WP_012118026.1
• Uniprot ID: A7Z6X3
• Organism: Bacillus amyloliquefaciens strain V167
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2453361..2463975

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BACIH_RS11990 (BACIH_2447)	rpsT	2453664..2453930 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
BACIH_RS11995 (BACIH_2448)	holA	2453947..2454988 (-)	1042	Protein_2330	DNA polymerase III subunit delta	-
BACIH_RS12000	-	2455028..2455179 (-)	152	Protein_2331	hypothetical protein	-
BACIH_RS12005 (BACIH_2449)	-	2455220..2455354 (+)	135	WP_003152870.1	YqzM family protein	-
BACIH_RS12010 (BACIH_2450)	comEC	2455373..2457724 (-)	2352	WP_012118025.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
BACIH_RS12015 (BACIH_2451)	-	2457725..2458294 (-)	570	WP_003152868.1	ComE operon protein 2	-
BACIH_RS12020 (BACIH_2452)	comEA	2458361..2458975 (-)	615	WP_012118026.1	helix-hairpin-helix domain-containing protein	Machinery gene
BACIH_RS12025 (BACIH_2453)	comER	2459034..2459855 (+)	822	WP_012118027.1	late competence protein ComER	-
BACIH_RS12030 (BACIH_2454)	-	2459924..2460661 (-)	738	WP_012118028.1	class I SAM-dependent methyltransferase	-
BACIH_RS12035 (BACIH_2455)	rsfS	2460658..2461014 (-)	357	WP_007408260.1	ribosome silencing factor	-
BACIH_RS12040 (BACIH_2456)	yqeK	2461031..2461591 (-)	561	WP_012118029.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
BACIH_RS12045 (BACIH_2457)	-	2461581..2462150 (-)	570	WP_012118030.1	nicotinate-nucleotide adenylyltransferase	-
BACIH_RS12050 (BACIH_2458)	yhbY	2462161..2462451 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
BACIH_RS12055 (BACIH_2459)	aroE	2462445..2463287 (-)	843	WP_012118031.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22195.40 Da
• Isoelectric Point: 6.4888

>NTDB_id=389680 BACIH_RS12020 WP_012118026.1 2458361..2458975(-) (comEA) [Bacillus amyloliquefaciens strain V167]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINMASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=389680 BACIH_RS12020 WP_012118026.1 2458361..2458975(-) (comEA) [Bacillus amyloliquefaciens strain V167]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATATGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

3D structure

• AlphaFold DB: A7Z6X3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA	Latilactobacillus sakei subsp. sakei 23K	32.328	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363