Detailed information NTG0387463

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: FOB65_RS15190
• Genome accession: NZ_CP044074
• Coordinates: 2957051..2958745 (-)
• Length: 564 a.a.
• NCBI ID: WP_150339511.1
• Uniprot ID: -
• Organism: Pseudomonas oryzihabitans strain FDAARGOS_657
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	2955623..2973026	2957051..2958745	within	0

Gene organization within MGE regions

Location: 2955623..2973026

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FOB65_RS15185 (FOB65_15185)	pilC	2955623..2956840 (-)	1218	WP_027600839.1	type II secretion system F family protein	Machinery gene
FOB65_RS15190 (FOB65_15190)	pilB	2957051..2958745 (-)	1695	WP_150339511.1	type IV-A pilus assembly ATPase PilB	Machinery gene
FOB65_RS15195 (FOB65_15195)	-	2958958..2959422 (+)	465	WP_150339514.1	pilin	-
FOB65_RS15200 (FOB65_15200)	-	2959969..2960967 (-)	999	WP_150339517.1	SDR family oxidoreductase	-
FOB65_RS15210 (FOB65_15210)	cyoA	2961740..2962663 (+)	924	WP_007160924.1	ubiquinol oxidase subunit II	-
FOB65_RS15215 (FOB65_15215)	cyoB	2962667..2964655 (+)	1989	WP_007160923.1	cytochrome o ubiquinol oxidase subunit I	-
FOB65_RS15220 (FOB65_15220)	-	2964660..2965274 (+)	615	WP_027600197.1	cytochrome o ubiquinol oxidase subunit III	-
FOB65_RS15225 (FOB65_15225)	cyoD	2965274..2965612 (+)	339	WP_007160921.1	cytochrome o ubiquinol oxidase subunit IV	-
FOB65_RS15230 (FOB65_15230)	cyoE	2965624..2966511 (+)	888	WP_223855368.1	heme o synthase	-
FOB65_RS24505	-	2966579..2970496 (-)	3918	WP_223855369.1	pilus assembly protein	-
FOB65_RS15240 (FOB65_15240)	-	2970493..2971107 (-)	615	WP_150339519.1	PilX N-terminal domain-containing pilus assembly protein	-
FOB65_RS15245 (FOB65_15245)	-	2971104..2971892 (-)	789	WP_150339521.1	PilW family protein	-
FOB65_RS15250 (FOB65_15250)	pilV	2971896..2972492 (-)	597	WP_027600847.1	type IV pilus modification protein PilV	-
FOB65_RS15255 (FOB65_15255)	-	2972493..2973026 (-)	534	WP_027600848.1	GspH/FimT family pseudopilin	-

Sequence

Protein

• Length: 564 a.a.
• Molecular weight: 62405.75 Da
• Isoelectric Point: 5.7788

>NTDB_id=387463 FOB65_RS15190 WP_150339511.1 2957051..2958745(-) (pilB) [Pseudomonas oryzihabitans strain FDAARGOS_657]
MNETVMLSGLARQLVDAQLLAEAAAQQAVVKAKQAKVPLVTYLVQQRLVQSRPLAELAAQEFGVPFLDLAAIDKEQLPKD
IVNEKLMQQHHFLPLSKRGNKLFIALSDPTNQQAINDVSFGSKLAVEVVLVEDDKLHQAIERYLDTGTGGLDLGDIDLDL
EADGGERPQEAVAGTDADDAPVVKFVNKMLLDAIKKGSSDLHFEPYEKMYRVRFRTDGVLHEAARPPVQLVSRISARLKV
MSNMDISERRKPQDGRVKLKVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEDVQKELYLQALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTEDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNLATSVNLIIAQRLARKLCNKCKRVHEVPREALLAEGFREEE
IGSFTLYQPIGCEDCNAGYRGRVGIYEVVKITPALQQMIMEEGNSIEIAARMRKEGFNDLRRSGLVKAMQGITSLEEINR
VTKD

Nucleotide

• Length: 1695 bp

>NTDB_id=387463 FOB65_RS15190 WP_150339511.1 2957051..2958745(-) (pilB) [Pseudomonas oryzihabitans strain FDAARGOS_657]
ATGAACGAGACGGTGATGCTGTCGGGGCTGGCGCGGCAGTTGGTAGATGCTCAGCTGCTGGCGGAGGCGGCAGCGCAGCA
GGCGGTGGTCAAGGCCAAGCAGGCCAAGGTGCCACTGGTGACCTATCTCGTTCAGCAGCGGCTGGTGCAAAGCCGCCCTT
TGGCGGAGTTGGCTGCCCAGGAATTCGGCGTACCCTTTCTTGATCTTGCGGCTATCGACAAGGAGCAGCTACCCAAGGAC
ATCGTCAACGAGAAGCTGATGCAGCAGCATCACTTCCTGCCTTTGAGCAAAAGGGGCAACAAGCTTTTCATCGCGCTTTC
CGATCCTACTAACCAGCAGGCCATCAATGATGTGTCCTTTGGCAGCAAGCTGGCCGTCGAGGTCGTGCTGGTCGAGGACG
ACAAGCTGCACCAGGCCATCGAGCGATATCTGGACACCGGCACGGGTGGCCTGGACCTAGGCGATATCGATCTTGATCTG
GAAGCCGATGGCGGCGAGCGGCCTCAGGAAGCCGTTGCGGGTACCGACGCTGACGATGCGCCCGTGGTTAAGTTCGTCAA
TAAGATGCTGCTCGACGCTATCAAGAAGGGTTCGTCCGACCTGCACTTCGAGCCCTATGAAAAGATGTATCGGGTACGCT
TCCGTACCGACGGCGTCTTGCACGAGGCGGCGCGGCCGCCCGTGCAGCTGGTGTCGCGGATCTCGGCACGGCTCAAGGTG
ATGTCCAATATGGATATCTCCGAGCGGCGCAAACCTCAGGATGGCCGGGTCAAGCTCAAGGTGTCCAAGACCAAGTCCAT
CGACTTCCGGGTCAATACCTTGCCCACCCTCTGGGGCGAAAAGATCGTGATGCGGATTCTGGATTCCTCTAGCGCCCAGA
TGGGGATCGATGCCTTGGGCTACGAGGATGTGCAGAAGGAGCTCTATCTGCAGGCACTCAAGCAGCCGCAGGGGATGATC
CTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTATCCCTCTATACCGGCCTCAACATCCTCAATACCGAAGACATCAA
TATTTCCACGGCGGAGGATCCGGTGGAAATCAACTTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGTCAGGGGCTCG
ACTTCTCCCAGGCGCTGCGGGCCTTCCTGCGTCAGGATCCTGACGTGATCATGGTTGGTGAGATCCGCGACCTGGAAACG
GCGGAGATTGCCATCAAGGCAGCCCAGACCGGTCACATGGTGATGTCGACCCTGCACACCAACAGCGCGGCGGAAACCCT
AACCCGTCTACGCAACATGGGGGTGCCTGCCTTCAACCTGGCGACCTCGGTCAACCTCATCATCGCCCAGCGGCTGGCGC
GCAAGCTGTGCAACAAGTGCAAGCGAGTCCATGAGGTGCCCCGTGAGGCGCTGCTGGCCGAAGGCTTTCGCGAGGAAGAA
ATCGGCAGTTTCACCCTTTATCAGCCTATCGGCTGCGAGGACTGCAATGCGGGCTATCGCGGGCGGGTAGGGATCTACGA
GGTGGTGAAGATCACCCCGGCACTGCAACAGATGATCATGGAGGAGGGCAACTCCATCGAGATCGCGGCGCGGATGCGCA
AAGAGGGCTTCAATGATCTGCGGCGTTCGGGACTGGTGAAGGCGATGCAGGGGATTACCAGTCTGGAAGAGATCAATCGG
GTCACCAAGGACTAG

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-146)

• Type II/IV secretion system protein (T2SSE) (188-455)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	60.142	99.645	0.599
pilB	Acinetobacter baylyi ADP1	60.071	99.468	0.598
pilB	Legionella pneumophila strain ERS1305867	54.737	100	0.553
pilB	Vibrio cholerae strain A1552	50.442	100	0.505
pilB	Vibrio campbellii strain DS40M4	49.911	99.823	0.498
pilF	Neisseria gonorrhoeae MS11	49.201	99.823	0.491
pilB	Vibrio parahaemolyticus RIMD 2210633	49.376	99.468	0.491
pilF	Thermus thermophilus HB27	36.786	99.291	0.365
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.857	93.085	0.362