Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FVA82_RS26765 Genome accession   NZ_CP044006
Coordinates   5551256..5552956 (+) Length   566 a.a.
NCBI ID   WP_003107297.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain E90     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 5532026..5551025 5551256..5552956 flank 231


Gene organization within MGE regions


Location: 5532026..5552956
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FVA82_RS26615 (FVA82_26615) - 5532026..5532742 (-) 717 WP_073624880.1 hypothetical protein -
  FVA82_RS26620 (FVA82_26620) - 5532891..5533091 (+) 201 WP_023435053.1 helix-turn-helix domain-containing protein -
  FVA82_RS26625 (FVA82_26625) - 5533088..5533504 (+) 417 WP_031689984.1 hypothetical protein -
  FVA82_RS26630 (FVA82_26630) - 5533497..5533730 (+) 234 WP_023110507.1 hypothetical protein -
  FVA82_RS26635 (FVA82_26635) - 5533727..5533996 (+) 270 WP_031689985.1 hypothetical protein -
  FVA82_RS26640 (FVA82_26640) - 5533993..5534295 (+) 303 WP_031689987.1 hypothetical protein -
  FVA82_RS26645 (FVA82_26645) - 5534298..5534588 (+) 291 WP_023082013.1 hypothetical protein -
  FVA82_RS26650 (FVA82_26650) - 5534585..5534959 (+) 375 WP_023109347.1 hypothetical protein -
  FVA82_RS26655 (FVA82_26655) - 5534956..5536506 (+) 1551 WP_031689988.1 DUF3631 domain-containing protein -
  FVA82_RS26660 (FVA82_26660) - 5536506..5536802 (+) 297 WP_003096128.1 hypothetical protein -
  FVA82_RS26665 (FVA82_26665) - 5537063..5537881 (+) 819 WP_031689989.1 hypothetical protein -
  FVA82_RS26670 (FVA82_26670) - 5537986..5538285 (+) 300 WP_031689990.1 hypothetical protein -
  FVA82_RS26675 (FVA82_26675) - 5539158..5539664 (-) 507 WP_003096122.1 GNAT family N-acetyltransferase -
  FVA82_RS26685 (FVA82_26685) - 5540416..5540685 (+) 270 WP_003096118.1 hypothetical protein -
  FVA82_RS26690 (FVA82_26690) - 5540685..5540921 (+) 237 WP_023082019.1 hypothetical protein -
  FVA82_RS26695 (FVA82_26695) - 5541128..5541409 (-) 282 WP_049951334.1 hypothetical protein -
  FVA82_RS26700 (FVA82_26700) - 5541606..5542304 (+) 699 WP_124132301.1 hypothetical protein -
  FVA82_RS26705 (FVA82_26705) - 5542329..5543333 (+) 1005 WP_031689993.1 pesticin C-terminus-like muramidase -
  FVA82_RS26710 (FVA82_26710) - 5543346..5544008 (+) 663 WP_023082023.1 DUF3218 family protein -
  FVA82_RS26720 (FVA82_26720) fliP 5544291..5545043 (+) 753 WP_150468667.1 flagellar type III secretion system pore protein FliP -
  FVA82_RS26725 (FVA82_26725) fliQ 5545088..5545357 (+) 270 WP_023435365.1 flagellar biosynthesis protein FliQ -
  FVA82_RS26730 (FVA82_26730) fliR 5545357..5546133 (+) 777 Protein_5174 flagellar biosynthetic protein FliR -
  FVA82_RS26735 (FVA82_26735) - 5546136..5546395 (+) 260 Protein_5175 EscU/YscU/HrcU family type III secretion system export apparatus switch protein -
  FVA82_RS26740 (FVA82_26740) - 5546472..5547559 (+) 1088 WP_223882067.1 IS3 family transposase -
  FVA82_RS26745 (FVA82_26745) - 5548589..5548831 (+) 243 WP_031690159.1 hypothetical protein -
  FVA82_RS26755 (FVA82_26755) - 5549090..5550403 (-) 1314 WP_223218619.1 O-antigen ligase family protein -
  FVA82_RS26760 (FVA82_26760) - 5550561..5551025 (-) 465 WP_023086642.1 pilin -
  FVA82_RS26765 (FVA82_26765) pilB 5551256..5552956 (+) 1701 WP_003107297.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62312.21 Da        Isoelectric Point: 5.4470

>NTDB_id=387003 FVA82_RS26765 WP_003107297.1 5551256..5552956(+) (pilB) [Pseudomonas aeruginosa strain E90]
MNDSTPLSGLAKQLVLNELLDEKTARQAQVQAARNKQSLVSHLVQAKLVQSRALVEVAAEQFGIAYCDLNSLDRESIPKD
VISERLVRQHRVLPLWKRGNKLFIGLSDPTNHQAVTDIQFSTGLTTEALLVEDDKLGVAIEKLYEGAMDALGDLGDIDLD
GVDVGKAESASQDDGGDDAEDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKTYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKNKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
ELIGTFKLYSPVGCENCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=387003 FVA82_RS26765 WP_003107297.1 5551256..5552956(+) (pilB) [Pseudomonas aeruginosa strain E90]
ATGAACGATTCCACGCCTCTCAGCGGCCTTGCCAAGCAACTGGTCCTGAACGAACTCCTCGACGAAAAGACCGCCCGCCA
GGCCCAGGTGCAGGCCGCGCGCAACAAGCAGTCGCTGGTCAGCCACCTGGTCCAGGCCAAGCTGGTGCAGAGCCGGGCGC
TGGTCGAGGTGGCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGATCGGGAAAGCATTCCGAAGGAT
GTCATCAGCGAGCGCCTGGTCCGCCAGCACCGCGTGTTGCCGCTGTGGAAACGCGGCAACAAACTGTTCATCGGTCTCTC
CGACCCGACCAACCATCAGGCGGTCACCGATATCCAGTTCAGCACCGGCCTCACCACCGAAGCCCTGCTGGTAGAGGACG
ACAAGCTCGGCGTAGCCATCGAGAAGCTTTACGAGGGCGCCATGGACGCCCTCGGCGACCTCGGCGATATCGATCTCGAC
GGGGTCGATGTCGGCAAGGCGGAAAGCGCCAGCCAGGACGACGGCGGCGACGATGCCGAAGACGCCCCCGTGGTGCGCTT
CGTCAACAAGATGCTGCTCGACGCCATCCGCGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAAAAGACCTATCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGTCGTATCTCTGCTCGTCTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGACGCAAACCGCAGGACGGACGGATCAAGATGCGCGTATCGAAGAACAA
GTCGATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGACGCACTGGGCTACGAAGAGGACCAGAAGGAACTCTACCTGGCCGCGCTCAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTTTCGCTGTACACCGGTCTGAACATCCTCAATACCACCGA
CATCAACATCTCCACCGCTGAAGACCCTGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGATCTG
GAGACTGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGCCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGACGTGCCGAAGGAAACCCTGCTTCACGAAGGTTTCCCCGAA
GAGCTGATCGGCACCTTCAAGCTGTATTCGCCTGTAGGCTGCGAAAACTGCAAAGGCGGTTACAAAGGTCGCGTCGGTAT
TTATGAAGTGGTTAAAAACACCCCCGCCCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGAATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.544

100

0.58
  pilB Acinetobacter baylyi ADP1

56.79

100

0.569
  pilB Legionella pneumophila strain ERS1305867

54.497

100

0.546
  pilB Vibrio cholerae strain A1552

51.521

98.763

0.509
  pilF Neisseria gonorrhoeae MS11

50.177

99.647

0.5
  pilB Vibrio parahaemolyticus RIMD 2210633

52.158

94.17

0.491
  pilB Vibrio campbellii strain DS40M4

51.22

94.17

0.482
  pilB/pilB1 Synechocystis sp. PCC 6803

37.763

100

0.412
  pilF Thermus thermophilus HB27

38.664

100

0.389
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.727

97.173

0.376

Multiple sequence alignment