Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   F0Q04_RS21520 Genome accession   NZ_CP043575
Coordinates   4699024..4700757 (+) Length   577 a.a.
NCBI ID   WP_116927081.1    Uniprot ID   A0A363RHC4
Organism   Comamonas koreensis strain T50-37     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4694024..4705757
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F0Q04_RS21495 proB 4694545..4695684 (-) 1140 WP_116927078.1 glutamate 5-kinase -
  F0Q04_RS21500 obgE 4695769..4696884 (-) 1116 WP_116927079.1 Obg family GTPase CgtA -
  F0Q04_RS21505 rpmA 4697074..4697334 (-) 261 WP_021027814.1 50S ribosomal protein L27 -
  F0Q04_RS21510 rplU 4697350..4697661 (-) 312 WP_027011098.1 50S ribosomal protein L21 -
  F0Q04_RS21515 - 4697929..4698858 (+) 930 WP_182345844.1 polyprenyl synthetase family protein -
  F0Q04_RS21520 pilB 4699024..4700757 (+) 1734 WP_116927081.1 type IV-A pilus assembly ATPase PilB Machinery gene
  F0Q04_RS21525 pilC 4700778..4701998 (+) 1221 WP_116927082.1 type II secretion system F family protein Machinery gene
  F0Q04_RS21530 - 4702001..4702876 (+) 876 WP_182343457.1 prepilin peptidase -
  F0Q04_RS21535 coaE 4702878..4703507 (+) 630 WP_182343459.1 dephospho-CoA kinase -
  F0Q04_RS21540 zapD 4703511..4704266 (+) 756 WP_182343461.1 cell division protein ZapD -
  F0Q04_RS21545 - 4704328..4704540 (+) 213 WP_116927086.1 DNA gyrase inhibitor YacG -
  F0Q04_RS21550 - 4704588..4705061 (-) 474 WP_182343463.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62664.07 Da        Isoelectric Point: 5.2339

>NTDB_id=385258 F0Q04_RS21520 WP_116927081.1 4699024..4700757(+) (pilB) [Comamonas koreensis strain T50-37]
MPDINTPPKAVALPGIARALVASGKLPVAKAEALYATSLKSKNSFIGELVASGAVSGSDVASIISTVYGAPLVDLDAIDL
TRLATGLLDPKLALSNKIIPLAKRGNRLIVVTADPTDHSVAEQIKFSTQLSVDWVIAEHSKLVKALESVAKKGGDNIQDM
ITGDVNLNEFEIDDESVNTDTASNDGDEVEDAPIVKFLQKMLIDAFNMRASDLHFEPYEHQYRIRFRIDGELREIASPPP
GIKDKLASRIKVISRLDISEKRVPQDGRMKLKIGADRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPEEKER
LLSAIKRPYGMILVTGPTGSGKTVSLYTCLNILNTPAVNIATAEDPSEINLPGVNQVNVNEKAGLTFATALKSFLRQDPD
IIMVGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARKLCPICKRPTEIPH
EALLEAGFKEEDLDGTWMPYEPVGCDACNNGYKGRLGIYQVMPISEETQRIILRDGSALEIAEQADIENVRSLRTSGLHK
VKKGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=385258 F0Q04_RS21520 WP_116927081.1 4699024..4700757(+) (pilB) [Comamonas koreensis strain T50-37]
ATGCCTGACATCAACACCCCCCCCAAAGCCGTCGCCCTCCCCGGCATTGCCAGAGCGCTGGTAGCCAGTGGAAAGCTTCC
TGTTGCCAAGGCGGAGGCCTTGTACGCCACCAGTCTCAAAAGCAAGAACAGCTTCATTGGAGAGCTGGTGGCCAGTGGTG
CCGTCTCCGGCAGCGATGTAGCCAGCATCATCTCGACGGTCTATGGCGCGCCGCTGGTCGACCTCGATGCTATCGATTTG
ACCCGGCTGGCAACAGGTTTGCTTGATCCCAAACTGGCGCTCAGCAACAAGATCATTCCCCTGGCCAAACGCGGCAACCG
GCTGATCGTCGTTACCGCCGACCCGACCGACCACTCGGTGGCCGAGCAGATCAAGTTCTCCACCCAGCTGAGCGTGGACT
GGGTGATTGCAGAACATTCCAAGCTGGTCAAGGCGCTGGAGTCTGTTGCGAAAAAGGGCGGCGACAACATCCAGGACATG
ATCACCGGCGATGTCAACCTCAATGAATTCGAGATTGATGATGAGTCGGTGAACACCGACACCGCGAGCAATGACGGAGA
TGAGGTCGAAGATGCGCCCATCGTCAAGTTTTTGCAGAAGATGCTGATTGACGCATTCAATATGCGTGCATCGGACTTGC
ATTTCGAACCCTATGAGCACCAGTACCGCATCCGCTTTCGCATCGATGGAGAGCTGCGCGAAATCGCTTCGCCGCCCCCG
GGCATCAAGGACAAGCTGGCATCGCGGATCAAGGTGATCTCGCGCCTGGATATCTCGGAAAAGCGCGTGCCGCAGGACGG
CCGCATGAAGCTCAAGATCGGCGCGGATCGGGTGATCGATTTCCGGGTCAGCACCTTGCCCACCTTGTTTGGCGAAAAGA
TCGTGATCCGTATTCTCGATCCGAGCAGCGCCAAGCTGGGCATTGATGCACTGGGCTATGAGCCCGAAGAGAAGGAGCGC
CTGCTCAGCGCCATCAAGCGCCCGTACGGCATGATTCTGGTCACGGGGCCCACGGGTTCGGGCAAGACCGTGTCGCTCTA
CACCTGCCTGAATATTCTCAATACGCCAGCGGTCAATATAGCGACTGCCGAAGACCCGTCGGAAATCAACCTGCCCGGGG
TCAACCAGGTCAATGTCAATGAAAAGGCCGGTCTGACCTTTGCGACCGCGCTCAAGTCCTTTCTGCGCCAGGATCCCGAC
ATCATCATGGTCGGTGAAATCCGTGACCTGGAGACGGCCGACATCGCCATCAAGGCGGCGCAGACCGGTCACTTGGTGCT
GTCGACCCTGCACACCAACGACGCCCCCACCACCTTGACGCGTATGCGCAATATGGGGATCGCGCCCTTCAATATCGCGT
CGAGCGTGATCTTGATCACCGCGCAGCGCCTGGCACGCAAGCTCTGCCCGATCTGCAAGCGCCCCACCGAAATCCCCCAT
GAAGCGCTGCTGGAAGCGGGCTTCAAGGAAGAGGACCTGGATGGCACCTGGATGCCCTACGAGCCCGTTGGCTGCGACGC
CTGCAACAATGGCTACAAGGGCCGCCTGGGCATCTACCAAGTGATGCCCATCTCCGAAGAAACACAGCGCATCATCTTGC
GCGATGGCAGCGCACTGGAAATCGCCGAGCAAGCCGACATAGAAAATGTCCGCTCCTTGCGCACGTCAGGCCTGCACAAA
GTCAAAAAGGGACTGACCTCACTCGAAGAGGTCTTAGCCGTCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A363RHC4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.75

100

0.549
  pilB Acinetobacter baumannii D1279779

54.202

100

0.548
  pilF Neisseria gonorrhoeae MS11

53.004

98.094

0.52
  pilB Legionella pneumophila strain ERS1305867

50.265

98.267

0.494
  pilB Vibrio cholerae strain A1552

46.032

98.267

0.452
  pilB Vibrio campbellii strain DS40M4

45.439

98.787

0.449
  pilB Vibrio parahaemolyticus RIMD 2210633

45.633

97.227

0.444
  pilF Thermus thermophilus HB27

38.879

92.721

0.36

Multiple sequence alignment