Detailed information NTG0384913

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: F0H32_RS15125
• Genome accession: NZ_CP043540
• Coordinates: 3525886..3527622 (-)
• Length: 578 a.a.
• NCBI ID: WP_058362148.1
• Uniprot ID: -
• Organism: Xanthomonas translucens pv. undulosa strain LW16
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	3515270..3528781	3525886..3527622	within	0

Gene organization within MGE regions

Location: 3515270..3528781

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
F0H32_RS15085 (F0H32_15085)	-	3515270..3517564 (+)	2295	WP_139115860.1	hypothetical protein	-
F0H32_RS21260	-	3517677..3517982 (-)	306	WP_003466368.1	PQQ-binding-like beta-propeller repeat protein	-
F0H32_RS21265	-	3517931..3519229 (-)	1299	WP_230938577.1	hypothetical protein	-
F0H32_RS15095 (F0H32_15095)	sucD	3519515..3520387 (-)	873	WP_003466372.1	succinate--CoA ligase subunit alpha	-
F0H32_RS15100 (F0H32_15100)	sucC	3520411..3521574 (-)	1164	WP_003466374.1	ADP-forming succinate--CoA ligase subunit beta	-
F0H32_RS15105 (F0H32_15105)	-	3521815..3523428 (+)	1614	WP_003466376.1	ATP-binding protein	-
F0H32_RS15110 (F0H32_15110)	pilR	3523503..3524891 (+)	1389	WP_058362147.1	sigma-54 dependent transcriptional regulator	Regulator
F0H32_RS15115 (F0H32_15115)	-	3525080..3525262 (+)	183	WP_003466380.1	YhfG family protein	-
F0H32_RS15120 (F0H32_15120)	-	3525259..3525711 (+)	453	WP_230811680.1	Fic family protein	-
F0H32_RS15125 (F0H32_15125)	pilB	3525886..3527622 (-)	1737	WP_058362148.1	type IV-A pilus assembly ATPase PilB	Machinery gene
F0H32_RS15130 (F0H32_15130)	-	3527810..3528115 (+)	306	WP_230938578.1	hypothetical protein	-
F0H32_RS15135 (F0H32_15135)	-	3528371..3528781 (-)	411	WP_058362150.1	pilin	-

Sequence

Protein

• Length: 578 a.a.
• Molecular weight: 62873.16 Da
• Isoelectric Point: 6.3627

>NTDB_id=384913 F0H32_RS15125 WP_058362148.1 3525886..3527622(-) (pilB) [Xanthomonas translucens pv. undulosa strain LW16]
MNAVTSANLVGITGIARRLVQDGALEETAARTAMAHAAEAKIPLPQWFSDKKLVTAAQLAAANALEFGMPLLDVSVFDAS
QSAMKLVSEELLHKHQVLPLFKRGNRLFVGMSNPTQTRALDDIKFHTNLTVEPILVDEDQIRRTLEQWQASNDAIGDALG
DDDEAMGKLEVGAGDEDMGSGGDTGVDAKGDDTPVVKFVNKMLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKSVARAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKSKQIDFRVSTLPTLFGEKIVLRVLDGSAAKLGIDKLGYEPEQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAVALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPTHLP
HNALLAEGFSEAEVAAGIQLYEAVGCDECTEGYKGRTGIYQVMPMNDEIAAIVLQGGNAMDIAQAAQKIGVKDLRQSALL
KARAGITSLAEINRVTKD

Nucleotide

• Length: 1737 bp

>NTDB_id=384913 F0H32_RS15125 WP_058362148.1 3525886..3527622(-) (pilB) [Xanthomonas translucens pv. undulosa strain LW16]
ATGAACGCTGTGACATCCGCCAATCTTGTCGGCATCACCGGCATCGCGCGCCGCCTGGTTCAGGATGGCGCGCTCGAGGA
AACGGCTGCGCGGACCGCGATGGCGCATGCCGCCGAGGCCAAGATCCCGCTGCCGCAGTGGTTCTCGGACAAGAAGCTGG
TCACCGCGGCGCAGCTGGCCGCCGCCAATGCGCTGGAGTTCGGGATGCCGTTGCTGGACGTGTCGGTGTTCGATGCCAGC
CAGAGCGCGATGAAGCTGGTCAGCGAGGAGCTGTTGCACAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGTCT
GTTCGTCGGGATGAGCAATCCGACCCAGACCCGGGCGCTGGACGATATCAAGTTCCATACCAATTTGACGGTCGAGCCGA
TCCTGGTCGATGAGGACCAGATCCGCCGCACCCTGGAACAGTGGCAGGCGAGCAACGATGCGATCGGCGACGCGCTGGGC
GACGACGACGAGGCTATGGGCAAGCTCGAGGTCGGTGCCGGCGACGAGGACATGGGCAGCGGCGGCGATACCGGCGTCGA
TGCCAAGGGCGACGATACGCCCGTGGTCAAGTTCGTGAACAAGATGCTGGTCGATGCGATCCGCCGCGGCGCGTCGGACA
TCCATTTCGAGCCGTACGAGGACGATTACCGGGTGCGCCTGCGCATCGACGGCCTGCTCAAGAGCGTGGCCAGGGCGCCG
GTCAAGCTCAACCAGCGCATTGCGGCGCGGCTCAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGA
CGGACGCATCAAGCTCAACCTGTCCAAGAGCAAGCAGATCGATTTCCGCGTCAGCACGCTGCCGACCTTGTTCGGCGAGA
AGATCGTGCTGCGTGTCCTCGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTCGGCTACGAGCCGGAGCAGCAGAAG
CTGTTCCTGGACGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCT
GTACACCGCGCTGGGCATCCTCAATGACGAGACCCGCAACATCTCCACCGCCGAGGATCCGGTGGAAATCCGCCTGCCCG
GGGTCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGTGGCGCTGCGCTCTTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAGATCCGCGACCTGGAAACCGCCGAGATCGCGATCAAGGCGGCGCAGACCGGGCACATGGT
GCTGTCCACGCTGCACACCAACGATGCGCCGCAGACCATCGCGCGGCTGATGAACATGGGGATCGCGCCGTACAACATCA
CCAGTTCGGTGACGCTGGTGATCGCGCAGCGTCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCCCGACCCATCTGCCG
CACAATGCGCTGCTGGCCGAGGGTTTCAGCGAGGCGGAAGTGGCCGCCGGCATCCAGCTGTACGAGGCGGTGGGCTGCGA
CGAGTGCACCGAGGGCTACAAGGGCCGCACCGGCATCTATCAGGTGATGCCGATGAACGACGAGATCGCGGCGATCGTGC
TGCAGGGCGGCAACGCGATGGACATCGCCCAGGCCGCGCAGAAGATCGGGGTCAAGGACCTGCGCCAGTCGGCGCTGCTC
AAGGCGCGCGCCGGCATCACCAGCCTGGCGGAGATCAATCGCGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (201-468)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (51-152)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baylyi ADP1	54.754	98.27	0.538
pilB	Acinetobacter baumannii D1279779	54.101	99.135	0.536
pilB	Legionella pneumophila strain ERS1305867	52.548	98.443	0.517
pilB	Vibrio cholerae strain A1552	48.873	99.827	0.488
pilF	Neisseria gonorrhoeae MS11	48.592	98.27	0.478
pilB	Vibrio parahaemolyticus RIMD 2210633	46.996	97.924	0.46
pilB	Vibrio campbellii strain DS40M4	45.518	98.443	0.448
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.23	90.311	0.363
pilF	Thermus thermophilus HB27	40.192	89.965	0.362