Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FYA21_RS05525 Genome accession   NZ_CP043180
Coordinates   1134070..1135782 (-) Length   570 a.a.
NCBI ID   WP_148745960.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain PG20180064     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1129070..1140782
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FYA21_RS05495 (FYA21_05495) - 1129232..1129558 (+) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -
  FYA21_RS05500 (FYA21_05500) rlmB 1129660..1130409 (+) 750 WP_001118149.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  FYA21_RS05505 (FYA21_05505) - 1130445..1131359 (+) 915 WP_096640131.1 DMT family transporter -
  FYA21_RS05510 (FYA21_05510) coaE 1131356..1131952 (-) 597 WP_000874125.1 dephospho-CoA kinase -
  FYA21_RS18800 (FYA21_05515) pilD 1131954..1132814 (-) 861 WP_096640130.1 prepilin peptidase Machinery gene
  FYA21_RS05520 (FYA21_05520) pilC 1132814..1134040 (-) 1227 WP_000279216.1 type II secretion system F family protein Machinery gene
  FYA21_RS05525 (FYA21_05525) pilB 1134070..1135782 (-) 1713 WP_148745960.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FYA21_RS05530 (FYA21_05530) tpiA 1136073..1136867 (+) 795 WP_000016939.1 triose-phosphate isomerase -
  FYA21_RS05535 (FYA21_05535) secG 1136880..1137209 (+) 330 WP_000555570.1 preprotein translocase subunit SecG -
  FYA21_RS05555 (FYA21_05555) rimP 1137851..1138375 (+) 525 WP_000777730.1 ribosome maturation factor RimP -
  FYA21_RS05560 (FYA21_05560) nusA 1138413..1139897 (+) 1485 WP_000532248.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63310.67 Da        Isoelectric Point: 5.2371

>NTDB_id=382012 FYA21_RS05525 WP_148745960.1 1134070..1135782(-) (pilB) [Acinetobacter baumannii strain PG20180064]
MSALHTSPKFTGFFRRLVEEKHVSGATMQTALDAAKRAKQDTVAYLIEEVHLSPSLLAETISAEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLLVEPVIVEHHKLDKVLGQHFAEESSFDFNDEEF
DLDVNLDGPTAPEDEEEAPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKIPADIPKQSLLEMGF
TEQDLAHPDFRVFQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=382012 FYA21_RS05525 WP_148745960.1 1134070..1135782(-) (pilB) [Acinetobacter baumannii strain PG20180064]
ATGTCAGCATTACATACATCTCCAAAATTTACGGGGTTTTTTCGACGTTTAGTCGAAGAAAAGCATGTGTCGGGAGCGAC
CATGCAAACTGCATTAGATGCAGCGAAAAGAGCTAAGCAGGACACAGTCGCATATTTAATTGAAGAGGTTCATCTCTCCC
CTTCGTTATTGGCTGAAACAATTTCTGCTGAGTTTGCTGAACCATATTTCGATCTTGATGTTTATGACACCTCTCAGATT
CCTAAAGATCTGGTCGATCAGAAACTGATTTTAAAACATCGAATTTTGCCACTCATTCAAAGAGGGCAAATTTTATATGT
TGCGACCAGTAATCCAAGCAATATCGAAGCGATTGATGCAATCCGTTTTAATAGTAAATTGCTGGTTGAGCCTGTAATTG
TGGAGCACCATAAACTCGATAAAGTTTTAGGCCAGCATTTTGCAGAAGAAAGCAGCTTTGACTTCAATGATGAAGAGTTT
GATCTCGATGTTAATCTTGATGGTCCTACCGCTCCAGAAGATGAAGAAGAAGCTCCTCAAGGCGATGAAGCCCCGATTGT
TAAATATATTAATAAGTTATTAATTGATGCCATTCGTATGGGTGCTTCAGACTTACATTTTGAACCGTACGAAAAATCCT
ATCGGGTGCGCTATCGTGTCGATGGTGTGTTACGTCAAATTGCCAATCCCCCTCTACAGCTTGCAAACCGCTTGGCATCA
CGCTTAAAAGTAATGTCTCAAATGGACATTTCTGAAAAACGCGTCCCACAAGATGGTCGTATCAAGCTCAAGCTGTCAAA
AACCAAAGCGATTGATTTTCGTGTGAACTCCCTCCCGACTTTGTTTGGTGAAAAGTTAGTTCTACGTATTCTTGACCCAT
CAAGCGCAATGCTAGGAATTGATGCACTAGGATATGAAGAAGATCAAAAAGCTTTGTTTATGGAGGCATTAGATAAACCT
CAAGGCATGCTTTTAATTACCGGACCAACAGGTTCTGGTAAAACCGTATCGTTATATACAGGTTTAAATATTTTAAATAC
TGAAAGCTCCAATATTTCTACTGCCGAAGACCCGGTAGAAATTAACCTTGAAGGAATTAATCAGGTTAACGTAAACCCCA
AGGTAGGACTTACTTTTGCGGCCGCACTTAAATCATTTTTACGACAAGACCCTGACATTATTATGGTGGGTGAGATTCGT
GACTTGGAAACAGCAGAAATTGCGATTAAGGCGGCTCAAACCGGCCACATGGTGATGTCAACACTACATACTAATAGTGC
TCCGGAAACATTGACCCGTCTACGCAATATGGGGGTCCCTTCTTTTAATATTGCAACATCAGTAAACCTAGTTATTGCAC
AGCGTTTAGCACGTCGTCTTTGTTCTCAATGCAAAATACCTGCTGATATTCCTAAACAAAGCTTATTAGAAATGGGCTTT
ACCGAACAAGATTTAGCGCACCCAGATTTTCGGGTTTTTCAGCCCGTAGGTTGCCCTGAATGTCGTGAAGGCTATAAAGG
CCGTGTCGGTATTTATGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAACGCTTTAGAAA
TCGCAGCTGCTTCTGAAAAATTGGGGTTTAATAATCTACGTCGTTCAGGTTTAAAAAAGGTGATGCAAGGGGTGACCTCT
TTACAAGAAGTCAACCGTGTCACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

99.123

100

0.991
  pilB Acinetobacter baylyi ADP1

79.298

100

0.793
  pilF Neisseria gonorrhoeae MS11

50.356

98.596

0.496
  pilB Legionella pneumophila strain ERS1305867

52.731

93.158

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

50.089

98.07

0.491
  pilB Vibrio campbellii strain DS40M4

50.867

91.053

0.463
  pilB Vibrio cholerae strain A1552

50.29

90.702

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

34.853

100

0.375

Multiple sequence alignment