Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FRD49_RS16850 Genome accession   NZ_CP042994
Coordinates   3514866..3516578 (+) Length   570 a.a.
NCBI ID   WP_004710829.1    Uniprot ID   K9BAJ6
Organism   Acinetobacter nosocomialis strain J1A     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3509866..3521578
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FRD49_RS16815 (FRD49_16815) nusA 3510756..3512240 (-) 1485 WP_002156952.1 transcription termination factor NusA -
  FRD49_RS16820 (FRD49_16820) rimP 3512278..3512802 (-) 525 WP_032009947.1 ribosome maturation factor RimP -
  FRD49_RS16840 (FRD49_16840) secG 3513439..3513768 (-) 330 WP_002156913.1 preprotein translocase subunit SecG -
  FRD49_RS16845 (FRD49_16845) tpiA 3513781..3514575 (-) 795 WP_004710827.1 triose-phosphate isomerase -
  FRD49_RS16850 (FRD49_16850) pilB 3514866..3516578 (+) 1713 WP_004710829.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FRD49_RS16855 (FRD49_16855) pilC 3516606..3517832 (+) 1227 WP_004710832.1 type II secretion system F family protein Machinery gene
  FRD49_RS19275 (FRD49_16860) pilD 3517832..3518692 (+) 861 WP_004710834.1 prepilin peptidase Machinery gene
  FRD49_RS16865 (FRD49_16865) coaE 3518694..3519290 (+) 597 WP_000874125.1 dephospho-CoA kinase -
  FRD49_RS16870 (FRD49_16870) - 3519287..3520201 (-) 915 WP_006580951.1 DMT family transporter -
  FRD49_RS16875 (FRD49_16875) rlmB 3520237..3520986 (-) 750 WP_016803977.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  FRD49_RS16880 (FRD49_16880) - 3521088..3521414 (-) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63457.85 Da        Isoelectric Point: 5.2413

>NTDB_id=380054 FRD49_RS16850 WP_004710829.1 3514866..3516578(+) (pilB) [Acinetobacter nosocomialis strain J1A]
MSVLHTSPKFTGFFRRLVEEKHVSAATMQNALDAAKRAKQDTVAYLIEEVHLSPSLLAETISAEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLLVEPVIVEHHKLEKVLGQHFAEESSFDFNDEEF
DLDVSLDGPTTQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKIPADIPKQSLLEMGF
TEQDLAHPDFRVFQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=380054 FRD49_RS16850 WP_004710829.1 3514866..3516578(+) (pilB) [Acinetobacter nosocomialis strain J1A]
ATGTCAGTATTACATACATCTCCAAAATTTACGGGGTTTTTTCGGCGTTTAGTCGAAGAAAAGCATGTGTCGGCAGCGAC
CATGCAAAATGCATTAGATGCAGCAAAGAGAGCCAAGCAGGATACAGTCGCATATTTAATTGAAGAGGTACATCTCTCCC
CTTCTTTATTGGCTGAAACCATTTCTGCGGAGTTTGCTGAACCATATTTTGATCTAGATGTTTATGACACATCTCAGATT
CCTAAAGATCTGGTCGATCAAAAACTGATTTTAAAACATCGAATTTTGCCGCTCATTCAAAGAGGACAAATTCTATATGT
CGCCACCAGCAATCCAAGTAATATTGAAGCAATCGATGCAATCCGTTTTAACAGTAAATTGCTGGTTGAACCTGTTATTG
TCGAGCATCATAAGCTGGAAAAAGTTTTAGGCCAGCATTTTGCAGAAGAAAGCAGCTTTGACTTTAATGATGAAGAGTTT
GATCTTGACGTCAGCCTTGATGGTCCTACCACTCAAGAAGATGAAGAAGAAACACCTCAAGGTGATGAAGCTCCTATTGT
TAAATATATTAATAAGTTGCTTATTGATGCCATTCGTATGGGCGCATCAGATTTACATTTTGAGCCTTATGAAAAGTCAT
ATCGGGTACGTTATCGTGTCGATGGTGTGTTACGTCAAATTGCCAATCCGCCTTTACAACTTGCAAACCGCTTGGCATCA
CGTTTAAAAGTAATGTCTCAAATGGACATTTCCGAAAAACGTGTCCCACAAGATGGTCGTATCAAACTTAAGCTGTCAAA
AACCAAAGCAATTGATTTCCGTGTGAACTCGCTCCCTACCTTGTTTGGTGAAAAGTTAGTTCTACGTATTCTTGACCCAT
CAAGCGCAATGCTAGGCATTGATGCATTAGGCTACGAAGAAGAACAAAAAGCTTTATTTATGGAAGCTTTAGATAAACCA
CAAGGTATGCTTTTGATTACTGGACCAACAGGTTCCGGTAAAACTGTATCTCTATATACAGGCTTAAATATTTTAAATAC
TGAAAGTTCCAATATTTCTACTGCCGAAGATCCAGTGGAAATTAACTTAGAAGGTATTAATCAGGTTAACGTCAATCCTA
AAGTGGGTTTAACGTTTGCAGCTGCTCTTAAGTCTTTTTTACGTCAAGACCCTGACATTATTATGGTCGGTGAGATTCGT
GACTTGGAAACAGCAGAAATTGCGATTAAAGCTGCTCAAACCGGCCATATGGTGATGTCAACACTACATACCAATAGTGC
TCCGGAAACATTGACCCGTCTACGCAATATGGGGGTTCCTTCTTTTAATATTGCAACATCAGTAAATCTAGTGATTGCAC
AGCGTTTAGCACGTCGTCTTTGTTCTCAATGTAAAATACCTGCTGATATTCCTAAACAAAGCTTATTAGAAATGGGCTTT
ACCGAACAAGATTTAGCACATCCAGATTTTCGGGTTTTTCAACCCGTGGGCTGTCCTGAATGTCGTGAAGGCTATAAAGG
CCGTGTCGGTATTTATGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAACGCTTTAGAAA
TCGCGGCTGCTTCTGAAAAATTGGGGTTTAATAATCTACGTCGTTCAGGTTTAAAAAAGGTGATGCAAGGGGTGACCTCT
TTACAAGAAGTTAACCGTGTAACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K9BAJ6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

98.596

100

0.986
  pilB Acinetobacter baylyi ADP1

79.825

100

0.798
  pilB Legionella pneumophila strain ERS1305867

50.794

99.474

0.505
  pilF Neisseria gonorrhoeae MS11

50.178

98.596

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

51.698

92.982

0.481
  pilB Vibrio campbellii strain DS40M4

50.867

91.053

0.463
  pilB Vibrio cholerae strain A1552

50.29

90.702

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

35.179

100

0.379
  pilF Thermus thermophilus HB27

37.011

98.596

0.365

Multiple sequence alignment