Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FWK58_RS07850 Genome accession   NZ_CP042991
Coordinates   1742814..1744205 (-) Length   463 a.a.
NCBI ID   WP_136122165.1    Uniprot ID   -
Organism   Histophilus somni strain UOC-KLM-ATR-012     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1737814..1749205
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FWK58_RS07835 (FWK58_07835) ffh 1739128..1740504 (-) 1377 WP_075293626.1 signal recognition particle protein -
  FWK58_RS07840 (FWK58_07840) - 1740654..1741451 (+) 798 WP_075293627.1 inner membrane protein YpjD -
  FWK58_RS07845 (FWK58_07845) - 1741521..1742780 (+) 1260 WP_012341088.1 HlyC/CorC family transporter -
  FWK58_RS07850 (FWK58_07850) pilB 1742814..1744205 (-) 1392 WP_136122165.1 GspE/PulE family protein Machinery gene
  FWK58_RS07855 (FWK58_07855) tig 1744588..1745886 (+) 1299 WP_136122166.1 trigger factor -
  FWK58_RS07860 (FWK58_07860) clpP 1745972..1746553 (+) 582 WP_075293629.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP -
  FWK58_RS07865 (FWK58_07865) clpX 1746569..1747813 (+) 1245 WP_075293630.1 ATP-dependent protease ATP-binding subunit ClpX -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52927.78 Da        Isoelectric Point: 6.2978

>NTDB_id=379963 FWK58_RS07850 WP_136122165.1 1742814..1744205(-) (pilB) [Histophilus somni strain UOC-KLM-ATR-012]
MSPIVMSIDGDTYEITPHLWQRNQQQSQILLRYFAIPLQENEQTLWLGVDSLNNISACETFAFLYGKIVEPVLLNNQLLK
QLLQNLSPQQNNMFVEEQPINQYVAEHRIEVTEKSDEPVIQLLDHIFENALKQHVSDIHIEPQMNCLQIRFRIDGILQCQ
SPLPLSLSKRILSRLKLLAKLDISETRLPQDGRFHFKTTFSDILDFRLSTLPTNMGEKAVLRLQQNKPVQLSFAELGMTE
NQQKSFKQALSQPQGLILVTGPTGSGKSISLYTALQWLNDKHKHIMTAEDPIEIELNGIIQCQINPQIGLDFSRLLRTFL
RQDPDIIMLGEIRDNESAIMALRAAQTGHLVLSTLHTNDAPSAVSRLLQLGVKQHEIDNSLLLVIAQRLVRKKCPHTENE
NCTCHQKYQGRIGVYQFLQPHLIDNHICYQTDYAHLRESAMEKVRLEITDLAEVDRVIGQSSE

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=379963 FWK58_RS07850 WP_136122165.1 1742814..1744205(-) (pilB) [Histophilus somni strain UOC-KLM-ATR-012]
ATGTCTCCTATAGTGATGTCGATCGATGGAGATACTTATGAAATAACACCGCACTTATGGCAAAGAAATCAGCAACAATC
CCAAATTCTGCTACGCTATTTTGCCATTCCTTTACAGGAAAATGAACAAACGTTATGGTTAGGCGTTGATAGTCTAAATA
ATATTAGTGCCTGTGAAACATTTGCATTTTTGTATGGAAAAATTGTAGAACCGGTTTTACTTAATAACCAATTACTTAAA
CAGTTACTACAAAACTTATCACCACAGCAAAATAATATGTTCGTTGAAGAGCAACCTATAAATCAATATGTAGCCGAGCA
TCGTATAGAAGTAACTGAAAAGTCTGATGAACCCGTGATTCAACTTTTAGATCACATTTTTGAAAATGCGTTAAAGCAAC
ATGTATCAGATATTCATATTGAACCGCAAATGAATTGCTTGCAAATACGTTTCAGAATAGACGGTATTTTACAATGCCAA
TCGCCTCTTCCGCTTTCGCTGAGTAAGCGTATTCTTTCACGTCTAAAGTTACTGGCCAAATTGGATATAAGCGAAACACG
GTTACCTCAAGATGGACGATTTCATTTTAAAACAACATTTTCTGACATCCTTGATTTTCGTTTATCAACCCTTCCGACGA
ATATGGGTGAAAAAGCCGTGTTGCGTTTACAGCAAAATAAACCTGTACAGTTGAGTTTTGCGGAACTCGGCATGACAGAA
AACCAGCAAAAATCATTCAAACAGGCACTATCTCAACCCCAAGGACTCATTTTAGTGACCGGTCCAACAGGGAGTGGAAA
AAGTATCTCACTTTACACCGCACTTCAATGGCTGAATGATAAGCACAAACATATTATGACAGCGGAAGATCCGATAGAAA
TTGAATTGAACGGTATCATTCAATGTCAAATAAATCCGCAGATCGGGTTAGATTTTAGCCGACTGTTAAGAACTTTTCTT
CGTCAAGATCCTGACATTATTATGTTGGGTGAAATTCGGGATAATGAAAGTGCAATAATGGCATTAAGAGCAGCACAAAC
CGGACATCTTGTTCTCTCAACTTTACATACAAATGATGCTCCATCGGCAGTTTCACGCTTGCTTCAATTAGGGGTAAAGC
AACATGAAATTGACAATAGTTTATTATTGGTCATCGCTCAACGCTTGGTACGAAAAAAATGTCCACATACAGAAAATGAA
AATTGCACTTGTCATCAGAAATATCAAGGGCGAATTGGTGTTTATCAATTTTTACAACCGCATCTAATAGATAATCATAT
TTGCTACCAAACGGATTATGCTCATTTGCGTGAAAGTGCAATGGAAAAAGTGCGGTTAGAAATAACAGATTTAGCAGAAG
TGGATAGAGTTATTGGACAAAGTAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(126-392)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-90)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

68.33

99.568

0.68
  pilB Haemophilus influenzae Rd KW20

67.609

99.352

0.672
  pilB Glaesserella parasuis strain SC1401

57.333

97.192

0.557
  pilB Vibrio campbellii strain DS40M4

40.456

100

0.421
  pilB Vibrio cholerae strain A1552

39.212

100

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

38.969

100

0.408
  pilB Legionella pneumophila strain ERS1305867

37.045

100

0.395
  pilB Acinetobacter baylyi ADP1

36.128

100

0.391
  pilB Acinetobacter baumannii D1279779

37.474

100

0.391
  pilF Neisseria gonorrhoeae MS11

35.865

100

0.367

Multiple sequence alignment