Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FWK48_RS07975 Genome accession   NZ_CP042987
Coordinates   1742784..1744175 (-) Length   463 a.a.
NCBI ID   WP_136121732.1    Uniprot ID   -
Organism   Histophilus somni strain UOC-KLM-ATR-08     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1737784..1749175
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FWK48_RS07960 (FWK48_07960) ffh 1739102..1740478 (-) 1377 WP_132994679.1 signal recognition particle protein -
  FWK48_RS07965 (FWK48_07965) - 1740624..1741421 (+) 798 WP_136121711.1 inner membrane protein YpjD -
  FWK48_RS07970 (FWK48_07970) - 1741491..1742750 (+) 1260 WP_012341088.1 HlyC/CorC family transporter -
  FWK48_RS07975 (FWK48_07975) pilB 1742784..1744175 (-) 1392 WP_136121732.1 GspE/PulE family protein Machinery gene
  FWK48_RS07980 (FWK48_07980) tig 1744558..1745856 (+) 1299 WP_136121710.1 trigger factor -
  FWK48_RS07985 (FWK48_07985) clpP 1745942..1746523 (+) 582 WP_075293629.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP -
  FWK48_RS07990 (FWK48_07990) clpX 1746539..1747783 (+) 1245 WP_136121709.1 ATP-dependent protease ATP-binding subunit ClpX -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52931.77 Da        Isoelectric Point: 6.2293

>NTDB_id=379865 FWK48_RS07975 WP_136121732.1 1742784..1744175(-) (pilB) [Histophilus somni strain UOC-KLM-ATR-08]
MSPIVMSIDGDTYEITPHLWQRNQQQSQILLRYFAIPLQENEQTLWLGVDSLNNISACETFAFLYGKIVEPVLLNNQLLK
QLLQNLSPQQNNMFVEEQPINQYVAEHRIEVTEKSDEPVIQLLDHIFENALKQNVSDIHIEPQMNCLQIRFRIDGILQCQ
SPLPLSLSKRILSRLKLLAKLDISETRLPQDGRFHFKTTFSDILDFRLSTLPTNMGEKAVLRLQQNKPVQLSFAELGMTE
NQQKSFKQALSQPQGLILVTGPTGSGKSISLYTALQWLNDKHKHIMTAEDPIEIELNGIIQCQINPQIGLDFSRLLRTFL
RQDPDIIMLGEIRDNESAIMALRAAQTGHLVLSTLHTNDAPSAVSRLLQLGVKQHEIDNSLLLVIAQRLVRKKCPHTENE
NCTCHQKYQGRIGVYQFLQPHLIDNHICYQTDYAHLRESAMEKVRLEITDLAEVDRVIGQSNE

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=379865 FWK48_RS07975 WP_136121732.1 1742784..1744175(-) (pilB) [Histophilus somni strain UOC-KLM-ATR-08]
ATGTCTCCGATAGTGATGTCGATCGATGGAGATACTTATGAAATAACACCGCACTTATGGCAAAGAAATCAGCAACAATC
CCAAATTCTGCTACGCTATTTTGCCATTCCTTTACAGGAAAATGAACAAACGTTATGGTTAGGCGTTGATAGTCTAAATA
ATATTAGTGCCTGTGAAACATTTGCATTTTTGTATGGAAAAATTGTAGAACCGGTTTTACTTAATAACCAATTACTTAAA
CAGTTACTACAAAACTTATCACCACAGCAAAATAATATGTTCGTTGAAGAGCAACCTATAAATCAATATGTAGCCGAGCA
TCGTATAGAAGTAACTGAAAAGTCTGATGAACCCGTGATTCAACTTTTAGATCACATTTTTGAAAATGCGTTAAAGCAAA
ATGTATCAGATATTCATATTGAACCGCAAATGAATTGCTTGCAAATACGTTTCAGAATAGACGGTATTTTACAATGCCAA
TCGCCTCTTCCGCTTTCGCTGAGTAAGCGTATTCTTTCACGTTTAAAGTTACTGGCCAAATTGGATATAAGCGAAACACG
GTTACCTCAAGATGGACGATTTCATTTTAAAACAACATTTTCTGACATCCTTGATTTTCGTTTATCAACCCTTCCGACGA
ATATGGGTGAAAAAGCCGTGTTGCGTTTACAGCAAAATAAACCTGTACAGTTGAGTTTTGCGGAACTCGGCATGACAGAA
AACCAGCAAAAATCATTCAAACAGGCACTATCTCAACCCCAAGGACTCATTTTAGTGACAGGCCCAACAGGGAGTGGAAA
AAGTATCTCACTTTACACCGCACTTCAATGGCTGAATGATAAGCACAAACATATTATGACAGCGGAAGATCCGATAGAAA
TTGAATTGAACGGTATCATTCAATGTCAAATAAATCCGCAGATCGGGTTAGATTTTAGCCGGCTGTTAAGAACTTTTCTT
CGTCAAGATCCTGACATTATTATGTTGGGTGAAATTCGGGATAACGAAAGTGCAATAATGGCATTAAGAGCAGCACAAAC
CGGACATCTTGTTCTCTCAACTTTACATACAAATGATGCTCCGTCGGCAGTTTCACGCTTGCTTCAATTGGGGGTAAAGC
AACATGAAATTGACAATAGTTTATTATTGGTCATCGCTCAACGCTTGGTACGAAAAAAATGTCCACATACAGAAAATGAA
AATTGCACTTGTCATCAGAAATATCAAGGGCGAATTGGTGTTTATCAATTTTTACAACCGCATCTAATAGATAATCATAT
TTGCTACCAAACGGATTATGCTCATTTGCGTGAAAGTGCAATGGAAAAAGTGCGGTTAGAAATAACAGATTTAGCAGAAG
TGGATAGAGTTATTGGACAAAGTAATGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-90)

Type II/IV secretion system protein (T2SSE)

(126-392)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

68.33

99.568

0.68
  pilB Haemophilus influenzae Rd KW20

67.826

99.352

0.674
  pilB Glaesserella parasuis strain SC1401

57.556

97.192

0.559
  pilB Vibrio campbellii strain DS40M4

40.456

100

0.421
  pilB Vibrio cholerae strain A1552

39.212

100

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

38.969

100

0.408
  pilB Legionella pneumophila strain ERS1305867

37.045

100

0.395
  pilB Acinetobacter baylyi ADP1

36.128

100

0.391
  pilB Acinetobacter baumannii D1279779

37.474

100

0.391
  pilF Neisseria gonorrhoeae MS11

35.865

100

0.367

Multiple sequence alignment