Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FWK44_RS01860 Genome accession   NZ_CP042984
Coordinates   368782..370173 (+) Length   463 a.a.
NCBI ID   WP_101812545.1    Uniprot ID   A0AAX2RXF4
Organism   Histophilus somni strain UOC-KLM-ATR-03     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 363782..375173
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FWK44_RS01845 (FWK44_01845) clpX 365174..366418 (-) 1245 WP_075293630.1 ATP-dependent protease ATP-binding subunit ClpX -
  FWK44_RS01850 (FWK44_01850) clpP 366434..367015 (-) 582 WP_012341086.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP -
  FWK44_RS01855 (FWK44_01855) tig 367101..368399 (-) 1299 WP_011609585.1 trigger factor -
  FWK44_RS01860 (FWK44_01860) pilB 368782..370173 (+) 1392 WP_101812545.1 GspE/PulE family protein Machinery gene
  FWK44_RS01865 (FWK44_01865) - 370207..371466 (-) 1260 WP_132994681.1 HlyC/CorC family transporter -
  FWK44_RS01870 (FWK44_01870) - 371536..372333 (-) 798 WP_132994680.1 inner membrane protein YpjD -
  FWK44_RS01875 (FWK44_01875) ffh 372479..373855 (+) 1377 WP_132994679.1 signal recognition particle protein -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52954.80 Da        Isoelectric Point: 6.2978

>NTDB_id=379787 FWK44_RS01860 WP_101812545.1 368782..370173(+) (pilB) [Histophilus somni strain UOC-KLM-ATR-03]
MSPIVMSLDGDTYEITPHLWQRNQQQSQILLRYFAIPLQENEQTLWLGVDSLNNISACETFAFLYGKIVEPVLLNNQLLK
QLLQNLSPQQNNMFVEEQPINQYVAEHRIEVTEKSDEPVIQLLDHIFENALKQHVSDIHIEPQMNCLQIRFRIDGILQCQ
SPLPLSLSKRILSRLKLLAKLDISETRLPQDGRFHFKTTFSDILDFRLSTLPTNMGEKAVLRLQQNKPVQLSFAELGMTE
NQQKSFKQALSQPQGLILVTGPTGSGKSISLYTALQWLNDKHKHIMTAEDPIEIELNGIIQCQINPQIGLDFSRLLRTFL
RQDPDIIMLGEIRDNESAIMALRAAQTGHLVLSTLHTNDAPSAVSRLLQLGVKQHEIDNSLLLVIAQRLVRKKCPHTENE
NCTCHQKYQGRIGVYQFLQPHLIDNHICYQTDYAHLRESAMEKVRLEITDLAEVDRVIGQSNE

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=379787 FWK44_RS01860 WP_101812545.1 368782..370173(+) (pilB) [Histophilus somni strain UOC-KLM-ATR-03]
ATGTCTCCGATAGTGATGTCGCTCGATGGAGATACTTATGAAATAACACCGCACTTATGGCAAAGAAATCAGCAACAATC
CCAAATTCTGCTACGCTATTTTGCCATTCCTTTACAGGAAAATGAACAAACGTTATGGTTAGGCGTTGATAGTCTAAATA
ATATTAGTGCCTGTGAAACATTTGCATTTTTGTATGGAAAAATTGTAGAACCGGTTTTACTTAATAACCAATTACTTAAA
CAGTTACTACAAAACTTATCACCACAGCAAAATAATATGTTCGTTGAAGAGCAACCTATAAATCAATATGTAGCCGAGCA
TCGTATAGAAGTAACTGAAAAGTCTGATGAACCCGTGATTCAACTTTTAGATCACATTTTTGAAAATGCGTTAAAGCAAC
ATGTATCAGATATTCATATTGAACCGCAAATGAATTGCTTGCAAATACGTTTCAGAATAGACGGTATTTTACAATGCCAA
TCGCCTCTTCCGCTTTCGCTGAGTAAGCGTATTCTTTCACGTTTAAAGTTACTGGCCAAATTGGATATAAGCGAAACACG
GTTACCTCAAGATGGACGATTTCATTTTAAAACAACATTTTCTGACATCCTTGATTTTCGTTTATCAACCCTTCCGACGA
ATATGGGTGAAAAAGCCGTGTTGCGTTTACAGCAAAATAAACCTGTACAGTTGAGTTTTGCGGAACTCGGCATGACAGAA
AACCAGCAAAAATCATTCAAACAGGCACTATCTCAACCTCAAGGACTCATTTTAGTGACAGGCCCAACAGGGAGTGGAAA
AAGTATCTCACTTTACACCGCACTTCAATGGCTGAATGATAAGCACAAACATATTATGACAGCGGAAGATCCGATAGAAA
TTGAATTGAACGGTATCATTCAATGTCAAATAAATCCGCAGATCGGGTTAGATTTTAGCCGGCTGTTAAGAACTTTTCTT
CGTCAAGATCCTGACATTATTATGTTGGGTGAAATTCGGGATAACGAAAGTGCAATAATGGCATTAAGAGCAGCACAAAC
TGGACATCTTGTTCTCTCAACTTTACATACAAATGATGCTCCGTCGGCAGTCTCACGCTTGCTTCAATTAGGGGTAAAGC
AACATGAAATTGACAATAGTTTATTATTGGTCATCGCTCAACGTTTGGTACGAAAAAAATGCCCACATACAGAAAATGAA
AATTGCACTTGTCATCAGAAATATCAAGGGCGAATTGGTGTTTATCAATTTTTACAACCGCATCTAATAGATAATCATAT
TTGCTACCAAACGGATTATGCTCATTTGCGTGAAAGTGCAATGGAAAAAGTGCGGTTAGAAATAACAGATTTAGCAGAAG
TGGATAGAGTTATTGGACAAAGTAATGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-90)

Type II/IV secretion system protein (T2SSE)

(126-392)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

68.113

99.568

0.678
  pilB Haemophilus influenzae Rd KW20

67.609

99.352

0.672
  pilB Glaesserella parasuis strain SC1401

57.333

97.192

0.557
  pilB Vibrio campbellii strain DS40M4

40.456

100

0.421
  pilB Vibrio cholerae strain A1552

39.212

100

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

38.969

100

0.408
  pilB Legionella pneumophila strain ERS1305867

37.045

100

0.395
  pilB Acinetobacter baylyi ADP1

36.128

100

0.391
  pilB Acinetobacter baumannii D1279779

37.474

100

0.391
  pilF Neisseria gonorrhoeae MS11

35.865

100

0.367

Multiple sequence alignment