Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HBK86_RS01315 Genome accession   NZ_CP050385
Coordinates   262240..263730 (+) Length   496 a.a.
NCBI ID   WP_001166326.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain VB82     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 257240..268730
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HBK86_RS01300 (HBK86_01300) - 257598..260405 (+) 2808 WP_000276853.1 DUF2339 domain-containing protein -
  HBK86_RS01305 (HBK86_01305) - 260395..261312 (-) 918 WP_000785635.1 mechanosensitive ion channel family protein -
  HBK86_RS01310 (HBK86_01310) - 261322..262143 (-) 822 WP_000462733.1 hypothetical protein -
  HBK86_RS01315 (HBK86_01315) pilB 262240..263730 (+) 1491 WP_001166326.1 type II secretion system ATPase GspE Machinery gene
  HBK86_RS01320 (HBK86_01320) - 263785..264216 (-) 432 WP_000051124.1 organic hydroperoxide resistance protein -
  HBK86_RS01325 (HBK86_01325) - 264304..264741 (-) 438 WP_000371731.1 MarR family winged helix-turn-helix transcriptional regulator -
  HBK86_RS01330 (HBK86_01330) - 264901..265278 (-) 378 WP_000266123.1 DUF4951 domain-containing protein -
  HBK86_RS01335 (HBK86_01335) - 265293..266117 (-) 825 WP_000177091.1 carbon-nitrogen hydrolase family protein -
  HBK86_RS01340 (HBK86_01340) gigB 266144..266665 (-) 522 WP_000103065.1 anti-anti-sigma factor GigB -
  HBK86_RS01345 (HBK86_01345) gigA 266768..267736 (-) 969 WP_000273589.1 RsbU family protein phosphatase GigA -

Sequence

Protein


Download         Length: 496 a.a.        Molecular weight: 55329.22 Da        Isoelectric Point: 6.2037

>NTDB_id=379529 HBK86_RS01315 WP_001166326.1 262240..263730(+) (pilB) [Acinetobacter baumannii strain VB82]
MQILKQIQVPYSFAKRHGVLFRYDGDQVFIVRRQNTEKVALQEARRILGKPAHYQLCTEQEFNSLLSTSYAGDTGESQQV
AAGLEDHPDLLSLADQVPETEDLMDQEDDAPIVRLINALLSEAIRVGASDIHIEAFEKKLSVRLRVDGQLREIVQPRREL
APLLVSRIKVMAKLDIAEKRIPQDGRISLRLAGREVDVRVSTLPSSHGERVVMRLLDKQAGRLNMTHLGLMANDYERLTQ
LVHRPHGIILVTGPTGSGKTTTLYAALSDLNDNTRNILTAEDPIEYQLEGIGQTQVNTKVDMTFARALKAMLRQDPDVVM
VGEIRDLETAEIAVQASLTGHLVLSTLHTNTAIGAVTRLKDMGIEPFLLSSSLIGVVAQRLVRTLCPHCMTWREADTFEK
QVFQHISHEPSLKLPEAQGCDQCSHLGFNGRTAIYEIVPIDEPMRRLIHGNAAEFELENHARQYSGSIRDDGLRKVLSGK
TTLEEVLRVTNEASEA

Nucleotide


Download         Length: 1491 bp        

>NTDB_id=379529 HBK86_RS01315 WP_001166326.1 262240..263730(+) (pilB) [Acinetobacter baumannii strain VB82]
ATGCAAATATTGAAACAAATACAAGTTCCATATAGTTTTGCCAAGCGTCATGGTGTTTTATTTCGTTATGACGGTGATCA
AGTTTTTATTGTTAGACGTCAAAATACAGAAAAAGTTGCATTGCAGGAAGCAAGACGAATTTTGGGGAAACCTGCACACT
ATCAATTATGCACTGAACAAGAATTCAATAGCTTATTAAGTACCAGTTATGCAGGTGATACAGGTGAATCTCAACAAGTA
GCTGCGGGCTTAGAAGACCATCCAGATTTATTAAGCCTTGCAGATCAGGTGCCTGAAACAGAAGACTTAATGGATCAAGA
GGATGACGCGCCTATTGTTCGTCTAATCAATGCTCTTCTTTCTGAGGCAATTCGTGTTGGAGCTTCAGATATCCATATTG
AAGCCTTTGAGAAAAAGCTATCAGTTCGTTTACGGGTAGATGGTCAATTACGTGAAATTGTGCAGCCACGCCGTGAACTG
GCCCCGTTACTTGTTTCGCGTATTAAAGTTATGGCGAAACTCGATATTGCCGAAAAGCGTATACCGCAAGATGGACGTAT
TTCTTTACGTCTTGCTGGTCGTGAAGTCGATGTCCGTGTTTCAACTTTGCCATCGTCACATGGTGAAAGGGTGGTCATGC
GTTTACTCGATAAACAAGCGGGTCGCCTTAATATGACTCACCTAGGTTTAATGGCAAATGACTATGAACGTTTAACTCAG
CTAGTACATCGTCCGCACGGTATTATTTTGGTCACAGGGCCTACTGGTTCGGGGAAAACGACGACTTTATATGCTGCCTT
ATCTGATCTAAATGACAATACCCGTAACATCTTAACTGCCGAAGATCCAATTGAGTATCAGCTTGAAGGTATTGGCCAAA
CTCAAGTGAATACCAAAGTGGATATGACTTTTGCTCGAGCTTTAAAGGCAATGTTACGTCAAGATCCAGATGTGGTGATG
GTGGGGGAGATTCGTGATCTGGAAACTGCTGAAATTGCAGTTCAAGCATCTTTAACAGGTCATTTGGTTTTATCGACGCT
ACATACCAACACTGCTATTGGTGCAGTGACTCGACTTAAAGATATGGGGATTGAACCTTTCCTGCTTTCAAGCTCTTTAA
TTGGGGTGGTTGCGCAGCGTTTGGTTCGTACTTTATGTCCACATTGTATGACATGGCGTGAAGCCGATACTTTTGAGAAG
CAAGTTTTTCAGCATATTTCCCATGAACCAAGTTTAAAGTTACCAGAGGCTCAAGGGTGTGATCAGTGTTCTCATTTAGG
GTTTAATGGCCGTACAGCGATTTATGAAATTGTACCTATTGATGAACCTATGCGCCGTCTCATTCATGGTAATGCAGCTG
AATTTGAATTAGAAAATCATGCTCGTCAGTATTCTGGTTCTATTCGCGATGATGGTTTACGTAAGGTGCTCTCAGGTAAA
ACTACACTTGAAGAAGTGCTTCGTGTAACCAATGAAGCAAGTGAAGCATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(118-384)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

41.803

98.387

0.411
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.854

99.194

0.405
  pilF Thermus thermophilus HB27

39.351

99.395

0.391
  pilB Vibrio campbellii strain DS40M4

48.371

80.444

0.389
  pilB/pilB1 Synechocystis sp. PCC 6803

40.041

97.177

0.389
  pilF Neisseria gonorrhoeae MS11

48.731

79.435

0.387
  pilB Vibrio parahaemolyticus RIMD 2210633

48

80.645

0.387
  pilB Vibrio cholerae strain A1552

48

80.645

0.387
  pilB Acinetobacter baumannii D1279779

46.898

81.25

0.381
  pilB Acinetobacter baylyi ADP1

46.53

78.427

0.365

Multiple sequence alignment