Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RPSI07_RS21230 Genome accession   NC_014311
Coordinates   2927645..2928166 (-) Length   173 a.a.
NCBI ID   WP_013213428.1    Uniprot ID   -
Organism   Ralstonia solanacearum PSI07     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2922645..2933166
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RPSI07_RS21210 (RPSI07_2794) moaC 2923302..2923778 (+) 477 WP_013213424.1 cyclic pyranopterin monophosphate synthase MoaC -
  RPSI07_RS21215 (RPSI07_2795) - 2924223..2925959 (-) 1737 WP_013213425.1 PglL family O-oligosaccharyltransferase -
  RPSI07_RS21220 (RPSI07_2796) pilA 2926040..2926543 (-) 504 WP_013213426.1 pilin Machinery gene
  RPSI07_RS26430 (RPSI07_2797) - 2926912..2927208 (+) 297 WP_231890111.1 hypothetical protein -
  RPSI07_RS21225 (RPSI07_2798) - 2927172..2927591 (+) 420 WP_231890112.1 IS1595 family transposase -
  RPSI07_RS21230 (RPSI07_2799) pilA 2927645..2928166 (-) 522 WP_013213428.1 pilin Machinery gene
  RPSI07_RS21235 (RPSI07_2800) - 2928360..2929058 (-) 699 WP_013213429.1 TerC family protein -
  RPSI07_RS21240 (RPSI07_2801) sucD 2929213..2930094 (-) 882 WP_013213430.1 succinate--CoA ligase subunit alpha -
  RPSI07_RS21245 (RPSI07_2802) sucC 2930179..2931345 (-) 1167 WP_013213431.1 ADP-forming succinate--CoA ligase subunit beta -
  RPSI07_RS21250 (RPSI07_2803) - 2931413..2932039 (-) 627 WP_013213432.1 DUF2889 domain-containing protein -
  RPSI07_RS21255 (RPSI07_2804) recX 2932312..2932791 (-) 480 WP_013213433.1 recombination regulator RecX -

Sequence

Protein


Download         Length: 173 a.a.        Molecular weight: 17677.29 Da        Isoelectric Point: 10.0311

>NTDB_id=37745 RPSI07_RS21230 WP_013213428.1 2927645..2928166(-) (pilA) [Ralstonia solanacearum PSI07]
MASKHSRNRRRGQRGFTLIELMIVVAIIGILAAIAIPAYQDYTIRARVTEGLSLAAQAKALVVENAANAQSSLALGSASL
PASRNVSALDIDSSTGEVTVVYASAVAGTGANTLVLTPYSGSSASVTNLQAGSAPPGMVLWVCAAAGKSMPVNTVTQKTT
ATLPSKYAPAECR

Nucleotide


Download         Length: 522 bp        

>NTDB_id=37745 RPSI07_RS21230 WP_013213428.1 2927645..2928166(-) (pilA) [Ralstonia solanacearum PSI07]
ATGGCATCGAAGCACAGCAGGAACCGGCGGCGCGGCCAGCGCGGCTTTACATTGATTGAGTTGATGATCGTGGTGGCGAT
CATTGGGATATTGGCGGCGATTGCGATTCCGGCGTACCAGGACTACACGATCCGCGCGCGCGTGACCGAAGGTCTGTCGC
TGGCTGCGCAGGCCAAGGCGCTGGTGGTTGAGAACGCGGCCAACGCGCAGAGTAGCTTGGCGCTTGGCTCCGCATCCCTG
CCGGCGAGCAGGAATGTCAGTGCTTTGGATATTGATTCGTCGACGGGCGAGGTTACTGTGGTCTACGCGTCTGCCGTTGC
CGGTACGGGCGCGAATACGCTGGTATTGACCCCGTACTCCGGAAGCTCGGCATCGGTTACGAACTTGCAGGCGGGTTCAG
CGCCGCCGGGAATGGTGCTGTGGGTGTGCGCGGCCGCTGGCAAGTCGATGCCTGTGAACACAGTGACCCAGAAAACAACG
GCCACATTGCCATCGAAGTATGCGCCTGCAGAGTGTCGCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-35)

Pilin (bacterial filament) (Pilin)

(45-173)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

55.682

100

0.566
  pilA2 Legionella pneumophila str. Paris

47.879

95.376

0.457
  pilA2 Legionella pneumophila strain ERS1305867

47.273

95.376

0.451
  comP Acinetobacter baylyi ADP1

42.073

94.798

0.399
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

35.789

100

0.393

Multiple sequence alignment