Detailed information NTG0037690

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AOLE_RS15205
• Genome accession: NC_014259
• Coordinates: 3244135..3245253 (+)
• Length: 372 a.a.
• NCBI ID: WP_013198760.1
• Uniprot ID: A0AAN0UEB6
• Organism: Acinetobacter oleivorans DR1
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3239135..3250253

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AOLE_RS15190 (AOLE_15215)	-	3241309..3242151 (-)	843	WP_013198758.1	undecaprenyl-diphosphate phosphatase	-
AOLE_RS15195 (AOLE_15220)	-	3242259..3242951 (-)	693	WP_013198759.1	YggS family pyridoxal phosphate-dependent enzyme	-
AOLE_RS15200 (AOLE_15225)	pilT	3243078..3244115 (+)	1038	WP_003653239.1	type IV pilus twitching motility protein PilT	Machinery gene
AOLE_RS15205 (AOLE_15230)	pilU	3244135..3245253 (+)	1119	WP_013198760.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AOLE_RS15210 (AOLE_15235)	fur	3245323..3245760 (-)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
AOLE_RS15215 (AOLE_15240)	-	3245872..3246270 (+)	399	WP_003653245.1	outer membrane protein assembly factor BamE	-
AOLE_RS15220 (AOLE_15245)	-	3246335..3246649 (-)	315	WP_013198761.1	RnfH family protein	-
AOLE_RS15225 (AOLE_15250)	-	3246649..3247716 (-)	1068	WP_023274309.1	hypothetical protein	-
AOLE_RS15230 (AOLE_15255)	-	3247868..3248323 (-)	456	WP_013198763.1	bacteriohemerythrin	-
AOLE_RS15235 (AOLE_15260)	-	3248573..3249421 (-)	849	WP_013198764.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41887.10 Da
• Isoelectric Point: 6.6401

>NTDB_id=37690 AOLE_RS15205 WP_013198760.1 3244135..3245253(+) (pilU) [Acinetobacter oleivorans DR1]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNTKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLAEEGPDQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=37690 AOLE_RS15205 WP_013198760.1 3244135..3245253(+) (pilU) [Acinetobacter oleivorans DR1]
ATGGATTTTAATGACTTACTCAACCTCATGGTTGAAAAAAAATCATCCGATCTTTTTATTACAGATGGTGTTGCGCCATC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCAGGCGAAGTAATTGGTCAACTCTTACATTCCA
TCATGAGCGAAAAGCAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATCATGAACCGTGATAAAACTGCC
CGCTTTCGTGTGAGTGCTTTTCAGCAGCGTGACATGCCCGGCATGGTGCTACGTCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTGCAGTTACCACCTGTTCTTAAAGATTTATCAATGACCAAACGCGGTATCATTATTTTTGTTGGTGCGACAG
GTACAGGTAAATCGACGTCTTTGGCTTCAATGATTAGCTATCGTAACCACAACACTAAAGGTCATATCATTACCATTGAA
GACCCAATCGAGTTTATTCACGAACATGCGGGCTGCATCATTACCCAACGTGAAGTCGGAATCGATACGGACTCATTTGA
AATTGCCTTAAAAAATACTTTGCGACAAGCACCAGATGTCATCTTGATTGGTGAGATTCGATCTCGTGAAGTTATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCCACAATGCACGCCAACAACGCTAACCAAGCACTCGACCGT
ATCATTCACTTCTTTGAAAGTGACCGCCACAGTCAGCTCTACATGGACTTATCTCTTAACTTAAAGGCCATGATTGCTCA
GCAACTTATTCCAACACCGGATGGCAATTCTCGCCGCGCCGCAATTGAAATTTTAATTAACTCACCATTAATTTCAGATT
ACATTCGTAAAGGTGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACCTTTGACCAA
GCGTTGTTTGATCTCTATAAAGCAGGCCAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAATGATTTACG
TTTGACGATTAAGCTTGCTGAAGAAGGACCTGATCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACCGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	74.868	100	0.761
pilU	Pseudomonas stutzeri DSM 10701	67.925	99.731	0.677
pilU	Vibrio cholerae strain A1552	54.054	99.462	0.538
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.659	97.849	0.398
pilT	Legionella pneumophila strain Lp02	39.645	90.86	0.36
pilT	Legionella pneumophila strain ERS1305867	39.645	90.86	0.36