Detailed information NTG0374981

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: FPL13_RS00715
• Genome accession: NZ_CP041968
• Coordinates: 131150..131587 (+)
• Length: 145 a.a.
• NCBI ID: WP_049513212.1
• Uniprot ID: A0A4U9ZRD8
• Organism: Streptococcus sp. KS 6
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 126150..136587

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FPL13_RS00680 (FPL13_00700)	glnA	126326..127672 (+)	1347	WP_049513221.1	type I glutamate--ammonia ligase	-
FPL13_RS00685 (FPL13_00705)	-	127860..128225 (+)	366	WP_049507022.1	DUF1033 family protein	-
FPL13_RS00690 (FPL13_00710)	comYA	128295..129236 (+)	942	WP_049513218.1	competence type IV pilus ATPase ComGA	Machinery gene
FPL13_RS00695 (FPL13_00715)	comGB	129178..130199 (+)	1022	Protein_137	competence type IV pilus assembly protein ComGB	-
FPL13_RS00700 (FPL13_00720)	comYC	130196..130513 (+)	318	WP_003028473.1	competence type IV pilus major pilin ComGC	Machinery gene
FPL13_RS00705 (FPL13_00725)	comYD	130473..130901 (+)	429	WP_049507639.1	competence type IV pilus minor pilin ComGD	Machinery gene
FPL13_RS00710 (FPL13_00730)	comGE/cglE	130873..131166 (+)	294	WP_049513214.1	competence type IV pilus minor pilin ComGE	Machinery gene
FPL13_RS00715 (FPL13_00735)	comGF/cglF	131150..131587 (+)	438	WP_049513212.1	competence type IV pilus minor pilin ComGF	Machinery gene
FPL13_RS00720 (FPL13_00740)	comGG	131568..131894 (+)	327	WP_264340993.1	competence type IV pilus minor pilin ComGG	-
FPL13_RS00725 (FPL13_00745)	-	131966..132918 (+)	953	Protein_143	class I SAM-dependent methyltransferase	-
FPL13_RS00730 (FPL13_00750)	-	132965..134164 (+)	1200	WP_049513208.1	acetate kinase	-
FPL13_RS00735 (FPL13_00755)	glmS	134557..136371 (+)	1815	WP_049513230.1	glutamine--fructose-6-phosphate transaminase (isomerizing)	-

Sequence

Protein

• Length: 145 a.a.
• Molecular weight: 16829.39 Da
• Isoelectric Point: 10.0941

>NTDB_id=374981 FPL13_RS00715 WP_049513212.1 131150..131587(+) (comGF/cglF) [Streptococcus sp. KS 6]
MFKANKVKAFTLLEALIALFVLSGSVLLFQSMTRLLAFEVQARQHSEQREWLLFADQLEAELARSSFEKVENNRLYMKQD
GKVIAFGKSSGQDFRKTNANGKGYQPMVYDVKKAEISQKNELIHVRLTFRRGLEREFVYRVETES

Nucleotide

• Length: 438 bp

>NTDB_id=374981 FPL13_RS00715 WP_049513212.1 131150..131587(+) (comGF/cglF) [Streptococcus sp. KS 6]
GTGTTCAAGGCAAATAAAGTGAAAGCTTTTACCCTTTTAGAGGCTCTGATTGCCTTATTTGTACTCAGCGGAAGTGTGCT
GCTTTTTCAGTCAATGACGCGTCTTCTGGCTTTTGAAGTTCAAGCCCGGCAACATAGTGAACAACGAGAATGGCTTTTGT
TTGCTGATCAACTAGAAGCAGAGTTGGCTCGTTCTTCATTTGAAAAGGTGGAGAATAATCGTTTATATATGAAACAGGAC
GGCAAGGTGATTGCATTTGGAAAGTCAAGTGGACAGGATTTCAGAAAAACCAATGCGAATGGGAAAGGTTACCAGCCGAT
GGTTTATGATGTGAAAAAAGCAGAAATCTCACAGAAAAATGAGCTGATTCATGTCAGACTGACGTTTAGGCGAGGATTGG
AAAGGGAGTTTGTCTATCGTGTGGAAACAGAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (39-134)

3D structure

• AlphaFold DB: A0A4U9ZRD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae R6	65.493	97.931	0.641
comGF/cglF	Streptococcus pneumoniae TIGR4	65.493	97.931	0.641
comGF/cglF	Streptococcus pneumoniae Rx1	65.493	97.931	0.641
comGF/cglF	Streptococcus pneumoniae D39	65.493	97.931	0.641
comGF/cglF	Streptococcus mitis NCTC 12261	65.714	96.552	0.634
comGF/cglF	Streptococcus mitis SK321	65.714	96.552	0.634
comYF	Streptococcus mutans UA159	43.704	93.103	0.407
comYF	Streptococcus mutans UA140	43.704	93.103	0.407
comGF	Lactococcus lactis subsp. cremoris KW2	41.481	93.103	0.386