Detailed information NTG0374310

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: FOV14_RS12285
• Genome accession: NZ_CP041784
• Coordinates: 2555436..2556050 (-)
• Length: 204 a.a.
• NCBI ID: WP_043867297.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain IM1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2550436..2561050

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FOV14_RS12255 (FOV14_12330)	rpsT	2550736..2551002 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
FOV14_RS12260 (FOV14_12335)	holA	2551019..2552060 (-)	1042	Protein_2369	DNA polymerase III subunit delta	-
FOV14_RS12265 (FOV14_12340)	-	2552100..2552254 (-)	155	Protein_2370	hypothetical protein	-
FOV14_RS12270 (FOV14_12345)	-	2552295..2552429 (+)	135	WP_003152870.1	YqzM family protein	-
FOV14_RS12275 (FOV14_12350)	comEC	2552448..2554799 (-)	2352	WP_234949402.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
FOV14_RS12280 (FOV14_12355)	-	2554800..2555369 (-)	570	WP_003152868.1	ComE operon protein 2	-
FOV14_RS12285 (FOV14_12360)	comEA	2555436..2556050 (-)	615	WP_043867297.1	helix-hairpin-helix domain-containing protein	Machinery gene
FOV14_RS12290 (FOV14_12365)	comER	2556109..2556930 (+)	822	WP_014305449.1	late competence protein ComER	-
FOV14_RS12295 (FOV14_12370)	-	2556999..2557736 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
FOV14_RS12300 (FOV14_12375)	rsfS	2557733..2558089 (-)	357	WP_003152864.1	ribosome silencing factor	-
FOV14_RS12305 (FOV14_12380)	yqeK	2558086..2558667 (-)	582	WP_003152863.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
FOV14_RS12310 (FOV14_12385)	-	2558657..2559226 (-)	570	WP_160249251.1	nicotinate-nucleotide adenylyltransferase	-
FOV14_RS12315 (FOV14_12390)	yhbY	2559237..2559527 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
FOV14_RS12320 (FOV14_12395)	aroE	2559521..2560363 (-)	843	WP_160249250.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22178.37 Da
• Isoelectric Point: 6.9685

>NTDB_id=374310 FOV14_RS12285 WP_043867297.1 2555436..2556050(-) (comEA) [Bacillus velezensis strain IM1]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEGNHPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=374310 FOV14_RS12285 WP_043867297.1 2555436..2556050(-) (comEA) [Bacillus velezensis strain IM1]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGGGAATCATCCGAAAACAG
CAGTTGGTGAAAAGGAAAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCAATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363