Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   FFH21_RS01815 Genome accession   NZ_CP041754
Coordinates   364646..366751 (+) Length   701 a.a.
NCBI ID   WP_011167465.1    Uniprot ID   Q48PH2
Organism   Pseudomonas sp. KBS0707     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 359646..371751
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FFH21_RS01795 (FFH21_001795) pilM 361791..362855 (+) 1065 WP_002551652.1 pilus assembly protein PilM Machinery gene
  FFH21_RS01800 (FFH21_001800) - 362855..363454 (+) 600 WP_004654254.1 PilN domain-containing protein -
  FFH21_RS01805 (FFH21_001805) pilO 363451..364074 (+) 624 WP_002551654.1 type 4a pilus biogenesis protein PilO -
  FFH21_RS01810 (FFH21_001810) pilP 364071..364598 (+) 528 WP_002551655.1 type 4a pilus biogenesis lipoprotein PilP -
  FFH21_RS01815 (FFH21_001815) pilQ 364646..366751 (+) 2106 WP_011167465.1 type IV pilus secretin PilQ Machinery gene
  FFH21_RS01820 (FFH21_001820) aroK 366756..367274 (+) 519 WP_002551657.1 shikimate kinase AroK -
  FFH21_RS01825 (FFH21_001825) aroB 367390..368493 (+) 1104 WP_002551658.1 3-dehydroquinate synthase -
  FFH21_RS01830 (FFH21_001830) - 368504..370123 (+) 1620 WP_004667264.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 75495.92 Da        Isoelectric Point: 5.0976

>NTDB_id=373749 FFH21_RS01815 WP_011167465.1 364646..366751(+) (pilQ) [Pseudomonas sp. KBS0707]
MTRILSIIGVSLCIAMLSPVLQAANLKTLDVAALPGDRIELKLAFDSPVPAPRGYTTAQPARIALDLPGVTSQLATKSRE
LGNGNARSVVVVQAQDRTRVIINLTMLSPYSSRVDGNNLYVVIGQGAAAAQASQPTTAMGAQRVAVPPPTPASARPFIPA
GVKAIRNVDFQRGELGEGNVIIDLSNPGIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFATPVQFVSASATGDKASI
LIEPSGAFDYSAFQTENKLTISVRPLTNEDLDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGSVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKTDERGSITVDDRTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVDYNKQLGVRWGGSTNTSGNG
KWTTYGLDNNGDEAGNTGSNVTSNVPFVDMGASGATSGIGLGFVTNNTLLDLELSAMEKTGNGEIVSQPKVVTSDKETAK
ILKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGET
IVIGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=373749 FFH21_RS01815 WP_011167465.1 364646..366751(+) (pilQ) [Pseudomonas sp. KBS0707]
ATGACCCGGATTCTCTCGATTATCGGCGTGTCGTTATGCATCGCGATGCTTTCGCCGGTTCTCCAGGCTGCCAACCTCAA
GACCCTGGATGTCGCCGCCCTGCCGGGTGATCGCATCGAGCTCAAGCTGGCATTCGACTCGCCGGTGCCTGCGCCGCGCG
GTTACACCACGGCGCAACCTGCGCGTATTGCGCTGGACCTGCCGGGCGTGACCAGTCAGTTGGCGACCAAAAGCCGTGAG
CTCGGTAACGGTAACGCACGCAGCGTGGTCGTTGTACAGGCTCAGGACCGCACGCGGGTAATCATCAACCTGACCATGCT
TTCGCCCTACAGCTCCCGCGTCGACGGCAACAACCTTTACGTGGTTATCGGTCAGGGCGCAGCCGCTGCGCAGGCTTCCC
AGCCAACCACCGCGATGGGCGCGCAACGGGTAGCGGTGCCGCCACCCACTCCGGCATCTGCCAGACCGTTTATCCCTGCG
GGCGTCAAGGCTATCAGGAATGTCGATTTCCAGCGCGGCGAGCTGGGTGAAGGCAATGTGATCATAGACCTGAGCAACCC
CGGTATCGCCCCGGATATTCAGGAGCAGGGCGGCAAGATCCGTGTCGACTTCGCCAAGACCCAACTGCCCGAACCGCTGC
GCGTGCGGCTGGACGTCAAGGACTTCGCCACGCCGGTGCAGTTTGTCAGTGCGTCGGCTACGGGTGACAAGGCCAGTATT
CTGATCGAACCGTCGGGTGCCTTCGACTACTCGGCGTTTCAGACCGAAAACAAGCTGACCATCAGTGTGCGGCCGTTGAC
CAATGAGGATCTTGACCGCCGCGCCGCTGACAAACCGGTGTACACCGGTGAAAAGCTATCGCTGAACTTCCAGGACATCG
ATGTACGCTCGGTGCTGCAACTGATTGCCGACTTCACCAACCTCAATCTGGTGGCCAGCGACACGGTTCAGGGCGGTATT
ACTCTGCGTCTGCAGAACGTGCCATGGGATCAGGCGCTGGACCTGGTGCTGAAAACCAAGGGCCTGGACAAGCGCAAGGT
CGGCAGCGTTCTGCTGGTCGCGCCCGCCGATGAAATTGCTGCCCGCGAGCGTCAGGAGCTTGAATCGCTCAAGCAGATCG
CCGAGCTGGCGCCCCTGCGTCGTGAATTGTTGCAGGTTAATTACGCCAAGGCTGCCGATATCGCCAAGCTGTTCCAGTCG
GTGACCAGCGCCGAGTCCAAGACCGACGAGCGTGGCTCGATCACTGTGGACGACCGGACCAACAACATCATTGCCTATCA
AACCCAGGACCGGCTCGACGAACTGCGTCGCATCGTCTCGCAACTGGACATTCCGGTGCGTCAGGTGATGATCGAGGCGC
GCATCGTCGAGGCCAATGTCGATTACAACAAGCAACTGGGTGTGCGCTGGGGCGGCTCGACCAACACCAGCGGCAATGGA
AAATGGACAACCTATGGCCTTGATAATAATGGCGATGAGGCAGGTAACACTGGCAGTAACGTGACCTCCAACGTGCCGTT
TGTCGATATGGGCGCATCTGGCGCAACCTCCGGCATTGGCCTGGGCTTCGTCACCAACAATACGTTGCTGGACCTTGAAC
TGAGCGCGATGGAGAAGACCGGCAACGGTGAAATCGTCTCCCAGCCCAAGGTAGTCACTTCCGACAAGGAAACCGCCAAG
ATCCTCAAGGGCACCGAGATTCCGTATCAGGAATCCAGCTCCAGCGGTGCAACCACGGTGAGCTTCAAGGAGGCCTCGCT
GTCGCTTGAGGTAACGCCACAGATCACGCCGGATAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACT
ACCTGAATGCGGTGCTTGGCGTACCACCGATCAAGAAAAACGAGGTCAACGCCAAAGTGCTGATTTCCGATGGCGAAACC
ATCGTCATTGGTGGTGTGTTCTCAAATACGCAAAGTAAAGTTGTCGATAAAGTGCCATTTCTTGGCGATGTACCGTATCT
TGGCCGGCTTTTCAGGCGCGATGTGGTATCCGAGTCCAAATCCGAGCTGTTGGTATTTTTGACTCCGCGTATCATGAACA
ACCAGGCGATTTCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(536-692)

AMIN domain (AMIN)

(38-121)

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)

Secretin and TonB N terminus short domain (STN)

(309-355)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q48PH2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.061

100

0.743
  comQ Acinetobacter baylyi ADP1

41.727

99.144

0.414
  pilQ Acinetobacter baumannii strain A118

37.926

100

0.397
  pilQ Acinetobacter baumannii D1279779

37.926

100

0.397
  pilQ Legionella pneumophila strain Lp02

38.696

98.431

0.381
  pilQ Legionella pneumophila strain ERS1305867

38.607

98.288

0.379

Multiple sequence alignment