Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   FFH90_RS03045 Genome accession   NZ_CP041753
Coordinates   663552..665627 (+) Length   691 a.a.
NCBI ID   WP_064615925.1    Uniprot ID   A0A2W7LEI8
Organism   Pseudomonas sp. ATCC 43928     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 658552..670627
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FFH90_RS03025 (FFH90_003025) comM 660764..661828 (+) 1065 WP_018928618.1 pilus assembly protein PilM Machinery gene
  FFH90_RS03030 (FFH90_003030) - 661828..662394 (+) 567 WP_138866666.1 PilN domain-containing protein -
  FFH90_RS03035 (FFH90_003035) pilO 662391..663014 (+) 624 WP_018928616.1 type 4a pilus biogenesis protein PilO -
  FFH90_RS03040 (FFH90_003040) - 663011..663538 (+) 528 WP_138866667.1 pilus assembly protein PilP -
  FFH90_RS03045 (FFH90_003045) pilQ 663552..665627 (+) 2076 WP_064615925.1 type IV pilus secretin PilQ Machinery gene
  FFH90_RS03050 (FFH90_003050) aroK 665632..666150 (+) 519 WP_019581860.1 shikimate kinase AroK -
  FFH90_RS03055 (FFH90_003055) aroB 666301..667401 (+) 1101 WP_138866668.1 3-dehydroquinate synthase -
  FFH90_RS03060 (FFH90_003060) - 667412..669025 (+) 1614 WP_064615929.1 AAA family ATPase -

Sequence

Protein


Download         Length: 691 a.a.        Molecular weight: 74745.92 Da        Isoelectric Point: 5.7805

>NTDB_id=373722 FFH90_RS03045 WP_064615925.1 663552..665627(+) (pilQ) [Pseudomonas sp. ATCC 43928]
MNRIFSTFGISLWIAFLSPLVQAANLKALDVAALPGDRVELKLSFDGPPPQPHGYTTEQPARIALDLPGVTSQLANKSRD
LGGGNARSATVVEAKDRTRLIIGLTQLTPYSARVEGNNLFVVVGQAAGNKASKPIASAPRAATAVPAPARASAPVGKAIR
GVDFQRGTQGEGNVVIDLSDPSIAPDIQERDGKIIVGFTKTQLPERLRVRLDVKDFATPVQFVNASATGDRAIISIEPSG
AFDYSTYQTDNKLTISIRPMTVDDLQKRNAERFAYSGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KVDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQKGNWNGSGVNG
SSTTVGTPGSTSTNSPFVDLGTSSNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQ
EASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSNT
QSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 2076 bp        

>NTDB_id=373722 FFH90_RS03045 WP_064615925.1 663552..665627(+) (pilQ) [Pseudomonas sp. ATCC 43928]
ATGAACAGGATTTTCTCAACCTTCGGTATTTCGCTATGGATAGCGTTTCTGTCGCCGTTGGTACAGGCGGCCAACCTCAA
AGCGCTGGATGTCGCCGCGCTGCCTGGTGACCGTGTCGAGCTGAAGTTGTCGTTCGACGGGCCGCCACCGCAGCCTCATG
GCTATACGACTGAGCAGCCTGCGCGGATTGCGCTGGATTTGCCGGGAGTTACCAGTCAGCTGGCGAACAAGAGTCGTGAC
CTGGGCGGCGGCAATGCCCGCAGCGCCACGGTGGTGGAAGCCAAGGACCGCACGCGGCTGATCATCGGTCTGACTCAACT
GACTCCGTATAGCGCACGTGTCGAAGGCAACAACCTGTTCGTGGTCGTCGGGCAGGCGGCCGGTAACAAGGCGTCAAAAC
CGATTGCCAGCGCACCACGCGCGGCCACGGCGGTACCCGCACCCGCCAGAGCCAGTGCTCCCGTGGGCAAGGCTATTCGT
GGCGTGGATTTCCAGCGTGGCACCCAGGGTGAAGGCAACGTGGTTATCGATCTGTCGGATCCATCCATTGCCCCGGACAT
TCAGGAACGCGATGGCAAGATCATCGTCGGCTTCACCAAAACTCAATTGCCTGAGCGGTTGCGCGTACGCCTCGACGTCA
AGGATTTCGCTACCCCGGTGCAGTTCGTCAACGCCAGCGCCACGGGCGACCGGGCCATCATCAGCATCGAGCCCAGCGGC
GCCTTCGATTATTCGACCTACCAGACCGATAACAAACTGACCATCAGCATCCGGCCGATGACTGTCGATGACTTGCAAAA
ACGCAACGCCGAACGTTTTGCCTACAGCGGCGAAAAGCTCTCGTTGAATTTCCAGGACATCGATGTGCGCTCGGTGCTGC
AATTGATCGCCGATTTCACCAACCTCAATCTGGTGGCCAGTGACACGGTGCAGGGCGGCATCACGTTGCGCCTGCAGAAT
GTGCCGTGGGATCAGGCGCTGGACCTGGTGTTGAAAACCAAAGGCCTGGATAAGCGCAAGATTGGCAATGTGTTGCTGGT
GGCGCCGGCCGATGAAATCGCCGCCCGGGAGCGTCAGGAGCTGGAGTCGCAGAAACAAATCGCCGAACTGGCGCCTCTGC
GTCGCGAGCTGTTGCAGGTCAACTACGCCAAGGCTGCCGATATCGCCAAGCTGTTCCAGTCGGTGACCAGCGCCGAGGCG
AAAGTCGACGAGCGCGGTTCAATCACTGTCGATGAGCGGACCAACAACATCATTGCCTACCAGACCCAGGACCGCCTCGA
CGAGCTGCGTCGCATCGTTGCACAGCTGGATATTCCAGTGCGTCAGGTGATGATCGAGGCGCGCATCGTTGAAGCCAACG
TCGATTACGACAAAAGCCTCGGCGTGCGCTGGGGCGGTTCGGTGCAGAAGGGTAATTGGAACGGCTCCGGGGTCAACGGC
TCTTCGACCACCGTCGGCACGCCGGGCAGCACCAGCACCAACTCGCCGTTCGTCGACCTTGGGACTTCCTCCAACACCTC
GGGGATCGGCATCGCCTTCATTACCGACAATGTGCTGCTGGACCTTGAGCTGACGGCCATGGAAAAAACCGGCAACGGGG
AAATCGTCTCGCAGCCCAAGGTGGTCACGTCAGACAAGGAAACCGCGAAAATCCTCAAGGGCACCGAAATCCCCTATCAG
GAAGCCAGCTCCAGCGGTGCCACTTCGGTGTCGTTCAAGGAGGCCTCGCTGTCCCTGGAAGTGACGCCGCAGATCACCCC
GGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGACTACCTGAACAAAGTGCAGGATGTACCGCCGA
TCAAGAAAAACGAGGTCAACGCCAAGGTATTGGTCAACGACGGCGAGACCATCGTGATTGGTGGGGTTTTCTCAAATACT
CAGAGCAAGGTTGTAGATAAGGTGCCATTTCTCGGTGATGTACCGTATCTTGGCCGCCTTTTCCGGCGTGACGTGGTTTC
GGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAATCAGGCGATTGCTGTGAGTCATTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(303-349)

Bacterial type II and III secretion system protein (Secretin)

(526-682)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

AMIN domain (AMIN)

(37-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2W7LEI8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.829

100

0.753
  comQ Acinetobacter baylyi ADP1

41.541

100

0.437
  pilQ Acinetobacter baumannii strain A118

39.309

100

0.428
  pilQ Acinetobacter baumannii D1279779

39.309

100

0.428
  pilQ Legionella pneumophila strain Lp02

37.666

97.974

0.369
  pilQ Legionella pneumophila strain ERS1305867

37.666

97.974

0.369

Multiple sequence alignment