Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G5571_RS15320 Genome accession   NZ_CP049314
Coordinates   3202334..3203824 (-) Length   496 a.a.
NCBI ID   WP_001166321.1    Uniprot ID   V5VGM1
Organism   Acinetobacter baumannii strain Ex003     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3197334..3208824
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G5571_RS15290 (G5571_03052) gigA 3198328..3199296 (+) 969 WP_000273589.1 RsbU family protein phosphatase GigA -
  G5571_RS15295 (G5571_03053) gigB 3199399..3199920 (+) 522 WP_000103065.1 anti-anti-sigma factor GigB -
  G5571_RS15300 (G5571_03054) - 3199947..3200771 (+) 825 WP_000177091.1 carbon-nitrogen hydrolase family protein -
  G5571_RS15305 (G5571_03055) - 3200786..3201163 (+) 378 WP_000266123.1 DUF4951 domain-containing protein -
  G5571_RS15310 (G5571_03056) - 3201323..3201760 (+) 438 WP_000371731.1 MarR family winged helix-turn-helix transcriptional regulator -
  G5571_RS15315 (G5571_03057) - 3201848..3202279 (+) 432 WP_000051122.1 organic hydroperoxide resistance protein -
  G5571_RS15320 (G5571_03058) pilB 3202334..3203824 (-) 1491 WP_001166321.1 type II secretion system ATPase GspE Machinery gene
  G5571_RS15325 (G5571_03059) - 3203921..3204742 (+) 822 WP_000462729.1 hypothetical protein -
  G5571_RS15330 (G5571_03060) - 3204752..3205669 (+) 918 WP_000785635.1 mechanosensitive ion channel family protein -
  G5571_RS15335 (G5571_03061) - 3205659..3208466 (-) 2808 WP_000276867.1 DUF2339 domain-containing protein -

Sequence

Protein


Download         Length: 496 a.a.        Molecular weight: 55329.22 Da        Isoelectric Point: 6.2037

>NTDB_id=373476 G5571_RS15320 WP_001166321.1 3202334..3203824(-) (pilB) [Acinetobacter baumannii strain Ex003]
MQILKQIQVPYSFAKRHGVLFRYDGDQVFIVRRQNTEKIALQEARRILGKPAHYQLCTEQEFNSLLSTSYAGDTGESQQV
AAGLEDHPDLLSLADQVPETEDLMDQEDDAPIVRLINALLSEAIRVGASDIHIEAFEKKLSVRLRVDGQLREIVQPRREL
APLLVSRIKVMAKLDIAEKRVPQDGRISLRLAGREVDVRVSTLPSSHGERVVMRLLDKQAGRLNMTHLGLMANDYERLTQ
LVHRPHGIILVTGPTGSGKTTTLYAALSDLNDNTRNILTAEDPIEYQLEGIGQTQVNTKVDMTFARALKAMLRQDPDVVM
VGEIRDLETAEIAVQASLTGHLVLSTLHTNTAIGAVTRLKDMGIEPFLLSSSLIGVVAQRLVRTLCPHCMTWREADTFEK
QVFQHISHEPSLKLPEAQGCDQCSHLGFNGRTAIYEIVPIDEPMRRLIHGNAAEFELENHARQYSGSIRDDGLRKVLSGK
TTLEEVLRVTNEASEA

Nucleotide


Download         Length: 1491 bp        

>NTDB_id=373476 G5571_RS15320 WP_001166321.1 3202334..3203824(-) (pilB) [Acinetobacter baumannii strain Ex003]
ATGCAAATATTGAAACAAATACAAGTTCCATATAGTTTTGCCAAGCGTCATGGTGTTTTATTTCGTTATGACGGTGATCA
AGTTTTTATTGTTAGACGTCAAAATACAGAAAAAATTGCATTGCAGGAAGCAAGACGAATTTTGGGGAAACCTGCACACT
ATCAATTATGCACTGAACAAGAATTCAATAGCCTATTAAGTACCAGTTATGCAGGTGATACAGGTGAATCTCAACAAGTC
GCTGCGGGCTTAGAAGACCATCCGGATTTATTAAGCCTTGCAGATCAGGTGCCTGAAACAGAAGACTTAATGGATCAAGA
GGATGACGCGCCTATTGTTCGTCTAATCAATGCTCTTCTCTCTGAGGCAATTCGTGTTGGAGCTTCAGATATCCATATTG
AAGCCTTTGAGAAAAAGCTATCAGTTCGTTTACGGGTAGATGGTCAATTACGTGAAATTGTGCAGCCACGCCGTGAACTG
GCCCCGTTACTTGTTTCGCGTATTAAAGTTATGGCGAAACTCGATATTGCCGAAAAGCGTGTACCGCAAGATGGGCGTAT
TTCTTTACGTCTTGCTGGTCGTGAAGTCGATGTCCGTGTTTCAACTTTGCCATCGTCACATGGTGAAAGGGTGGTCATGC
GTTTACTCGATAAACAAGCGGGTCGCCTTAATATGACTCACCTAGGTTTAATGGCAAATGACTATGAACGTTTAACTCAG
CTAGTACATCGTCCGCACGGTATTATTTTGGTCACAGGGCCTACTGGTTCGGGGAAAACGACGACTTTATATGCTGCCTT
ATCTGATCTAAATGACAATACCCGTAACATCTTAACTGCCGAAGATCCAATTGAGTATCAGCTTGAAGGTATTGGCCAAA
CTCAAGTGAATACCAAAGTGGATATGACATTTGCTCGTGCTTTAAAGGCAATGTTACGTCAAGATCCAGATGTGGTCATG
GTGGGGGAGATTCGTGATCTGGAAACTGCTGAAATTGCGGTTCAAGCATCTTTAACAGGTCATTTGGTTTTATCGACGCT
ACATACCAATACTGCTATTGGTGCAGTGACTCGTCTTAAAGATATGGGGATTGAACCCTTCTTATTATCAAGTTCTTTAA
TTGGGGTGGTTGCGCAGCGTTTGGTTCGTACTTTATGTCCACATTGTATGACATGGCGTGAAGCCGATACTTTTGAGAAG
CAAGTTTTTCAGCATATTTCCCATGAACCAAGTTTAAAGTTACCAGAGGCTCAAGGGTGTGATCAGTGTTCTCATTTAGG
GTTTAATGGCCGTACAGCGATTTATGAAATTGTACCTATTGATGAACCTATGCGCCGTCTCATTCATGGTAATGCAGCTG
AATTTGAATTAGAAAATCATGCTCGTCAATATTCTGGTTCTATTCGCGATGATGGTTTACGTAAGGTGCTCTCAGGTAAA
ACTACACTTGAAGAAGTGCTTCGTGTAACCAATGAAGCAAGTGAAGCATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(118-384)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB V5VGM1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

41.598

98.387

0.409
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.057

99.194

0.407
  pilF Thermus thermophilus HB27

39.351

99.395

0.391
  pilB Vibrio campbellii strain DS40M4

48.371

80.444

0.389
  pilB/pilB1 Synechocystis sp. PCC 6803

40.041

97.177

0.389
  pilB Vibrio parahaemolyticus RIMD 2210633

48

80.645

0.387
  pilB Vibrio cholerae strain A1552

48

80.645

0.387
  pilF Neisseria gonorrhoeae MS11

48.477

79.435

0.385
  pilB Acinetobacter baumannii D1279779

47.146

81.25

0.383
  pilB Acinetobacter baylyi ADP1

46.787

78.427

0.367