Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FOG18_RS08205 Genome accession   NZ_CP041668
Coordinates   1818087..1819808 (-) Length   573 a.a.
NCBI ID   WP_143867707.1    Uniprot ID   -
Organism   Legionella israelensis strain L18-01051     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1813087..1824808
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOG18_RS08180 (FOG18_08180) - 1813582..1814397 (-) 816 WP_143867700.1 pyruvate, water dikinase regulatory protein -
  FOG18_RS08185 (FOG18_08185) - 1814763..1815170 (+) 408 WP_143867702.1 hypothetical protein -
  FOG18_RS08190 (FOG18_08190) - 1815182..1815772 (+) 591 WP_058502285.1 hypothetical protein -
  FOG18_RS08195 (FOG18_08195) - 1815997..1816857 (-) 861 WP_143867704.1 A24 family peptidase -
  FOG18_RS08200 (FOG18_08200) pilC 1816861..1818081 (-) 1221 WP_143867706.1 type II secretion system F family protein Machinery gene
  FOG18_RS08205 (FOG18_08205) pilB 1818087..1819808 (-) 1722 WP_143867707.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FOG18_RS08210 (FOG18_08210) - 1819977..1820756 (+) 780 WP_185967261.1 methyltransferase domain-containing protein -
  FOG18_RS08215 (FOG18_08215) - 1820882..1821208 (+) 327 WP_058502289.1 DUF1820 family protein -
  FOG18_RS08220 (FOG18_08220) - 1821331..1821906 (+) 576 WP_058502290.1 hypoxanthine-guanine phosphoribosyltransferase -
  FOG18_RS08225 (FOG18_08225) - 1821928..1823064 (-) 1137 WP_143867711.1 HlyD family type I secretion periplasmic adaptor subunit -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 63202.60 Da        Isoelectric Point: 5.9194

>NTDB_id=372938 FOG18_RS08205 WP_143867707.1 1818087..1819808(-) (pilB) [Legionella israelensis strain L18-01051]
MATLAEEQRLQGIGQLLVLEQLLEKDKTLKYQQLASADNVTLLQYLVNNSILSAVNIASAIARNFGVPLLDLDCIEFDSI
PSTLVSEKLIRRHHMIPLFHRGNHLYLATDDPSQQASLKEIQFHTGLHAHPVVVETDKLAQLIEHLLREKESQGLHDYVD
ESVDLDGLEISSEEEEHDIESAASASEDAPVVKFVNKILLDAIKNSVSDIHFEPYEQTYRIRYRQDGVLTEVASPPVSLS
GRITARIKVMSNLDISERRVPQDGRFKMKLSKSRAIDFRVSTCPTVGGEKVVMRILDPGSIKLGIEALGFSDIQKTHFLK
SIERPQGMILVTGPTGSGKTVTLYTAMNILNTKERNISTAEDPVEIKVPGINQVNINPKAGLTFSGALRSFLRQDPDIIM
VGEIRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLTRLVNMGVPSFNIASSVSLIIAQRLARRLCEQCKTKRDDITEKG
MIELGFSEEEAKMPIFKAVGCNQCNGGYRGRVGLFEVMPMSKAIGRLIMSGGNSLDILKQGQDEGMLTIFQSGLKKILEG
VTTIEEVNRVTVD

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=372938 FOG18_RS08205 WP_143867707.1 1818087..1819808(-) (pilB) [Legionella israelensis strain L18-01051]
ATGGCAACACTCGCAGAAGAACAACGATTGCAAGGCATAGGCCAATTATTGGTTCTTGAGCAACTCCTGGAAAAAGACAA
GACTCTGAAATATCAGCAATTAGCCAGTGCCGATAATGTAACTTTACTCCAATATCTGGTCAATAACAGTATACTCAGTG
CCGTTAATATTGCTTCCGCGATTGCCAGAAATTTTGGTGTACCTTTGTTGGATTTGGACTGTATCGAGTTTGATTCTATA
CCCTCCACACTCGTCAGTGAAAAACTTATCCGGCGCCATCATATGATTCCACTTTTTCACCGCGGTAATCATTTATACCT
TGCAACAGATGATCCCAGTCAACAGGCATCTCTGAAAGAAATACAGTTTCACACCGGTCTGCATGCTCATCCTGTTGTCG
TTGAAACAGACAAGTTGGCACAACTTATTGAGCATTTATTGCGGGAAAAAGAAAGCCAGGGTCTGCATGATTATGTCGAT
GAGTCCGTTGACCTCGATGGCCTTGAAATCAGTTCGGAAGAAGAAGAGCATGATATTGAATCAGCTGCCTCTGCTTCAGA
AGATGCCCCTGTAGTCAAATTTGTAAATAAAATTCTGCTTGATGCCATTAAAAATTCAGTATCGGATATTCATTTTGAAC
CTTATGAACAAACATACAGAATCCGATATCGCCAAGATGGTGTTCTAACTGAAGTCGCATCACCTCCTGTCAGTCTTTCC
GGACGTATCACTGCAAGAATCAAGGTCATGTCAAATCTGGATATTTCTGAAAGACGTGTGCCTCAGGATGGACGCTTTAA
AATGAAATTATCAAAATCGAGAGCTATAGATTTCAGGGTAAGTACCTGCCCCACCGTGGGAGGCGAAAAGGTGGTTATGC
GTATCCTTGATCCTGGAAGTATAAAATTAGGAATCGAAGCTTTGGGATTTAGCGATATTCAAAAAACTCATTTTTTAAAA
TCCATCGAACGCCCGCAAGGTATGATATTGGTAACAGGGCCTACAGGAAGCGGTAAAACCGTAACTTTATATACAGCCAT
GAATATTCTCAATACCAAAGAGAGGAACATCTCTACCGCTGAAGATCCCGTTGAAATTAAAGTCCCCGGTATCAATCAAG
TCAATATTAACCCTAAAGCGGGACTCACTTTTTCTGGTGCACTTCGTTCATTTTTACGGCAAGATCCTGACATCATCATG
GTTGGAGAAATTAGGGATCTTGAAACAGCGGAAATTGCAGTAAAAGCAGCTCAAACAGGCCATCTTGTTTTATCCACTCT
TCACACAAACAGTGCCGCAGAAACATTGACTCGCCTGGTTAACATGGGTGTGCCTTCTTTCAATATTGCAAGCTCGGTAA
GCTTAATCATCGCTCAGCGCCTGGCAAGACGCCTCTGTGAACAATGTAAAACAAAAAGAGACGATATCACTGAGAAAGGA
ATGATTGAATTAGGATTTAGCGAAGAAGAAGCTAAAATGCCCATATTCAAAGCTGTGGGTTGTAATCAATGCAATGGTGG
CTATCGGGGCAGAGTAGGATTATTTGAAGTCATGCCAATGTCAAAGGCAATAGGAAGACTCATTATGTCAGGAGGGAATT
CCTTGGATATACTCAAACAAGGTCAGGATGAGGGCATGTTAACTATTTTTCAATCCGGATTGAAAAAAATATTGGAAGGG
GTAACGACGATTGAGGAGGTCAACCGGGTAACCGTTGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-152)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

75.436

100

0.756
  pilB Acinetobacter baylyi ADP1

53.68

97.208

0.522
  pilB Acinetobacter baumannii D1279779

55.556

92.67

0.515
  pilF Neisseria gonorrhoeae MS11

47.627

99.302

0.473
  pilB Vibrio cholerae strain A1552

48.519

94.241

0.457
  pilB Vibrio parahaemolyticus RIMD 2210633

47.08

95.637

0.45
  pilB Vibrio campbellii strain DS40M4

47.681

94.066

0.449
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.927

96.161

0.384
  pilF Thermus thermophilus HB27

38.692

93.368

0.361

Multiple sequence alignment