Detailed information NTG0372411

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: FK728_RS16595
• Genome accession: NZ_CP041587
• Coordinates: 3498318..3499376 (-)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain J9
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3493318..3504376

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FK728_RS16570 (FK728_03285)	aroK	3493593..3494162 (-)	570	WP_000643712.1	shikimate kinase AroK	-
FK728_RS16575 (FK728_03286)	pilQ	3494174..3496339 (-)	2166	WP_001017033.1	type IV pilus secretin PilQ family protein	Machinery gene
FK728_RS16580 (FK728_03287)	pilP	3496402..3496929 (-)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
FK728_RS16585 (FK728_03288)	pilO	3496940..3497680 (-)	741	WP_000076100.1	type 4a pilus biogenesis protein PilO	Machinery gene
FK728_RS16590 (FK728_03289)	pilN	3497677..3498318 (-)	642	WP_000201224.1	PilN domain-containing protein	Machinery gene
FK728_RS16595 (FK728_03290)	pilM	3498318..3499376 (-)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
FK728_RS16600 (FK728_03291)	ponA	3499538..3502093 (+)	2556	WP_000736674.1	penicillin-binding protein PBP1a	-
FK728_RS16605 (FK728_03292)	-	3502111..3502935 (-)	825	WP_001981571.1	putative RNA methyltransferase	-
FK728_RS16610 (FK728_03293)	-	3502938..3503270 (-)	333	WP_000169597.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=372411 FK728_RS16595 WP_002014066.1 3498318..3499376(-) (pilM) [Acinetobacter baumannii strain J9]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=372411 FK728_RS16595 WP_002014066.1 3498318..3499376(-) (pilM) [Acinetobacter baumannii strain J9]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCCGATCGTCTTG
CGAATCCAAATCGTGTAAATGTGCTGTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGCCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTTTTCGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTACGGTTTGTCTTTAGAAGAA
GCAAGCCGAGCGAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTCGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCTAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423